##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	705439
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.29309408751146	34.0	33.0	34.0	31.0	34.0
2	33.3945032809357	34.0	34.0	34.0	31.0	34.0
3	33.48249954992565	34.0	34.0	34.0	31.0	34.0
4	36.65888900386852	37.0	37.0	37.0	35.0	37.0
5	36.56887555125248	37.0	37.0	37.0	35.0	37.0
6	36.61488378158849	37.0	37.0	37.0	37.0	37.0
7	36.62658429715397	37.0	37.0	37.0	37.0	37.0
8	36.63072923385296	37.0	37.0	37.0	37.0	37.0
9	38.56830427577721	39.0	39.0	39.0	38.0	39.0
10-11	38.57374273324837	39.0	39.0	39.0	38.0	39.0
12-13	38.573754782482965	39.0	39.0	39.0	38.0	39.0
14-15	40.27924880818894	41.0	41.0	41.0	39.0	41.0
16-17	40.33865507861063	41.0	41.0	41.0	39.0	41.0
18-19	40.36528601339025	41.0	41.0	41.0	39.0	41.0
20-21	40.36097593129952	41.0	41.0	41.0	39.0	41.0
22-23	40.31753277037419	41.0	41.0	41.0	39.0	41.0
24-25	40.28772012888428	41.0	41.0	41.0	39.0	41.0
26-27	40.20696332354747	41.0	40.0	41.0	39.0	41.0
28-29	40.142972673753505	41.0	40.0	41.0	38.5	41.0
30-31	40.00547673151045	41.0	40.0	41.0	38.0	41.0
32-33	39.87894346640886	41.0	40.0	41.0	38.0	41.0
34-35	39.802935477057545	41.0	40.0	41.0	38.0	41.0
36-37	39.722358701461076	41.0	40.0	41.0	37.0	41.0
38-39	39.621823431933876	41.0	40.0	41.0	37.0	41.0
40-41	39.51850124532383	41.0	40.0	41.0	37.0	41.0
42-43	39.327627193846666	41.0	40.0	41.0	36.0	41.0
44-45	39.18764556538552	41.0	40.0	41.0	35.5	41.0
46-47	39.0234853757731	41.0	39.0	41.0	35.0	41.0
48-49	38.807210120223004	41.0	39.0	41.0	35.0	41.0
50-51	38.69418262953991	41.0	39.0	41.0	35.0	41.0
52-53	38.70094862915149	41.0	39.0	41.0	35.0	41.0
54-55	38.63416043059712	41.0	39.0	41.0	35.0	41.0
56-57	38.55063442764009	41.0	39.0	41.0	35.0	41.0
58-59	38.501540175692014	41.0	38.0	41.0	35.0	41.0
60-61	38.32078039348548	40.5	37.0	41.0	35.0	41.0
62-63	38.11525092885423	40.0	37.0	41.0	35.0	41.0
64-65	37.84809104685168	39.5	36.5	41.0	35.0	41.0
66-67	37.55965576045555	39.0	36.0	41.0	35.0	41.0
68-69	37.21590456439182	39.0	35.0	41.0	34.0	41.0
70-71	36.86954931609962	37.5	35.0	41.0	34.0	41.0
72-73	36.52508367130255	37.0	35.0	39.5	34.0	41.0
74-75	36.150909575455856	37.0	35.0	39.0	34.0	41.0
76-77	34.96665905911071	35.5	34.5	38.5	32.0	39.0
78-79	35.351211089832006	36.0	35.0	37.5	33.0	39.5
80-81	35.13432699354586	35.0	35.0	37.0	33.0	39.0
82-83	34.843112586630454	35.0	35.0	37.0	33.0	39.0
84-85	34.5627396557321	35.0	35.0	36.0	33.0	37.5
86-87	34.360213285627815	35.0	35.0	36.0	33.0	37.0
88-89	34.18179459882428	35.0	35.0	36.0	33.0	37.0
90-91	34.027267417877376	35.0	35.0	35.0	33.0	36.0
92-93	33.92534294247979	35.0	35.0	35.0	33.0	36.0
94-95	33.83362062488748	35.0	35.0	35.0	33.0	36.0
96-97	33.76295966058015	35.0	35.0	35.0	33.0	36.0
98-99	33.70165599009978	35.0	35.0	35.0	32.5	36.0
100-101	33.44724632462906	35.0	34.5	35.0	31.5	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10077272279753657
1101	2	0.07326911595384189
1101	3	0.06585857550298613
1101	4	0.10421510059450156
1101	5	0.021825964296027678
1101	6	0.02892892181186113
1101	7	0.08442174503621658
1101	8	0.13114596324466277
1101	9	0.09277547527707952
1101	10-11	0.09139824615655812
1101	12-13	0.12874730982230886
1101	14-15	0.12470537509431523
1101	16-17	0.16305565800370658
1101	18-19	0.10350578089551021
1101	20-21	0.16625362805940824
1101	22-23	0.1723916426896679
1101	24-25	0.07224926552998312
1101	26-27	0.1446837901665461
1101	28-29	0.2341557885268486
1101	30-31	0.27793655400979844
1101	32-33	0.21896407000463114
1101	34-35	0.18686156548753985
1101	36-37	0.21060834362773306
1101	38-39	0.25772953199464155
1101	40-41	0.18897989251302505
