FastQCFastQC Report
Fri 10 Feb 2017
SRR2031450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705439
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.31597345766253354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15220.21575217701317903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10100.14317325807050646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9300.069.0228961
GTATCAA21500.046.664471
TCAACGC26550.037.5668074
CAACGCA26900.037.2545855
AACGCAG27750.036.2846076
ATCAACG27850.035.983783
TATCAAC28800.034.63192
ACGCAGA30600.032.594737
CGCAGAG31550.031.6132748
GCAGAGT36700.026.2711759
GTACATG28000.025.9821911
TACATGG29150.024.2771242
GTTAGAC1652.4298133E-623.0279663
AGAGTAC33000.022.74011610-11
CAGAGTA34350.022.53774310-11
ACATGGG30350.022.534783
GAGTACT20900.021.02357512-13
CATGGGG18250.020.8198054
AGTACTT22500.020.37304512-13
GTACTTT21800.020.15848714-15
Produced by FastQC (version 0.11.5)