##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031453_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683951
Sequences flagged as poor quality	0
Sequence length	101
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95265742721335	34.0	33.0	34.0	31.0	34.0
2	33.02479417385164	34.0	33.0	34.0	31.0	34.0
3	33.188272259270036	34.0	34.0	34.0	31.0	34.0
4	36.14981482591589	37.0	37.0	37.0	35.0	37.0
5	36.15523188064642	37.0	37.0	37.0	35.0	37.0
6	36.222583196749476	37.0	37.0	37.0	35.0	37.0
7	36.24751188316122	37.0	37.0	37.0	35.0	37.0
8	36.32166631820116	37.0	37.0	37.0	36.0	37.0
9	38.27759006127632	39.0	39.0	39.0	38.0	39.0
10-11	38.34522502343005	39.0	39.0	39.0	37.5	39.0
12-13	38.40715782270952	39.0	39.0	39.0	38.0	39.0
14-15	40.0684654310031	41.0	41.0	41.0	39.0	41.0
16-17	40.082925531214954	41.0	41.0	41.0	39.0	41.0
18-19	40.11470193040145	41.0	41.0	41.0	39.0	41.0
20-21	40.06222083160928	41.0	41.0	41.0	39.0	41.0
22-23	40.010177629683994	41.0	40.0	41.0	39.0	41.0
24-25	39.91499025514986	41.0	40.0	41.0	39.0	41.0
26-27	39.84879764778471	41.0	40.0	41.0	38.0	41.0
28-29	39.8034230522362	41.0	40.0	41.0	38.0	41.0
30-31	39.67256206950498	41.0	40.0	41.0	38.0	41.0
32-33	39.54588779020719	41.0	40.0	41.0	38.0	41.0
34-35	39.51870455632056	41.0	40.0	41.0	38.0	41.0
36-37	39.441321819837974	41.0	40.0	41.0	38.0	41.0
38-39	39.28592764686359	41.0	40.0	41.0	37.0	41.0
40-41	39.21668218922116	41.0	40.0	41.0	37.0	41.0
42-43	39.39338125099605	41.0	40.0	41.0	38.0	41.0
44-45	39.40048921633275	41.0	40.0	41.0	38.0	41.0
46-47	39.299587982180014	41.0	40.0	41.0	37.0	41.0
48-49	39.194061416680434	41.0	40.0	41.0	37.0	41.0
50-51	38.27145365676781	40.0	38.5	40.5	35.5	40.5
52-53	38.367260958752894	40.0	39.0	40.5	35.5	41.0
54-55	38.858298328389026	41.0	39.5	41.0	36.0	41.0
56-57	38.939656495860085	41.0	40.0	41.0	36.0	41.0
58-59	39.03953572697459	41.0	40.0	41.0	36.0	41.0
60-61	38.92658538404067	41.0	39.5	41.0	35.5	41.0
62-63	38.76598396668767	41.0	39.0	41.0	35.0	41.0
64-65	38.598382778883284	41.0	39.0	41.0	35.0	41.0
66-67	38.431408097948534	41.0	38.5	41.0	35.0	41.0
68-69	38.202638785527036	40.0	37.5	41.0	35.0	41.0
70-71	37.89760012047647	39.5	37.0	41.0	35.0	41.0
72-73	37.57833309696163	39.0	36.5	41.0	35.0	41.0
74-75	37.19661130695035	39.0	36.0	41.0	34.5	41.0
76-77	36.81077518711136	38.0	35.5	40.0	34.0	41.0
78-79	36.34685964345399	37.0	35.0	39.0	34.0	41.0
80-81	35.839851831490854	37.0	35.0	39.0	34.0	40.0
82-83	35.42048041453262	36.0	35.0	38.0	34.0	39.0
84-85	34.98376053255277	36.0	35.0	37.0	33.0	39.0
86-87	34.645723889576885	35.5	35.0	37.0	33.0	38.0
88-89	34.37314003488554	35.0	35.0	36.5	33.0	37.0
90-91	34.09323328717993	35.0	35.0	36.0	33.0	37.0
92-93	33.95602828272786	35.0	35.0	36.0	33.0	36.5
94-95	33.805984639250475	35.0	35.0	36.0	32.0	36.0
96-97	33.6763130692111	35.0	35.0	35.0	32.0	36.0
98-99	33.57028281265763	35.0	35.0	35.0	32.0	36.0
100-101	33.2401151544482	35.0	34.5	35.0	31.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.08601415138598156
1101	2	0.07820251784140453
1101	3	-0.07329443085633613
1101	4	-0.06118817172024649
1101	5	-0.0706289165129732
1101	6	0.07422370679803691
1101	7	-0.059020820215963
1101	8	-0.03562483772034852
1101	9	-0.021654428735047304
1101	10-11	-0.05968703286512067
1101	12-13	-0.21055808185138858
1101	14-15	-0.21234587517550807
1101	16-17	-0.18426378712428715
1101	18-19	-0.10826095703551886
