##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031453_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683951
Sequences flagged as poor quality	0
Sequence length	101
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.34876182650512	34.0	33.0	34.0	31.0	34.0
2	33.438309177119415	34.0	34.0	34.0	31.0	34.0
3	33.53462894271666	34.0	34.0	34.0	31.0	34.0
4	36.70184121377116	37.0	37.0	37.0	35.0	37.0
5	36.63358339998041	37.0	37.0	37.0	35.0	37.0
6	36.67711429619958	37.0	37.0	37.0	37.0	37.0
7	36.6807344385782	37.0	37.0	37.0	37.0	37.0
8	36.68390718048515	37.0	37.0	37.0	37.0	37.0
9	38.631407805529925	39.0	39.0	39.0	38.0	39.0
10-11	38.62974686783117	39.0	39.0	39.0	38.5	39.0
12-13	38.63491682883715	39.0	39.0	39.0	39.0	39.0
14-15	40.36004772271698	41.0	41.0	41.0	39.0	41.0
16-17	40.41893425113787	41.0	41.0	41.0	39.0	41.0
18-19	40.45650492506042	41.0	41.0	41.0	39.5	41.0
20-21	40.454371000261716	41.0	41.0	41.0	39.5	41.0
22-23	40.415722763765245	41.0	41.0	41.0	39.0	41.0
24-25	40.39536019393202	41.0	41.0	41.0	39.0	41.0
26-27	40.33640202295194	41.0	41.0	41.0	39.0	41.0
28-29	40.282668641467005	41.0	40.5	41.0	39.0	41.0
30-31	40.165530133006605	41.0	40.0	41.0	39.0	41.0
32-33	40.086408236847376	41.0	40.0	41.0	38.0	41.0
34-35	40.055741566281796	41.0	40.0	41.0	38.0	41.0
36-37	39.99939688661907	41.0	40.0	41.0	38.0	41.0
38-39	39.901169089598525	41.0	40.0	41.0	38.0	41.0
40-41	39.84633548309748	41.0	40.0	41.0	38.0	41.0
42-43	39.716171187702045	41.0	40.0	41.0	38.0	41.0
44-45	39.632899871482024	41.0	40.0	41.0	38.0	41.0
46-47	39.554715176964436	41.0	40.0	41.0	37.5	41.0
48-49	39.432341644357564	41.0	40.0	41.0	37.0	41.0
50-51	39.38388422562436	41.0	40.0	41.0	37.0	41.0
52-53	39.44215813705952	41.0	40.0	41.0	37.0	41.0
54-55	39.42947374885043	41.0	40.0	41.0	37.0	41.0
56-57	39.40118224843593	41.0	40.0	41.0	37.0	41.0
58-59	39.41832382729172	41.0	40.0	41.0	37.0	41.0
60-61	39.317128712437	41.0	40.0	41.0	36.0	41.0
62-63	39.18311765024103	41.0	39.0	41.0	36.0	41.0
64-65	38.99375832479227	41.0	39.0	41.0	35.0	41.0
66-67	38.77686778731225	41.0	39.0	41.0	35.0	41.0
68-69	38.49124425580195	40.0	37.5	41.0	35.0	41.0
70-71	38.167134780123135	40.0	37.0	41.0	35.0	41.0
72-73	37.847573144859794	39.0	37.0	41.0	35.0	41.0
74-75	37.47123989876468	39.0	36.0	41.0	35.0	41.0
76-77	36.21465426616819	37.0	35.0	39.0	33.0	40.0
78-79	36.56449365524723	37.0	35.0	39.0	34.0	41.0
80-81	36.23144494269326	37.0	35.0	39.0	34.5	40.5
82-83	35.81947025444805	36.0	35.0	38.0	34.0	39.0
84-85	35.38753653404996	36.0	35.0	37.0	34.0	39.0
86-87	35.07350965200723	36.0	35.0	37.0	34.0	38.0
88-89	34.795883038404796	35.0	35.0	36.5	34.0	37.0
90-91	34.55984346831863	35.0	35.0	36.0	34.0	37.0
92-93	34.38923109988873	35.0	35.0	36.0	34.0	36.5
94-95	34.2478679028176	35.0	35.0	36.0	34.0	36.0
96-97	34.12386048123331	35.0	35.0	35.0	34.0	36.0
98-99	34.03857805603033	35.0	35.0	35.0	33.5	36.0
100-101	33.803861680149595	35.0	34.5	35.0	33.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.07359206790777506
1101	2	-0.10291580502117625
1101	3	-0.10692719130982908
1101	4	-0.08678165590470144
1101	5	-0.025972682891605814
1101	6	-0.07299318483985218
1101	7	-0.13527510845781876
1101	8	-0.10296023949894817
1101	9	0.026242570502624574
1101	10-11	-0.051346083122787434
1101	12-13	0.02530102622730368
1101	14-15	-0.08201641647702473
1101	16-17	-0.12215146568446755
