FastQCFastQC Report
Fri 10 Feb 2017
SRR2031453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683951
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC37130.5428751474886359No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT26480.38716223823051654No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA24260.35470377263868313No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC20260.2962200508515961No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT15690.2294023987098491No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT14510.2121497007826584No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG12800.18714790971867867No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT12660.18510097945613063No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT12100.17691325840593844No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA12080.176620839797003No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA11430.16711723500660136No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT11180.1634620023949084No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT10380.151765258037491TruSeq Adapter, Index 3 (95% over 21bp)
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG10040.1467941416855886No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC9480.13860642063539638No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT9210.134658769414768No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG8760.1280793507137207No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC8750.12793314140925302No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG8380.12252339714394744No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC8150.11916058314118995No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA8020.11725986218310962No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA7990.11682123426970646No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT7540.11024181556865917No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA7410.10834109461057882No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC6940.1014692573005961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7900.051.1758041
GTATCAA13000.044.636421
AACGCAG14950.037.8072436
CAACGCA15050.037.5587775
TCAACGC15100.037.434414
ATCAACG15300.036.9450723
CGCAGAG17500.032.2981878
ACGCAGA17650.032.0236977
TATCAAC19200.029.9354022
CATATAG2400.029.6880023
ACATGGG21950.028.3489993
GTACATG23200.028.292021
CTCGTCC4900.027.1413359
CTTAATA16600.026.6118972
GCAGAGT20800.026.4888979
TAATACT16850.026.217064
CCTCGTC5100.026.0769658
ACTTCAG16450.025.9863828
GCGTAAC559.4815434E-525.90763526-27
CATGGGA15250.025.8528984
Produced by FastQC (version 0.11.5)