1101	20-21	-0.20263425831189608
1101	22-23	-0.21779540328737568
1101	24-25	-0.2022106800836525
1101	26-27	-0.1848140178187947
1101	28-29	-0.13694672332352553
1101	30-31	-0.15139173170856424
1101	32-33	-0.09924194932110453
1101	34-35	-0.13256177327767915
1101	36-37	-0.1252213166604932
1101	38-39	-0.19651658293251018
1101	40-41	-0.06622443198024541
1101	42-43	-0.06618969333565872
1101	44-45	-0.11662168757420233
1101	46-47	-0.038320722345090985
1101	48-49	0.0484258844677683
1101	50-51	0.09644809896055762
1101	52-53	-0.10952352838583579
1101	54-55	-0.04551243008157968
1101	56-57	-0.11618550201630029
1101	58-59	-0.16563201936982352
1101	60-61	-0.23126510139247358
1101	62-63	-0.23124178365858228
1101	64-65	-0.35678810087065216
1101	66-67	-0.29659619107114565
1101	68-69	-0.2982277658704291
1101	70-71	-0.4336320814669534
1101	72-73	-0.40770941895660684
1101	74-75	-0.2904300389227501
1101	76-77	-0.4507336212838453
1101	78-79	-0.31812580393596335
1101	80-81	-0.4516420780035659
1101	82-83	-0.3968329872532337
1101	84-85	-0.30725919403111845
1101	86-87	-0.23188464680286813
1101	88-89	-0.18877825729462216
1101	90-91	-0.2058087162612381
1101	92-93	-0.23182345722896258
1101	94-95	-0.3174965141007746
1101	96-97	-0.3369820758541948
1101	98-99	-0.3384314561329731
1101	100-101	-0.4782556549586445
1102	1	-0.05917559242221415
1102	2	0.007211231583781341
1102	3	0.02565405084887118
1102	4	-0.06653438244811127
1102	5	-0.04467364453902434
1102	6	-0.009900662334572985
1102	7	-0.047674650165788535
1102	8	-0.018072414346946175
1102	9	-0.10698169528142643
1102	10-11	0.08694732409323791
1102	12-13	0.10416084983624074
1102	14-15	0.17776479719766058
1102	16-17	0.14750926623032257
1102	18-19	0.1592849288519389
1102	20-21	0.1734048387596019
1102	22-23	0.10782950773016609
1102	24-25	0.027442484495381336
1102	26-27	0.07045011114110622
1102	28-29	-0.08713834430043477
1102	30-31	0.017734482447856692
1102	32-33	0.02234802537156355
1102	34-35	0.1357845914443132
1102	36-37	0.07957923190721061
1102	38-39	0.056564025721961286
1102	40-41	-0.053978994419054516
1102	42-43	-0.04926632138757725
1102	44-45	-0.14773721512026583
1102	46-47	-0.028977928543206133
1102	48-49	0.06780735113095204
1102	50-51	0.06957031508112976
1102	52-53	0.06061962802860421
1102	54-55	0.1550431109462309
1102	56-57	0.1171548046353692
1102	58-59	0.12551564355159428
1102	60-61	0.12498865766195166
1102	62-63	0.19065123982697685
1102	64-65	0.3168722229818641
1102	66-67	0.2513017892815341
1102	68-69	0.26288901106183005
1102	70-71	0.17337348847107847
1102	72-73	0.16421309585442856
1102	74-75	0.19625092304651304
1102	76-77	0.0717437081676735
1102	78-79	0.17213624755618184
1102	80-81	0.15199741306041403
1102	82-83	0.07012220045977813
1102	84-85	0.16524559286136764
1102	86-87	0.172560553084395
1102	88-89	0.2590831131367608
1102	90-91	0.20585532088330893
1102	92-93	0.2219027644818823
1102	94-95	0.0669599593178063
1102	96-97	0.04169235444021524
1102	98-99	0.0401514493582269
1102	100-101	0.30340103114615147
1103	1	0.26005408433742616
1103	2	0.23625457646817694
1103	3	0.17221429626158624
1103	4	0.13699342531192826
1103	5	0.18100095638126845
1103	6	0.18642950865901042
1103	7	0.143329724088602
1103	8	0.16607801455722182
1103	9	0.20160774969954076
1103	10-11	0.1344548512192958
1103	12-13	0.20452584443943778
1103	14-15	0.25758357808727794
1103	16-17	0.16590953891111582
1103	18-19	0.0810582061342231
