FastQCFastQC Report
Fri 10 Feb 2017
SRR2031451_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031451_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522068
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.11490945214011462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.055.7721561
GTATCAA25800.044.3311961
TCAACGC30650.036.546924
CAACGCA31300.035.93965
ATCAACG31600.035.44823
AACGCAG33200.033.869456
TATCAAC34700.032.6895642
ACGCAGA36750.030.5977067
CGCAGAG38650.029.2163058
GTACATG44650.027.5291
TACATGG44750.026.5147572
ACATGGG46150.025.095013
GCAGAGT47700.023.1750749
CATGGGG32150.022.7357354
AGAGTAC39950.021.6134410-11
GTATAAT10050.019.8333171
GAGTACA32900.019.3295171
CAGAGTA46150.019.12099810-11
GTATATA8950.019.0893691
GAGTACT24050.019.03625712-13
Produced by FastQC (version 0.11.5)