1101	18-19	-0.08695005793137511
1101	20-21	-0.027534247141275614
1101	22-23	-0.08671114314660144
1101	24-25	-0.0882912966535585
1101	26-27	-0.1116463415443647
1101	28-29	-0.046461345810058674
1101	30-31	-0.13222149027792796
1101	32-33	-0.13052600654030044
1101	34-35	-0.10850285969628715
1101	36-37	-0.07398379119305787
1101	38-39	-0.040071237320532305
1101	40-41	-0.034363510808049114
1101	42-43	0.16735232841691072
1101	44-45	0.09614068072977489
1101	46-47	0.04266121594374539
1101	48-49	0.132618105026026
1101	50-51	0.16153946603802893
1101	52-53	0.09113840902995918
1101	54-55	0.06315337038625302
1101	56-57	-0.004946682634418664
1101	58-59	-0.15383452737213332
1101	60-61	-0.05826359924436275
1101	62-63	-0.08219605949005881
1101	64-65	-0.14813150164637534
1101	66-67	-0.15246975437916177
1101	68-69	-0.1921196570506396
1101	70-71	-0.19634511601188365
1101	72-73	-0.16477854220284627
1101	74-75	-0.2354331806326826
1101	76-77	-0.29082304503183565
1101	78-79	-0.20921177607549168
1101	80-81	-0.24163021618905844
1101	82-83	-0.320094294910092
1101	84-85	-0.2613833452233578
1101	86-87	-0.1748590235824352
1101	88-89	-0.22157169176769287
1101	90-91	-0.19637727483667788
1101	92-93	-0.17746786925884095
1101	94-95	-0.17788951856480395
1101	96-97	-0.17424363090101735
1101	98-99	-0.09759627297890461
1101	100-101	0.030062667457194436
1102	1	0.09079519532627955
1102	2	0.1382447875946653
1102	3	0.09324481720924638
1102	4	0.05176365177844389
1102	5	0.17775281243191188
1102	6	0.1175527388197466
1102	7	0.07734427329940274
1102	8	0.06733109993022879
1102	9	0.05468208713544698
1102	10-11	0.04421888641252991
1102	12-13	0.0739166772592057
1102	14-15	0.1513413234705041
1102	16-17	0.08333624529473127
1102	18-19	0.024752332464046845
1102	20-21	-0.09576961987966826
1102	22-23	-0.0884225116893802
1102	24-25	0.015471680256048614
1102	26-27	0.005816300355398596
1102	28-29	0.05758928048109624
1102	30-31	0.09003995685996102
1102	32-33	0.14956749403644665
1102	34-35	0.0921543755573282
1102	36-37	0.11465609212008587
1102	38-39	0.1443309023259829
1102	40-41	0.020382849265047298
1102	42-43	0.04564032065328405
1102	44-45	-0.02475341490347205
1102	46-47	-0.07480723705463532
1102	48-49	-0.04629109365252759
1102	50-51	0.05510774208845248
1102	52-53	0.06718506052965978
1102	54-55	0.042628570070128546
1102	56-57	0.05152847927731585
1102	58-59	0.010843290792777793
1102	60-61	0.0382296932950581
1102	62-63	0.1253676664701473
1102	64-65	0.15913179051533177
1102	66-67	0.13154623776423335
1102	68-69	0.17235680654718522
1102	70-71	0.23610957921052034
1102	72-73	0.1056336680775658
1102	74-75	0.14269500639171895
1102	76-77	-0.007977989259806861
1102	78-79	0.19201407517782343
1102	80-81	0.3234439189015532
1102	82-83	0.2225068690529639
1102	84-85	0.15323563556805198
1102	86-87	0.12148376150280171
1102	88-89	0.12376591853421814
1102	90-91	0.1969803488888786
1102	92-93	0.21075710161301942
1102	94-95	0.09642339166244795
1102	96-97	0.12102555371468782
1102	98-99	0.14436334569680298
1102	100-101	0.15273350649267314
1103	1	0.008053617186185136
1103	2	0.0704892913724322
1103	3	0.026372693139485648
1103	4	-7.359221578226993E-4
1103	5	-0.012024498253765614
1103	6	0.006472241177299054
1103	7	-0.011362197188589107
1103	8	0.06310738806293159
1103	9	0.014708787129762868
1103	10-11	0.03462819164459319