1101	42-43	0.3063399026427476
1101	44-45	0.36097913669218684
1101	46-47	0.27456333778022923
1101	48-49	0.2736313482572328
1101	50-51	0.30025263015535586
1101	52-53	0.3644664725235671
1101	54-55	0.40077444828774134
1101	56-57	0.32533674234451126
1101	58-59	0.34906585512955246
1101	60-61	0.25963076727968826
1101	62-63	0.28780585662990177
1101	64-65	0.42624912694379447
1101	66-67	0.31476021726270886
1101	68-69	0.23184200490151596
1101	70-71	0.20083614772753577
1101	72-73	0.2913800997064939
1101	74-75	0.25711703792298835
1101	76-77	0.3052287191233134
1101	78-79	0.3191211163754417
1101	80-81	0.25383233518128634
1101	82-83	0.24932088612418113
1101	84-85	0.23595297639226231
1101	86-87	0.3274967387855696
1101	88-89	0.2747717790439381
1101	90-91	0.344226856717917
1101	92-93	0.23964469911609854
1101	94-95	0.2431306486266962
1101	96-97	0.24822880249876533
1101	98-99	0.2866247789908769
1101	100-101	0.33221482713338446
1102	1	0.07413973942269081
1102	2	0.11736337462311752
1102	3	0.13628154287144412
1102	4	0.07548287765065709
1102	5	0.12062318440167985
1102	6	0.07696340968433901
1102	7	0.10475700970404489
1102	8	0.08059801313547865
1102	9	0.15753995645039964
1102	10-11	0.10434270452206817
1102	12-13	0.1661475256272027
1102	14-15	0.21031778540999824
1102	16-17	0.11689795678609727
1102	18-19	0.06665530505083694
1102	20-21	0.02538084555699527
1102	22-23	0.11903618338832445
1102	24-25	0.049213882731990566
1102	26-27	0.06352683045864893
1102	28-29	0.05532537946918126
1102	30-31	0.03717474227144635
1102	32-33	0.20708457300391814
1102	34-35	0.2465032373106837
1102	36-37	0.22860018190701936
1102	38-39	0.13641342896058717
1102	40-41	0.18534138105541587
1102	42-43	0.17006192343569637
1102	44-45	0.14903136965919828
1102	46-47	0.13437197713547278
1102	48-49	0.196481097933777
1102	50-51	0.20160371199245475
1102	52-53	0.285668280784499
1102	54-55	0.2988961274748405
1102	56-57	0.18050966752362285
1102	58-59	0.3430967019336464
1102	60-61	0.33245469196275224
1102	62-63	0.3946753048955358
1102	64-65	0.43289714506730803
1102	66-67	0.4972316947080273
1102	68-69	0.5411794020490035
1102	70-71	0.5311454851246751
1102	72-73	0.5212877433931808
1102	74-75	0.5323219758663029
1102	76-77	0.25129155823616145
1102	78-79	0.42179208550307123
1102	80-81	0.45909500860506114
1102	82-83	0.40859205878343374
1102	84-85	0.4222445073285641
1102	86-87	0.36491357463577145
1102	88-89	0.29449210203534193
1102	90-91	0.26911703657237496
1102	92-93	0.34754586865590653
1102	94-95	0.27107533711591003
1102	96-97	0.20691776218620106
1102	98-99	0.24288209820313256
1102	100-101	0.3840450776613906
1103	1	0.07877482805018587
1103	2	0.06032829749945989
1103	3	0.05504047033014814
1103	4	-0.023771290094501296
1103	5	0.033222764511535274
1103	6	0.03837761500869874
1103	7	0.039218677952490566
1103	8	0.0660552428192247
1103	9	0.09834790943804705
1103	10-11	0.059355502543411376
1103	12-13	0.0975127437687604
1103	14-15	0.00999280194676544
1103	16-17	-0.012381231809769133
1103	18-19	0.03286757006094376
1103	20-21	0.001975325631391911
1103	22-23	0.004858180646571952
1103	24-25	-0.009680981762365093
1103	26-27	-0.04754273901016148
1103	28-29	0.07199396344240938
1103	30-31	0.1311874731870546
1103	32-33	0.11949961235662698
1103	34-35	0.05423813133155875
1103	36-37	0.06015761003199316
1103	38-39	-0.025994420492502
1103	40-41	0.08367859693455415
1103	42-43	0.13005492526672668
1103	44-45	0.1849161587075443
1103	46-47	-0.04782966599625382
1103	48-49	-0.018851602688386038
1103	50-51	0.07160074016727691
1103	52-53	0.10674762851680697
1103	54-55	0.10218526689178731