1103	20-21	0.24600041905841152
1103	22-23	0.24597641450742458
1103	24-25	0.1763105751617431
1103	26-27	0.1414053460442446
1103	28-29	0.20553335938644324
1103	30-31	0.07889167156334764
1103	32-33	0.20924798560561442
1103	34-35	0.2477839665508199
1103	36-37	0.0817649388889734
1103	38-39	0.23061958443354058
1103	40-41	0.2114104278385156
1103	42-43	0.26691557942478283
1103	44-45	0.22499328536260776
1103	46-47	0.2400941046329521
1103	48-49	0.27314308935739007
1103	50-51	0.16659496996950196
1103	52-53	0.16636438744460236
1103	54-55	0.015159284325761746
1103	56-57	0.08466744452629626
1103	58-59	0.1102923861522882
1103	60-61	0.20893741639625318
1103	62-63	0.2283124339416105
1103	64-65	0.34876593428107583
1103	66-67	0.10784186767725856
1103	68-69	0.22620208269734832
1103	70-71	0.2246602009632639
1103	72-73	0.1919043984733051
1103	74-75	0.15141907620222383
1103	76-77	0.1369115772808982
1103	78-79	0.19315255159526146
1103	80-81	0.25858719889237847
1103	82-83	0.3560223311193269
1103	84-85	0.44963887809684167
1103	86-87	0.2982436752794797
1103	88-89	0.32044907348442564
1103	90-91	0.3075723577520151
1103	92-93	0.13838575033656753
1103	94-95	0.1890442631296665
1103	96-97	0.1835403348980975
1103	98-99	0.12596068642009328
1103	100-101	0.1963155911643355
1104	1	0.2674148270429981
1104	2	0.3477046206768364
1104	3	0.2760051892766171
1104	4	0.3064010292022914
1104	5	0.20721669466597348
1104	6	0.16686717444149934
1104	7	0.20290090003909
1104	8	0.22464344338126807
1104	9	0.27100430142368026
1104	10-11	0.27510471859330465
1104	12-13	0.2260388859955853
1104	14-15	0.23610921911291882
1104	16-17	0.20007178471129095
1104	18-19	0.15879914335791767
1104	20-21	0.23004109103012382
1104	22-23	0.2323767239680805
1104	24-25	0.2858209641979954
1104	26-27	0.3086909340194879
1104	28-29	0.2158505565570792
1104	30-31	0.23737420914071095
1104	32-33	0.266719249973427
1104	34-35	0.26779943958353414
1104	36-37	0.3034603411878223
1104	38-39	0.20087267904008144
1104	40-41	0.27809367275452246
1104	42-43	0.21690452725855636
1104	44-45	0.15430915627330677
1104	46-47	0.2149946351369394
1104	48-49	0.26898194877383474
1104	50-51	0.12431490807737333
1104	52-53	0.11518733174168716
1104	54-55	0.2086550845026025
1104	56-57	0.3697857027049025
1104	58-59	0.3964440218728953
1104	60-61	0.31238569225832435
1104	62-63	0.4594463861962552
1104	64-65	0.43427654436065666
1104	66-67	0.4462813018063443
1104	68-69	0.35742445228178354
1104	70-71	0.4635748782400029
1104	72-73	0.26977243560858
1104	74-75	0.3403061230634066
1104	76-77	0.1422829300660453
1104	78-79	0.29563928899579395
1104	80-81	0.28278591684109244
1104	82-83	0.14401967859943454
1104	84-85	0.31300094706235626
1104	86-87	0.34226997943508763
1104	88-89	0.31985778259140574
1104	90-91	0.2693981756123165
1104	92-93	0.30683734184850664
1104	94-95	0.2403484187441549
1104	96-97	0.18119727565849075
1104	98-99	0.11858336546517734
1104	100-101	0.1220063515533667
1105	1	-0.025604462535923744
1105	2	0.04471708833150956
1105	3	0.03719373314864782
1105	4	-0.09797441187039624
1105	5	-0.00955582944600053
1105	6	0.05092386698443363
1105	7	0.13723987910283597
1105	8	0.13585551113003191
1105	9	0.06324436258576327
1105	10-11	0.12902144406965732
1105	12-13	0.14431546797629125
1105	14-15	0.13006064230746262
1105	16-17	0.16491829141559577
1105	18-19	0.1879099408173346
1105	20-21	0.1825816697174858
1105	22-23	0.19833326360581083