1103	12-13	0.10700904787785248
1103	14-15	0.13461832170943921
1103	16-17	0.05259006566625857
1103	18-19	0.08615095769837922
1103	20-21	0.11242500603637495
1103	22-23	0.09335332197533575
1103	24-25	0.06993256760478772
1103	26-27	0.03895269756042552
1103	28-29	0.05750690815819581
1103	30-31	0.12550976314297912
1103	32-33	0.1961859681601723
1103	34-35	0.10112585769105209
1103	36-37	0.17224712773901274
1103	38-39	-0.07582049232265575
1103	40-41	0.20417112399094606
1103	42-43	0.34494157612110143
1103	44-45	0.2977796760469289
1103	46-47	0.3057670968657149
1103	48-49	0.28146411852721087
1103	50-51	0.38221391717224407
1103	52-53	0.512129104158582
1103	54-55	0.38358607731321115
1103	56-57	0.3003397614451302
1103	58-59	0.39515136328042644
1103	60-61	0.48964280469652977
1103	62-63	0.4236179647147722
1103	64-65	0.3865717155281061
1103	66-67	0.36221430570719093
1103	68-69	0.3401037814946335
1103	70-71	0.3786548839881192
1103	72-73	0.3115715763886513
1103	74-75	0.3931792988418721
1103	76-77	0.16385067630855588
1103	78-79	0.29447672359002297
1103	80-81	0.2359559907074953
1103	82-83	0.35568871305682137
1103	84-85	0.3718389986311692
1103	86-87	0.30403929181013467
1103	88-89	0.38056447559836215
1103	90-91	0.3404970789916959
1103	92-93	0.32160468441140466
1103	94-95	0.4718778596508528
1103	96-97	0.5000007284008205
1103	98-99	0.3353478369767018
1103	100-101	0.2928857532687843
1104	1	0.0802795234638154
1104	2	0.07111926484723341
1104	3	0.05183688412091669
1104	4	0.03423313177675169
1104	5	0.027420683001757595
1104	6	-0.04684340869007286
1104	7	0.06942913791309735
1104	8	0.129928785056741
1104	9	0.06133787643126709
1104	10-11	0.1145574577807551
1104	12-13	0.17233840243135035
1104	14-15	0.12248856927796226
1104	16-17	0.09788736893769823
1104	18-19	0.029447818883170385
1104	20-21	0.06375679206642815
1104	22-23	0.06792781357569311
1104	24-25	0.11195290359064103
1104	26-27	0.14665053133584394
1104	28-29	0.0400389594402526
1104	30-31	0.18386520080876068
1104	32-33	0.10530953137857324
1104	34-35	0.026214275020848277
1104	36-37	0.0054533611607610055
1104	38-39	-0.008435434851399748
1104	40-41	0.05506634945513866
1104	42-43	0.20915466188591125
1104	44-45	0.09714970257212485
1104	46-47	0.04645564682150649
1104	48-49	0.11158127148919306
1104	50-51	0.035059849975077384
1104	52-53	0.09655660194815141
1104	54-55	0.2255677307172803
1104	56-57	0.11151902758128784
1104	58-59	0.0012411068701609906
1104	60-61	0.07349006375930145
1104	62-63	-0.04475626163358726
1104	64-65	0.02516587998434261
1104	66-67	0.07878702896095291
1104	68-69	0.03881067804636018
1104	70-71	0.20189206347529876
1104	72-73	0.1983421334178317
1104	74-75	0.3183671856676824
1104	76-77	0.1051968301547106
1104	78-79	0.13884564489859486
1104	80-81	0.21109414278529925
1104	82-83	0.14272452207539743
1104	84-85	0.2809404575171115
1104	86-87	0.31797607341932377
1104	88-89	0.35850987789720534
1104	90-91	0.18035671648064522
1104	92-93	0.3059934996191842
1104	94-95	0.198751201825921
1104	96-97	0.2447675276934831
1104	98-99	0.3097178635018949
1104	100-101	0.20954136776922638
1105	1	0.0037828168065630052
1105	2	0.0291732521443393
1105	3	0.01684511747777151
1105	4	0.016943082376606355
1105	5	-0.02740465221348387
1105	6	0.012730809141025645
1105	7	0.09145336750613353
1105	8	0.06462062227151222
1105	9	0.0764666696563907
1105	10-11	0.034899730187255784