1103	56-57	-0.01725571167168738
1103	58-59	0.15474529798591163
1103	60-61	0.20078845669006284
1103	62-63	0.060649344564609464
1103	64-65	0.1280166411991459
1103	66-67	0.06345067029112528
1103	68-69	0.13668283882102372
1103	70-71	0.2262937408550343
1103	72-73	0.10746108707996171
1103	74-75	0.035821060352411394
1103	76-77	-0.08158401815412475
1103	78-79	0.14069097634121874
1103	80-81	0.04849661722527543
1103	82-83	0.12003742033952136
1103	84-85	0.12711590399053563
1103	86-87	0.07867408387240005
1103	88-89	0.0723769294298151
1103	90-91	0.1223783750441001
1103	92-93	-0.06534130743911959
1103	94-95	-0.0854334393549081
1103	96-97	-0.08648766187407375
1103	98-99	-0.11348048364188656
1103	100-101	-0.10893039040107766
1104	1	-0.054925109313948894
1104	2	-0.08518034256538698
1104	3	-0.11592320086840147
1104	4	-0.028970987968563122
1104	5	-0.042230424390858445
1104	6	-0.039387574188452845
1104	7	0.03807649453994344
1104	8	0.022306854771620976
1104	9	0.00387828783235733
1104	10-11	0.0218914260539691
1104	12-13	0.07832129909096608
1104	14-15	0.12293770080826505
1104	16-17	0.0615267855809023
1104	18-19	0.02820211926694327
1104	20-21	0.015216363176058678
1104	22-23	0.03371212854826666
1104	24-25	0.039476657970872964
1104	26-27	0.010003961553564977
1104	28-29	0.004349532507667675
1104	30-31	0.043725238928928434
1104	32-33	-0.014140452489911581
1104	34-35	-0.140570921393099
1104	36-37	-0.08156524468964932
1104	38-39	-0.047564646350068074
1104	40-41	0.017450381319804364
1104	42-43	0.15939936725486348
1104	44-45	0.05896971237174142
1104	46-47	0.14998222376461712
1104	48-49	0.09355214072564166
1104	50-51	0.1701117567105186
1104	52-53	0.08184987235453889
1104	54-55	0.14011680957629125
1104	56-57	0.285855817011857
1104	58-59	0.3129077643651854
1104	60-61	0.38734431314340156
1104	62-63	0.37276477877853154
1104	64-65	0.22090654945602495
1104	66-67	0.21974557467932243
1104	68-69	0.2140841873343433
1104	70-71	0.31656537786177097
1104	72-73	0.33428581719308426
1104	74-75	0.3319735602603018
1104	76-77	0.1717343009940251
1104	78-79	0.3677782244160852
1104	80-81	0.34945503806459755
1104	82-83	0.3217525376896546
1104	84-85	0.3682754964889696
1104	86-87	0.2800852330562833
1104	88-89	0.23435972298938168
1104	90-91	0.14730376467601758
1104	92-93	0.09352350962731037
1104	94-95	-0.01733535895993299
1104	96-97	0.18083852360177133
1104	98-99	0.07678746890223209
1104	100-101	-0.02197476975334922
1105	1	-0.05297508700247988
1105	2	0.01633237497142659
1105	3	0.011200850894198311
1105	4	0.08504284514165761
1105	5	0.005353243614493408
1105	6	0.0029172004657169737
1105	7	-0.017363041381521782
1105	8	-0.029948834652742562
1105	9	-0.005546076290805502
1105	10-11	0.04914823105173127
1105	12-13	0.03727489123732397
1105	14-15	0.11175630857265162
1105	16-17	0.14066667848585013
1105	18-19	0.05493348919108598
1105	20-21	0.07710614452366826
1105	22-23	0.0026121731712080987
1105	24-25	0.02364555578434846
1105	26-27	-5.158956530451064E-4
1105	28-29	0.022255601226987665
1105	30-31	0.002584007335883598
1105	32-33	0.10172523247215537
1105	34-35	0.11780591930301654
1105	36-37	0.1282785750336899
1105	38-39	0.1084092492322668
1105	40-41	0.11168063567053821
1105	42-43	0.10482216957525736
1105	44-45	0.09429434423251593
1105	46-47	0.1153983326445811
1105	48-49	0.20253473778052467
1105	50-51	0.37606557197155865
1105	52-53	0.3276106934165668
1105	54-55	0.20972859003143896
1105	56-57	0.21749794106361975
1105	58-59	0.20858491742185947
1105	60-61	0.2580973251915992
1105	62-63	0.14622528748000718
1105	64-65	0.21886951241898345
1105	66-67	0.23143232166727756