1105	24-25	0.24993970096581108
1105	26-27	0.2710821360895892
1105	28-29	0.3228327372204589
1105	30-31	0.22870713080619964
1105	32-33	0.2453125221446797
1105	34-35	0.18680379150072923
1105	36-37	0.16030284883308354
1105	38-39	0.19424923749691914
1105	40-41	0.1758309698610816
1105	42-43	0.30419281236794404
1105	44-45	0.2803897843608141
1105	46-47	0.29646076075443517
1105	48-49	0.3807277144760235
1105	50-51	0.29335601605196615
1105	52-53	0.2133774634753962
1105	54-55	0.21466102428147593
1105	56-57	0.3919937125849273
1105	58-59	0.28968972876538146
1105	60-61	0.23899119684548253
1105	62-63	0.234249373728602
1105	64-65	0.25568621267399294
1105	66-67	0.2991394528296425
1105	68-69	0.24431871245585768
1105	70-71	0.24231811512544965
1105	72-73	0.2361994527810154
1105	74-75	0.31739239688133125
1105	76-77	0.1934179571184984
1105	78-79	0.2222889008518294
1105	80-81	0.3382508074550685
1105	82-83	0.3809933318574963
1105	84-85	0.24667831709293608
1105	86-87	0.2207471024649692
1105	88-89	0.4110348585735366
1105	90-91	0.3720798975600985
1105	92-93	0.18493220188143766
1105	94-95	0.05730325290083016
1105	96-97	0.19166540080551187
1105	98-99	0.18129655059809124
1105	100-101	0.21427140683447732
1106	1	0.17591820890703502
1106	2	0.21137919746617229
1106	3	0.2682264243257251
1106	4	0.18853255887480458
1106	5	0.24913652347105142
1106	6	0.15862746333294098
1106	7	0.1375611851092131
1106	8	0.21758117504850816
1106	9	0.2435266561181635
1106	10-11	0.3215623593132406
1106	12-13	0.14263032051734825
1106	14-15	0.15497553039725886
1106	16-17	0.24539974909662732
1106	18-19	0.2485809462544708
1106	20-21	0.311769471938824
1106	22-23	0.3804180610463064
1106	24-25	0.22079770194112314
1106	26-27	0.24239589026542774
1106	28-29	0.36619423610158464
1106	30-31	0.3896142164012062
1106	32-33	0.3603605813196822
1106	34-35	0.35745943805500957
1106	36-37	0.42010837894242314
1106	38-39	0.3093132480339378
1106	40-41	0.3618318266771041
1106	42-43	0.37286912277232886
1106	44-45	0.4394043547906321
1106	46-47	0.32433918403066286
1106	48-49	0.3996745144049285
1106	50-51	0.44738920400002513
1106	52-53	0.5590605691600103
1106	54-55	0.5692666382493954
1106	56-57	0.5409794338371583
1106	58-59	0.5546202036146468
1106	60-61	0.29189388897963653
1106	62-63	0.3281197210581155
1106	64-65	0.43422638959738435
1106	66-67	0.48074001249457154
1106	68-69	0.5406015893523701
1106	70-71	0.3748272187956232
1106	72-73	0.5061625823470877
1106	74-75	0.43189469159869986
1106	76-77	0.5063062018761997
1106	78-79	0.6376589496629919
1106	80-81	0.4872012078342536
1106	82-83	0.4641933573796706
1106	84-85	0.5444651795511746
1106	86-87	0.43547066536118706
1106	88-89	0.6030471001044972
1106	90-91	0.6065432709541412
1106	92-93	0.5152268007502556
1106	94-95	0.6420403060917863
1106	96-97	0.38689867808120226
1106	98-99	0.41533835228164406
1106	100-101	0.4353219635370067
1107	1	0.23260521986497196
1107	2	0.3471757517269154
1107	3	0.22287893549246718
1107	4	0.3128751146237434
1107	5	0.30770179515107543
1107	6	0.21360368828327836
1107	7	0.16916726762124767
1107	8	0.1464870987775555
1107	9	0.16654128577645366
1107	10-11	0.17107254877166156
1107	12-13	0.12047595970897618
1107	14-15	0.17287518548414482
1107	16-17	0.13723440279495946
1107	18-19	0.14915184423693262
1107	20-21	0.10158976521037033
1107	22-23	0.05819989025177108
1107	24-25	0.0873457936833475
1107	26-27	0.0627167209400099