1105	12-13	0.09163680280772724
1105	14-15	0.12168728722667765
1105	16-17	0.08003127625362794
1105	18-19	0.022927294300714607
1105	20-21	0.032139231492450904
1105	22-23	0.1609426924277244
1105	24-25	0.11536386571720669
1105	26-27	-0.04222414521085227
1105	28-29	0.010259201736175783
1105	30-31	0.04063419633922649
1105	32-33	0.10769867510945375
1105	34-35	0.06261747426808029
1105	36-37	0.12241848701350477
1105	38-39	0.14848352539325305
1105	40-41	0.20069384886704
1105	42-43	0.2943405005121207
1105	44-45	0.31799479784382356
1105	46-47	0.19960343557709592
1105	48-49	0.05566795697495763
1105	50-51	0.11222551687698257
1105	52-53	0.2096035320822338
1105	54-55	0.24205649806614105
1105	56-57	0.30795074952906987
1105	58-59	0.27810243361681586
1105	60-61	0.308927313818117
1105	62-63	0.16643669296532693
1105	64-65	0.1345813116474801
1105	66-67	0.20491176431733749
1105	68-69	0.07799263523290278
1105	70-71	0.0429515200737427
1105	72-73	0.008489745744341803
1105	74-75	0.027891415260725694
1105	76-77	-0.14287715243762023
1105	78-79	0.04074372770264745
1105	80-81	0.1551587746366252
1105	82-83	0.1121581847429951
1105	84-85	0.010658465044748766
1105	86-87	-0.020517810372716383
1105	88-89	-0.06169272149116978
1105	90-91	0.03188429822611738
1105	92-93	0.019274198278331767
1105	94-95	0.05438075957703603
1105	96-97	-0.016354855275231728
1105	98-99	0.06917152146473171
1105	100-101	0.0521813348358009
1106	1	0.07817541172855869
1106	2	0.09917010744725019
1106	3	0.07863385763920405
1106	4	0.0939220767115998
1106	5	0.14052445081864562
1106	6	-0.09576579777640148
1106	7	0.06287080553509128
1106	8	0.1288635934177762
1106	9	0.1521490461358752
1106	10-11	0.13706022823434694
1106	12-13	0.05901968562902482
1106	14-15	0.033758822249033926
1106	16-17	0.04148594931906757
1106	18-19	0.07279467011936447
1106	20-21	0.07889039480249238
1106	22-23	0.059338213285272445
1106	24-25	0.11984391967838093
1106	26-27	0.09070766955281329
1106	28-29	0.11957844318054356
1106	30-31	0.20067421147023623
1106	32-33	-0.03373977314204524
1106	34-35	0.03259587156637167
1106	36-37	0.13020616982750965
1106	38-39	0.16130500230707412
1106	40-41	0.19931861358445246
1106	42-43	0.3052273050897014
1106	44-45	0.19391257085138847
1106	46-47	-0.08131600092965385
1106	48-49	0.06577086604764304
1106	50-51	0.10107665420908774
1106	52-53	-0.23092295401500706
1106	54-55	0.07329906359205296
1106	56-57	0.18809221532251286
1106	58-59	0.2102407820583707
1106	60-61	0.20892822532451305
1106	62-63	0.1852809484241149
1106	64-65	0.19914558880012123
1106	66-67	0.27768529599446623
1106	68-69	0.12087103572147839
1106	70-71	0.38040600172283945
1106	72-73	0.37361870111242723
1106	74-75	0.26421198294691095
1106	76-77	0.24820239016846557
1106	78-79	0.2946848535042008
1106	80-81	0.2129152383563948
1106	82-83	0.261326207275431
1106	84-85	0.36328383135637665
1106	86-87	0.34911740476557185
1106	88-89	0.1933490482896616
1106	90-91	0.3902054401613313
1106	92-93	0.34070895492890685
1106	94-95	0.46431709085492656
1106	96-97	0.3123343529447311
1106	98-99	0.3232094948213984
1106	100-101	0.3810300566759466
1107	1	-0.08090012784741418
1107	2	-0.05070068263061245
1107	3	-0.14602393216095066
1107	4	-0.04932816231085724
1107	5	-0.08536290017655546
1107	6	-0.07548439785738026
1107	7	-0.0678442690971579
1107	8	-0.055097840759358974
