FastQCFastQC Report
Fri 10 Feb 2017
SRR2031450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705439
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18650.2643743824767273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.18527470128529894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.11794074328184295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.055.4662171
GTATCAA19400.049.1839561
TCAACGC23750.040.1812324
CAACGCA23800.039.897335
ATCAACG24200.039.434063
AACGCAG24700.038.4299436
TATCAAC25850.037.281682
ACGCAGA29350.032.503097
CGCAGAG30300.031.3273818
GTACATG28850.028.959831
TACATGG29150.028.500962
GCAGAGT33050.028.1463019
ACATGGG29850.027.5164623
CATGGGG15600.024.0432574
AGAGTAC31200.022.51354210-11
CATGGGA11400.022.4894964
CAGAGTA32100.022.10410110-11
ATGGGGG7350.021.3165725
GTATAAG3151.4551915E-1121.098261
TGTTCGC1150.003739632220.6352088
Produced by FastQC (version 0.11.5)