1107	28-29	0.13303127472464382
1107	30-31	0.2183702608604463
1107	32-33	0.1538695581763747
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2316	60-61	-0.6471659646617525
2316	62-63	-0.6797705128890641
2316	64-65	-0.5471018482019474
2316	66-67	-0.5860698752816731
2316	68-69	-0.5284561145198339
2316	70-71	-1.3615769436223388
2316	72-73	-0.8716702469525259
2316	74-75	-0.6155483903568921
2316	76-77	-0.59277854841649
2316	78-79	-0.6828338703984116
2316	80-81	-0.6961886295676578
2316	82-83	-0.6830346024022163
2316	84-85	-0.5331247839317967
2316	86-87	-0.43616850459549994
2316	88-89	-0.5230862494973607
2316	90-91	-0.5391188434310195
2316	92-93	-0.548355528146999
2316	94-95	-0.5146560796813873
2316	96-97	-0.5989796624131856
2316	98-99	-0.5565693935691556
2316	100-101	-0.5614497513106329
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	254.0
3	90.0
4	81.0
5	110.0
6	112.0
7	424.0
8	1026.0
9	857.0
10	614.0
11	496.0
12	533.0
13	541.0
14	618.0
15	628.0
16	662.0
17	824.0
18	909.0
19	1181.0
20	1486.0
21	1897.0
22	2033.0
23	1941.0
24	1730.0
25	1621.0
26	1574.0
27	1681.0
28	1865.0
29	2105.0
30	2599.0
31	3206.0
32	4172.0
33	5699.0
34	8769.0
35	14889.0
36	28746.0
37	84181.0
38	287897.0
39	215294.0
40	606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.676985033711084	25.081955462454086	14.9490274325888	22.29203207124603
2	12.101193219644594	24.582373124391868	43.4080284441323	19.908405211831237
3	14.374070178569601	32.65232980153569	33.06236550960431	19.91123451029039
4	10.083716411752484	20.275992029237834	45.63612475822899	24.00416680078069
5	9.828042192524249	42.35088525559496	37.540340344442505	10.280732207438284
6	23.000946721665294	45.192570795612006	21.08570099105809	10.720781491664606
7	22.04395736279241	34.91049435567791	28.981504654937485	14.064043626592198
8	21.806319586149957	39.16629827298996	24.39815207876501	14.629230062095072
9	25.115899153464095	16.70982540837343	24.147133005531835	34.02714243263064
10-11	20.05644716454685	29.45296874337024	33.800556278174206	16.69002781390871
12-13	21.20746894947144	27.82709567753822	32.500418736884754	18.46501663610558
14-15	19.752441337642264	29.44453714111751	29.370184651772753	21.432836869467472
16-17	18.22431000330625	32.936680935229695	30.169086860730825	18.66992220073323
18-19	18.240146721472712	31.783363522485374	31.577292395039375	18.39919736100254
20-21	19.233480717448124	31.653749226635618	31.095829902252593	18.01694015366366
22-23	19.364973677760176	31.075461965369517	30.86949363985908	18.690070717011228
24-25	19.206584863690594	31.863975513307206	30.055305999430164	18.874133623572032
26-27	18.67491985225128	31.75879720880018	31.014487021448172	18.55179591750037
28-29	18.948523603384366	30.667085429894865	31.301306731618894	19.08308423510188
30-31	19.680237603056153	31.282425296875676	30.276156436657125	18.761180663411047
32-33	19.17409965256561	31.06475233393559	30.971336296270245	18.789811717228556
34-35	18.90745962573699	31.294979309334604	31.311092393891677	18.48646867103673
36-37	19.484522113620468	30.81793113045516	30.05299065797039	19.644556097953984
38-39	19.06765689867658	31.421279925673986	30.740080216999143	18.770982958650293
40-41	18.894447560620193	30.65169730689189	31.904668454358927	18.549186678128986
42-43	18.947677281650865	30.78111268917567	30.351303513035134	19.919906516138333
44-45	19.10692885895434	31.00588659632406	30.42286042050461	19.464324124216986