1107	9	-0.049015645106464945
1107	10-11	0.02280325418444562
1107	12-13	0.05365519493234672
1107	14-15	0.0114454664585395
1107	16-17	-0.03312509595634339
1107	18-19	-0.1422396833964399
1107	20-21	-0.10281755049474839
1107	22-23	-0.06214773261827844
1107	24-25	0.03794772432230786
1107	26-27	-0.048318602778032016
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2316	32-33	-0.9468956343134423
2316	34-35	-0.8125546558533472
2316	36-37	-0.47677317205261005
2316	38-39	-0.35943446019252434
2316	40-41	-0.2980319411431509
2316	42-43	-0.2804802159068629
2316	44-45	-0.06484047586541664
2316	46-47	-0.06199858169851069
2316	48-49	-0.18296251318616896
2316	50-51	-0.08662724704096547
2316	52-53	-0.12829907356541526
2316	54-55	-0.17540599098012422
2316	56-57	-0.20558717274859362
2316	58-59	-0.1125971747312704
2316	60-61	-0.4409564424635164
2316	62-63	-0.46277714182552643
2316	64-65	-0.18421708597756492
2316	66-67	-0.13933506580587363
2316	68-69	-0.2273930256573422
2316	70-71	-0.10180320568049694
2316	72-73	-0.1432664285854699
2316	74-75	0.008510010678932645
2316	76-77	0.0474764073027103
2316	78-79	-0.06361630591861456
2316	80-81	0.08341851675441347
2316	82-83	0.07501384581208015
2316	84-85	0.15760393779900284
2316	86-87	0.1463142800470152
2316	88-89	0.08152401045082058
2316	90-91	0.03849230652412672
2316	92-93	0.09810532298101293
2316	94-95	0.011612013041997216
2316	96-97	-0.04526377385097646
2316	98-99	-0.21313976132940837
2316	100-101	-0.4300691435518118
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	17.0
15	46.0
16	151.0
17	275.0
18	486.0
19	726.0
20	967.0
21	1402.0
22	1903.0
23	1727.0
24	1447.0
25	1474.0
26	1668.0
27	1851.0
28	2762.0
29	3614.0
30	2903.0
31	3037.0
32	3701.0
33	5202.0
34	7405.0
35	12331.0
36	25011.0
37	73611.0
38	284398.0
39	244947.0
40	883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.16488666484202	25.530012191926982	15.059298429978382	22.245802713252612
2	11.788649981138862	24.665397560626133	44.13360119073728	19.412351267497726
3	13.952900134658769	33.22314025419949	33.570533561614795	19.25342604952694
4	9.518371930152892	21.074462936672365	45.70473615799962	23.70242897517512
5	9.448963956324166	42.4250089188067	38.173077485422866	9.952949639446274
6	22.49620221331645	45.9326764636648	21.01656405210315	10.554557270915607
7	21.824662877896223	35.11465002609836	28.856014539053238	14.20467255695218
8	21.302841870250937	39.709131209691925	24.562139685445302	14.425887234611837
9	24.826632317227403	16.660404034791966	24.29969398392575	34.21326966405488
10-11	19.70126514911156	29.506353525325647	34.16004947722863	16.632331848334164
12-13	20.95296300465969	27.84344200096206	32.759876072993535	18.44371892138472
14-15	19.521208390659563	29.535741595523657	29.465561129379154	21.47748888443763
16-17	18.04420199692668	33.24777652200231	30.15025930220147	18.557762178869538
18-19	18.042356704894292	31.796186595255797	31.703340768310184	18.45811593153973
20-21	19.04261992107368	31.673024539096893	31.086413940628336	18.197941599201087
22-23	19.082068744690776	31.260792074286027	30.91632295296008	18.74081622806312
24-25	19.148009141005716	31.961280851990857	30.105007522468714	18.78570248453471
26-27	18.605062597094033	31.846988942703096	31.082518504980357	18.46542995522252
28-29	18.884466869702653	30.536763598561883	31.461829867929136	19.116939663806328
