FastQCFastQC Report
Fri 10 Feb 2017
SRR2031451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522068
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22160.145591392763004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.10282063613452225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.058.4120061
GTATCAA31500.042.4223671
TCAACGC35600.036.2890474
ATCAACG36600.035.2975433
CAACGCA37000.034.9159475
AACGCAG38500.033.8023226
TATCAAC40200.032.9636152
ACGCAGA42500.030.3974137
GTACATG46500.029.5558871
CGCAGAG45300.028.4136988
TACATGG47900.027.962132
ACATGGG48500.026.7348383
GCAGAGT52850.023.8153829
CATGGGG37250.023.206084
TATACTG7300.021.4707765
AGAGTAC45900.021.10931410-11
CAGAGTA50550.020.71782310-11
GAGTACT28200.020.12716512-13
GTATAAT12100.019.257941
ATTATAC7700.019.121753
Produced by FastQC (version 0.11.5)