46-47	19.83150580890821	30.518682309280642	30.452025105839347	19.1977867759718
48-49	19.01790956059867	30.562204722103882	30.537682107861436	19.882203609436008
50-51	18.74880117605544	30.968898663320456	30.3665216608269	19.915778499797206
52-53	18.92800546007903	30.295602937428782	31.09469604876577	19.681695553726417
54-55	19.215127549468612	30.862522728921743	30.70282479913167	19.21952492247798
56-57	19.734976986848476	30.551780872895467	29.91255515208166	19.800686988174398
58-59	18.640236875284636	30.21067783877661	30.555242344678724	20.593842941260025
60-61	19.90121259740945	30.50440358150144	30.3155215986584	19.278862222430707
62-63	19.318920622588056	30.69189074290573	30.99226346015004	18.996925174356175
64-65	19.16981602378238	30.654125443995206	30.63132544252897	19.544733089693448
66-67	19.451159780417054	31.14524014871195	30.067386017035396	19.336214053835597
68-69	19.42229143284288	30.580380839005507	30.47751667607453	19.519811052077088
70-71	19.702081548678073	30.15456029755237	30.70617435807323	19.437183795696328
72-73	19.367441315499445	30.608942086242692	30.310530805930146	19.713085792327718
74-75	19.195550445614522	30.33049438679487	29.78418635987492	20.689768807715687
76-77	18.868677490122106	30.77457610736368	31.081017752380507	19.275728650133704
78-79	19.117169845182417	30.30556969721387	30.37479260596782	20.202467851635895
80-81	19.442071634975303	30.83571204936682	29.943519394998354	19.778696920659524
82-83	19.337139337139337	30.724057390724056	30.18161018161018	19.757193090526425
84-85	19.874054455728523	30.112314756866326	29.857653540560385	20.155977246844767
86-87	18.334356965806766	31.293910535010426	31.204641965608882	19.167090533573923
88-89	19.323221152472016	29.98146564328289	30.806827151183626	19.888486053061467
90-91	19.581822017262695	30.21880145555119	30.856117826986296	19.34325870019982
92-93	19.874328525447375	30.633227508577665	30.325859688823954	19.166584277151006
94-95	19.701632869152984	29.743862370051104	30.389789936242025	20.16471482455389
96-97	19.516132574842405	30.045904807924618	30.799490434668016	19.63847218256496
98-99	19.68001288735282	29.817233905453694	30.803409290609807	19.699343916583683
100-101	19.21746397622757	30.410414281623023	31.10338619432365	19.268735547825756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	126.5
2	108.5
3	104.5
4	99.5
5	97.0
6	93.5
7	115.5
8	148.5
9	161.0
10	153.0
11	142.5
12	139.0
13	155.0
14	159.5
15	155.0
16	191.0
17	228.5
18	237.5
19	250.5
20	290.5
21	339.0
22	394.0
23	449.5
24	537.5
25	734.5
26	1131.5
27	2066.5
28	3981.5
29	7628.5
30	12629.5
31	18039.5
32	25860.5
33	34350.5
34	39886.0
35	47600.5
36	61614.5
37	69886.5
38	62706.0
39	51648.5
40	41679.5
41	32030.5
42	25092.5
43	20095.5
44	16509.5
45	13303.0
46	10324.0
47	7905.0
48	6899.5
49	6308.0
50	5784.0
51	5519.0
52	5194.5
53	4835.0
54	4510.0
55	4106.5
56	3800.0
57	3535.5
58	3158.0
59	2702.5
60	2258.0
61	1929.0
62	1636.0
63	1448.5
64	1296.0
65	1085.5
66	958.0
67	849.5
68	762.0
69	656.0
70	560.0
71	481.0
72	388.5
73	338.0
74	284.5
75	230.0
76	175.5
77	139.5
78	118.0
79	89.5
80	67.0
81	42.5
82	34.5
83	27.0
84	17.0
85	14.0
86	11.5
87	7.0
88	6.0
89	10.0
90	9.0
91	3.5
92	2.5
93	2.0
94	1.5
95	1.5
96	2.0
97	1.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050880837954765765
2	0.07398190806066517
3	0.05146567517263663
4	0.06623281492387612