30-31	19.505271576472584	31.26291210920081	30.59166519238951	18.640151121937098
32-33	19.19033673464912	31.211080910767	31.03365591979542	18.564926434788457
34-35	19.152541629444215	31.207352573503073	31.385874134258156	18.254231662794556
36-37	19.206657996182354	30.89770217544352	30.236961558172297	19.658678270201825
38-39	18.993106070021273	31.281408320966175	30.941536841951354	18.783948767061197
40-41	18.634741377671794	30.76389975305249	32.205450390451944	18.395908478823774
42-43	19.06262290719657	30.57061105254616	30.660091146880404	19.706674893376867
44-45	18.9560363242396	30.989646919150644	30.587205808603247	19.467110948006507
46-47	19.879866485820514	30.358170431654415	30.46236413689225	19.29959894563282
48-49	18.94069655232915	30.343670497412056	30.62805216831886	20.087580781939938
50-51	18.62750401710064	30.704027042872955	30.510957656323335	20.157511283703073
52-53	18.67677655270626	30.20187118667858	31.476743216984843	19.644609043630318
54-55	18.959691556851293	30.827062172582536	30.779471043978297	19.43377522658787
56-57	19.789794882966763	30.54670583126569	29.940229636333598	19.72326964943395
58-59	18.575526609362367	30.273075117954356	30.788389811550825	20.363008461132452
60-61	19.943853287470436	30.909861863003485	30.080674345411946	19.065610504114137
62-63	19.154746281315003	31.33536342400503	30.80522461990634	18.704665674773626
64-65	18.952486998939154	31.262205058880383	30.40760418578229	19.377703756398173
66-67	19.25473467194508	31.84631187241151	29.77230440806091	19.126649047582493
68-69	19.330687333635023	31.096588736638715	30.31258521578739	19.260138713938872
70-71	19.457075433076394	30.635183582281588	30.541462458914765	19.36627852572725
72-73	19.15142334744533	31.19374909987594	30.05050929659097	19.604318256087762
74-75	19.063396004614447	30.842297339382608	29.480553090260976	20.613753565741973
76-77	18.722845535647053	31.271196291457738	30.83884494787147	19.16711322502374
78-79	19.065327779329223	30.72771295019672	30.021741323574346	20.185217946899705
80-81	19.338446760074916	31.160565596073404	29.79818729704321	19.70280034680847
82-83	19.349558667214463	30.901628917861075	29.954923671432603	19.79388874349186
84-85	19.879640500562175	30.37271675894911	29.60701863145167	20.14062410903705
86-87	18.31522879414923	31.6835649676253	30.951414917438473	19.049791320786994
88-89	19.510522587356814	30.182101037600876	30.484177189741402	19.82319918530091
90-91	19.544186828571114	30.675531934336586	30.431432466910596	19.348848770181704
92-93	19.825469953256885	30.978388802706625	30.000687183731	19.19545406030549
94-95	19.829944655246784	30.005921961704647	29.927474246777646	20.23665913627092
96-97	19.6052787407285	30.46702176033078	30.424401748078445	19.50329775086227
98-99	19.614636136214436	30.138416348539586	30.622149832371033	19.62479768287494
100-101	19.27316430563008	30.687724705424802	30.778374474194788	19.260736514750327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22.0
1	13.5
2	10.0
3	12.0
4	10.0
5	13.0
6	15.5
7	31.0
8	59.5
9	74.5
10	73.0
11	68.0
12	64.5
13	73.5
14	87.5
15	84.5
16	106.0
17	133.5
18	146.0
19	164.0
20	198.0
21	260.5
22	295.0
23	335.0
24	398.5
25	601.0
26	1074.0
27	2146.5
28	4103.0
29	7849.0
30	13329.0
31	18841.0
32	26757.0
33	35452.5
34	40642.0
35	48325.5
36	62877.0
37	71043.5
38	63174.5
39	51763.5
