FastQCFastQC Report
Fri 10 Feb 2017
SRR2031452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398152
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32440.23202055284403983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.1771624258306679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14140.10113349621500381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.062.655851
GTATCAA36400.044.5446241
TCAACGC44500.036.5015374
ATCAACG44700.036.338223
CAACGCA45350.036.1315735
AACGCAG46050.035.4792026
TATCAAC46300.035.186312
ACGCAGA52300.031.1485237
GTACATG55500.030.9283371
TACATGG55500.029.9526792
CGCAGAG55250.029.4853928
ACATGGG56300.029.1042043
GCAGAGT63750.024.5854879
CATGGGG38550.024.517364
GTATTAC3000.023.7745511
GAGTACT32100.022.63846612-13
CAGAGTA60350.021.72085210-11
TATACCG1100.00287954221.5884955
AGAGTAC54600.020.83329410-11
CATGGGA18800.020.2105064
Produced by FastQC (version 0.11.5)