5	0.10263893173633784
6	0.07895302441256757
7	0.10205409451846696
8	0.11769849009651276
9	0.11901437383672223
10-11	0.0701804661445045
12-13	0.0508077333025319
14-15	0.10512448991228902
16-17	0.05804509387368394
18-19	0.03019222137258371
20-21	0.03655232611692943
22-23	0.08677522220159047
24-25	0.18926794463346058
26-27	0.1908762469826055
28-29	0.14438168816187125
30-31	0.1654358280052226
32-33	0.22223814279093093
34-35	0.1865630725008078
36-37	0.1876596422843157
38-39	0.22691684053389788
40-41	0.1658013512663919
42-43	0.09130771064008972
44-45	0.07807576858576126
46-47	0.0883104198985015
48-49	0.1327580484566877
50-51	0.14474721142304053
52-53	0.17208835135850375
54-55	0.2522841548590469
56-57	0.20564338673384497
58-59	0.15498186273578077
60-61	0.2601063526480698
62-63	0.2629574340851903
64-65	0.28269569018833224
66-67	0.1491334905570721
68-69	0.14789071146909646
70-71	0.15293493247323273
72-73	0.17084557227052816
74-75	0.1734042350987132
76-77	0.16273095587256983
78-79	0.2008184796864103
80-81	0.2976090392440394
82-83	0.3119375510818757
84-85	0.24446195707002405
86-87	0.25440418977382884
88-89	0.24921375946522487
90-91	0.19343490981079053
92-93	0.17815603749391404
94-95	0.16587445591862576
96-97	0.1485486533392012
98-99	0.16243853726363439
100-101	0.19175350280941178
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	683951.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.70290512348563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.38638350929709	22.037305065193593
2	13.944816347644974	9.120720119629299
3	6.116354666185217	6.000676990495313
4	3.2635722721969795	4.26913177525185
5	1.9619311386984046	3.2080423943833023
6	1.3111070097116444	2.5726204887202115
7	0.9270735082769768	2.1222597888575256
8	0.7044626551532808	1.8430380299613212
9	0.5187700116236228	1.5268757823933186
>10	3.33381490848055	20.80898036100165
>50	0.29031401750735525	6.609145950141422
>100	0.21920746403068855	13.641898133069327
>500	0.01675759539077578	3.466313536960626
>1k	0.005434895802413766	2.77299158394124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	3315	0.48468384431048417	No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA	2140	0.31288791156091594	No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC	2042	0.29855939972307954	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	1564	0.2286713521875105	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	1381	0.20191504946991817	No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT	1352	0.19767497964035435	No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT	1245	0.18203058406230857	No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA	1237	0.1808609096265668	No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG	1208	0.176620839797003	No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA	1129	0.1650703047440533	No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA	1065	0.15571290925811937	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	1044	0.1526425138642973	No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG	874	0.12778693210478528	No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC	864	0.1263248390601081	No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG	854	0.12486274601543094	No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT	786	0.11492051331162612	No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC	782	0.11433567609375525	No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC	744	0.10877972252398198	No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG	739	0.1080486760016434	No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA	738	0.10790246669717567	No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT	712	0.10410102478101502	No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA	704	0.10293135034527326	No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC	689	0.1007382107782575	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.4620930446771773E-4	0.0
8	0.0	0.0	0.0	1.4620930446771773E-4	0.0
9	0.0	0.0	0.0	1.4620930446771773E-4	0.0
10-11	0.0	0.0	0.0	1.4620930446771773E-4	0.0
12-13	0.0	0.0	0.0	1.4620930446771773E-4	0.0
14-15	0.0	0.0	0.0	2.1931395670157658E-4	0.0
16-17	0.0	0.0	0.0	3.655232611692943E-4	0.0
18-19	0.0	0.0	0.0	5.11732565637012E-4	0.0
20-21	0.0	0.0	0.0	5.848372178708709E-4	0.0
22-23	0.0	0.0	0.0	0.0012427790879756006	0.0
24-25	0.0	0.0	0.0	0.0027048721326527777	0.0
26-27	0.0	0.0	0.0	0.00445938378626539	0.0
28-29	0.0	0.0	0.0	0.009503604790401653	0.0
30-31	0.0	0.0	0.0	0.018934104928569444	0.0
32-33	0.0	0.0	0.0	0.03655232611692943	0.0
34-35	0.0	0.0	0.0	0.06513624514036824	0.0
36-37	0.0	0.0	0.0	0.10351618756314415	0.0
38-39	0.0	0.0	0.0	0.16390063030831156	0.0
40-41	0.0	0.0	0.0	0.23071828245005854	0.0
42-43	0.0	0.0	0.0	0.2923455042832016	0.0
44-45	0.0	0.0	0.0	0.3610638773830289	0.0
46-47	0.0	0.0	0.0	0.4446955995385634	0.0
48-49	0.0	0.0	0.0	0.5227713681243247	0.0
50-51	0.0	0.0	0.0	0.6134942415465435	0.0
52-53	1.4620930446771773E-4	0.0	0.0	0.6922279520024095	0.0
54-55	1.4620930446771773E-4	0.0	0.0	0.7699381973270014	0.0
56-57	1.4620930446771773E-4	0.0	0.0	0.858248617225503	0.0
58-59	1.4620930446771773E-4	0.0	0.0	0.9535039790862211	0.0
60-61	1.4620930446771773E-4	0.0	0.0	1.0559235968658574	0.0
62-63	1.4620930446771773E-4	0.0	0.0	1.153956935511462	0.0
64-65	2.9241860893543546E-4	0.0	0.0	1.2414632042353912	0.0
66-67	2.9241860893543546E-4	0.0	0.0	1.3356219963126015	0.0
68-69	2.9241860893543546E-4	0.0	0.0	1.4381878233967054	0.0
70-71	2.9241860893543546E-4	0.0	0.0	1.560053278670548	0.0
72-73	2.9241860893543546E-4	0.0	0.0	1.676655198983553	0.0
74-75	2.9241860893543546E-4	0.0	0.0	1.7915026076429452	0.0
76-77	2.9241860893543546E-4	0.0	0.0	1.906569330259039	0.0
78-79	2.9241860893543546E-4	0.0	0.0	2.0347948902772277	0.0
80-81	2.9241860893543546E-4	0.0	0.0	2.1787379505256954	0.0
82-83	2.9241860893543546E-4	0.0	0.0	2.3288218015618076	0.0
84-85	2.9241860893543546E-4	0.0	0.0	2.4707910361999614	0.0
86-87	2.9241860893543546E-4	0.0	0.0	2.603987712570053	0.0
88-89	2.9241860893543546E-4	0.0	0.0	2.7507818542556413	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1190	0.0	39.894123	1
GGTATCA	895	0.0	36.069637	1
GTACATG	2560	0.0	32.63838	1
ACATGGG	2515	0.0	30.770695	3
ATCAACG	1600	0.0	28.783224	3
TCAACGC	1600	0.0	28.783224	4
AACGCAG	1615	0.0	28.49502	6
CAACGCA	1620	0.0	28.427877	5
CATGGGA	1655	0.0	26.966064	4
TCCGTCA	45	8.957883E-4	26.384108	24-25
CGCAGAG	1790	0.0	25.709196	8
AGGTCGT	150	1.0702515E-6	25.302795	6
ACGCAGA	1860	0.0	24.741646	7
CATATAG	250	1.4551915E-11	24.68829	3
TATCAAC	1885	0.0	24.429594	2
TAATACT	1375	0.0	24.170355	4
ATGGGAT	1025	0.0	24.086138	5
CTTAATA	1420	0.0	23.737003	2
GGTCGTT	140	1.794787E-5	23.72137	7
GATTGGC	365	0.0	23.396421	9
>>END_MODULE