40	41138.5
41	31556.5
42	25023.5
43	19851.5
44	16279.5
45	13051.5
46	10288.5
47	7936.5
48	6784.5
49	6223.5
50	5794.5
51	5365.0
52	4991.0
53	4727.5
54	4263.0
55	3963.0
56	3761.0
57	3403.5
58	3021.5
59	2543.5
60	2057.5
61	1728.0
62	1471.0
63	1287.0
64	1120.5
65	928.0
66	786.0
67	704.5
68	605.0
69	483.5
70	399.0
71	341.5
72	312.5
73	266.0
74	202.5
75	150.0
76	110.0
77	92.0
78	76.0
79	48.5
80	36.5
81	24.0
82	14.5
83	9.5
84	4.5
85	3.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10410102478101502
2	7.310465223385886E-4
3	0.0
4	0.0
5	4.3862791340315316E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0031435000460559312
20-21	0.0032166046982897894
22-23	0.0
24-25	0.0
26-27	0.001973825610314189
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0031435000460559312
38-39	0.0023393488714834832
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.018787895624101728
48-49	0.0016083023491448947
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0041669651773299544
62-63	0.010746383878377253
64-65	0.009430500138167793
66-67	0.0048980116996685434
68-69	0.003728337263926802
70-71	0.001023465131274024
72-73	0.0025586628281850598
74-75	0.0017545116536126126
76-77	0.0024124535237173427
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	9.503604790401652E-4
88-89	0.0025586628281850598
90-91	0.0012427790879756006
92-93	0.0
94-95	0.008187721050192193
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	683951.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.332387846426606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.01498089858	20.68406985188753
2	14.310610795693155	8.967712155398353
3	6.536402838380992	6.14403326557911
4	3.4301347731775214	4.298972523148506
5	2.0749696295066298	3.2506876600628924
6	1.3703234758970408	2.5761303973121708
7	0.9429276273563972	2.068092189200902
8	0.7140644181758425	1.7898674638094765
9	0.5448870684927413	1.5365351666265294
>10	3.4874122546220536	20.69052517633143
>50	0.3154966244459297	6.861142910412463
>100	0.23224714032075905	13.774157513023807
>500	0.01892033729700685	3.93454200189107
>1k	0.006622118053952396	3.4235317253157946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	3713	0.5428751474886359	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	2648	0.38716223823051654	No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA	2426	0.35470377263868313	No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC	2026	0.2962200508515961	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	1569	0.2294023987098491	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	1451	0.2121497007826584	No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG	1280	0.18714790971867867	No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT	1266	0.18510097945613063	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	1210	0.17691325840593844	No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA	1208	0.176620839797003	No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA	1143	0.16711723500660136	No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT	1118	0.1634620023949084	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	1038	0.151765258037491	TruSeq Adapter, Index 3 (95% over 21bp)
