FastQCFastQC Report
Fri 10 Feb 2017
SRR2031452_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031452_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398152
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25440.18195446560888945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.1344632057172611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14850.058.4951251
GTATCAA30700.052.2604751
TCAACGC39050.040.236264
ATCAACG39850.039.42853
CAACGCA40550.038.5137375
AACGCAG41050.038.263576
TATCAAC42300.037.3679242
ACGCAGA47950.032.6584787
GTGCGTA600.003970122431.6358419
CGCAGAG50800.030.8262638
GTACATG53200.030.3362791
TACATGG54850.029.0775432
ACATGGG55450.027.7366523
GCAGAGT57900.026.390529
CATGGGG36600.025.5502854
TACACCG1501.0664244E-625.316835
AGAGTAC50900.022.28102710-11
GAGTACT26950.021.83323112-13
CAGAGTA53850.021.14855210-11
GCGCTAA600.004768324619.78940422-23
Produced by FastQC (version 0.11.5)