FastQCFastQC Report
Fri 10 Feb 2017
SRR2031453_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031453_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683951
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC33150.48468384431048417No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA21400.31288791156091594No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC20420.29855939972307954No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT15640.2286713521875105No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT13810.20191504946991817No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT13520.19767497964035435No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT12450.18203058406230857No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA12370.1808609096265668No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG12080.176620839797003No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA11290.1650703047440533No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA10650.15571290925811937No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT10440.1526425138642973No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG8740.12778693210478528No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC8640.1263248390601081No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG8540.12486274601543094No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT7860.11492051331162612No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC7820.11433567609375525No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC7440.10877972252398198No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG7390.1080486760016434No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA7380.10790246669717567No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT7120.10410102478101502No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA7040.10293135034527326No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC6890.1007382107782575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11900.039.8941231
GGTATCA8950.036.0696371
GTACATG25600.032.638381
ACATGGG25150.030.7706953
ATCAACG16000.028.7832243
TCAACGC16000.028.7832244
AACGCAG16150.028.495026
CAACGCA16200.028.4278775
CATGGGA16550.026.9660644
TCCGTCA458.957883E-426.38410824-25
CGCAGAG17900.025.7091968
AGGTCGT1501.0702515E-625.3027956
ACGCAGA18600.024.7416467
CATATAG2501.4551915E-1124.688293
TATCAAC18850.024.4295942
TAATACT13750.024.1703554
ATGGGAT10250.024.0861385
CTTAATA14200.023.7370032
GGTCGTT1401.794787E-523.721377
GATTGGC3650.023.3964219
Produced by FastQC (version 0.11.5)