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG	1004	0.1467941416855886	No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC	948	0.13860642063539638	No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT	921	0.134658769414768	No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG	876	0.1280793507137207	No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC	875	0.12793314140925302	No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG	838	0.12252339714394744	No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC	815	0.11916058314118995	No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA	802	0.11725986218310962	No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA	799	0.11682123426970646	No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT	754	0.11024181556865917	No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA	741	0.10834109461057882	No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC	694	0.1014692573005961	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.4620930446771773E-4	0.0
8	0.0	0.0	0.0	1.4620930446771773E-4	0.0
9	0.0	0.0	0.0	1.4620930446771773E-4	0.0
10-11	0.0	0.0	0.0	1.4620930446771773E-4	0.0
12-13	0.0	0.0	0.0	1.4620930446771773E-4	0.0
14-15	0.0	0.0	0.0	2.1931395670157658E-4	0.0
16-17	0.0	0.0	0.0	3.655232611692943E-4	0.0
18-19	0.0	0.0	0.0	5.11732565637012E-4	0.0
20-21	0.0	0.0	0.0	7.310465223385886E-4	0.0
22-23	0.0	0.0	0.0	0.0012427790879756006	0.0
24-25	0.0	0.0	0.0	0.0027048721326527777	0.0
26-27	0.0	0.0	0.0	0.00445938378626539	0.0
28-29	0.0	0.0	0.0	0.009503604790401653	0.0
30-31	0.0	0.0	0.0	0.018861000276335586	0.0
32-33	0.0	0.0	0.0	0.03662543076916329	0.0
34-35	0.0	0.0	0.0	0.06491693118366666	0.0
36-37	0.0	0.0	0.0	0.103077559649741	0.0
38-39	0.0	0.0	0.0	0.16404683961277927	0.0
40-41	0.0	0.0	0.0	0.23071828245005854	0.0
42-43	0.0	0.0	0.0	0.29168756241309685	0.0
44-45	0.0	0.0	0.0	0.3601866215562226	0.0
46-47	0.0	0.0	0.0	0.44330661114612013	0.0
48-49	0.0	0.0	0.0	0.521601693688583	0.0
50-51	0.0	0.0	0.0	0.6136404508510113	0.0
52-53	0.0	0.0	0.0	0.6928858938725142	0.0
54-55	0.0	0.0	0.0	0.7703037205881709	0.0
56-57	0.0	0.0	0.0	0.8594182916612447	0.0
58-59	0.0	0.0	0.0	0.9554047000443013	0.0
60-61	0.0	0.0	0.0	1.059651934129784	0.0
62-63	0.0	0.0	0.0	1.1583432146454937	0.0
64-65	0.0	0.0	0.0	1.2476039950230353	0.0
66-67	0.0	0.0	0.0	1.342055205709181	0.0
68-69	0.0	0.0	0.0	1.4452789746633896	0.0
70-71	0.0	0.0	0.0	1.5680947904162725	0.0
72-73	0.0	0.0	0.0	1.6860856991217208	0.0
74-75	0.0	0.0	0.0	1.8018103636079192	0.0
76-77	0.0	0.0	0.0	1.9168039815717792	0.0
78-79	0.0	0.0	0.0	2.0462723206779434	0.0
80-81	0.0	0.0	0.0	2.1910195321009835	0.0
82-83	0.0	0.0	0.0	2.3430041040951766	0.0
84-85	0.0	0.0	0.0	2.48628922247354	0.0
86-87	0.0	0.0	0.0	2.6210942011927756	0.0
88-89	0.0	0.0	0.0	2.768546284748469	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	790	0.0	51.175804	1
GTATCAA	1300	0.0	44.63642	1
AACGCAG	1495	0.0	37.807243	6
CAACGCA	1505	0.0	37.558777	5
TCAACGC	1510	0.0	37.43441	4
ATCAACG	1530	0.0	36.945072	3
CGCAGAG	1750	0.0	32.298187	8
ACGCAGA	1765	0.0	32.023697	7
TATCAAC	1920	0.0	29.935402	2
CATATAG	240	0.0	29.688002	3
ACATGGG	2195	0.0	28.348999	3
GTACATG	2320	0.0	28.29202	1
CTCGTCC	490	0.0	27.141335	9
CTTAATA	1660	0.0	26.611897	2
GCAGAGT	2080	0.0	26.488897	9
TAATACT	1685	0.0	26.21706	4
CCTCGTC	510	0.0	26.076965	8
ACTTCAG	1645	0.0	25.986382	8
GCGTAAC	55	9.4815434E-5	25.907635	26-27
CATGGGA	1525	0.0	25.852898	4
>>END_MODULE