1105	68-69	0.33279904677210226
1105	70-71	0.23033043810273313
1105	72-73	0.3141787221462309
1105	74-75	0.3151618690510176
1105	76-77	0.21541903994985034
1105	78-79	0.46521017443839696
1105	80-81	0.5432468720672716
1105	82-83	0.5383656568329016
1105	84-85	0.5056684014421151
1105	86-87	0.42978179709020026
1105	88-89	0.2684047921549322
1105	90-91	0.41705387623336065
1105	92-93	0.44291217540151706
1105	94-95	0.3466494692417612
1105	96-97	0.35026735001854803
1105	98-99	0.47105230602851833
1105	100-101	0.42603393171921056
1106	1	0.01221009818270602
1106	2	0.0874434860825346
1106	3	0.07527492496827648
1106	4	0.03791321551202742
1106	5	0.10368657694782968
1106	6	-0.18634205879354226
1106	7	0.03837769935921642
1106	8	0.019495609791697177
1106	9	0.060287257042531905
1106	10-11	0.05437971253321905
1106	12-13	0.06070081716325859
1106	14-15	0.062311864128204775
1106	16-17	0.07983334515251528
1106	18-19	0.06335941511701293
1106	20-21	0.04020799637552841
1106	22-23	0.034649210208250736
1106	24-25	0.10021962985842237
1106	26-27	0.0367526228654782
1106	28-29	-0.005707361735971972
1106	30-31	0.2014728962247716
1106	32-33	0.033299306546233254
1106	34-35	0.13002814152523712
1106	36-37	0.19420450095961428
1106	38-39	0.09590924923226396
1106	40-41	0.22015285789276362
1106	42-43	0.31741476216785003
1106	44-45	0.2887387886769588
1106	46-47	-0.2249257414294945
1106	48-49	0.12878473778052069
1106	50-51	0.17245446086045035
1106	52-53	-0.14507449176861797
1106	54-55	0.14375636780922463
1106	56-57	0.060738681804359373
1106	58-59	0.11437195445888904
1106	60-61	0.014486214080477566
1106	62-63	0.04136417636888723
1106	64-65	0.05007321612269067
1106	66-67	-0.02731767833272869
1106	68-69	-0.29400650878346113
1106	70-71	-0.04564178411948916
1106	72-73	-0.05568238896488964
1106	74-75	0.013541498680645248
1106	76-77	-0.20152540449459622
1106	78-79	0.0026175818458042954
1106	80-81	0.0279690942894959
1106	82-83	-0.0701065653893238
1106	84-85	0.1296961792198914
1106	86-87	0.030846611905019472
1106	88-89	-0.1809933559932162
1106	90-91	0.025340913270390786
1106	92-93	-0.011856343116996015
1106	94-95	-0.07899867890638745
1106	96-97	-0.040890057388857315
1106	98-99	-0.04566065693445154
1106	100-101	0.019089487274762007
1107	1	-0.09890101292840114
1107	2	-0.07454885108221276
1107	3	-0.032119711047052135
1107	4	0.005451529688272672
1107	5	-0.10928277171117884
1107	6	-0.09833439595829674
1107	7	-0.040760231675264436
1107	8	-2.17033886464435E-4
1107	9	-0.007538413455556281
1107	10-11	0.03829254778226243
1107	12-13	0.05768357578394756
1107	14-15	0.028844431178015384
1107	16-17	0.05062836430960971
1107	18-19	0.041702326994403904
1107	20-21	0.01669745997705263
1107	22-23	0.05627756269866069
1107	24-25	0.0940414689388902
1107	26-27	0.03907541980034779
1107	28-29	0.0704037493751315
1107	30-31	0.10403994603320399
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2316	26-27	-0.23081925348557775
2316	28-29	-0.229907534671554
2316	30-31	-0.3580169438098153
2316	32-33	-0.4722940786677796
2316	34-35	-0.4298183781461873
2316	36-37	-0.2682360524342471
2316	38-39	-0.38685663922428404
2316	40-41	-0.36883600937918004
2316	42-43	-0.3525052551257062
2316	44-45	-0.19971776125819218
2316	46-47	-0.25329528315020156
2316	48-49	-0.22440646412177045
2316	50-51	-0.3360031699220798
2316	52-53	-0.167183225015485
2316	54-55	-0.22293461792359182
2316	56-57	-0.1495023471623469
2316	58-59	-0.17896732353505485
2316	60-61	-0.47279761644264795
2316	62-63	-0.37792084308635765
2316	64-65	-0.2688794427618788
2316	66-67	-0.15331476004478617
2316	68-69	-0.32542404012803416
2316	70-71	-0.3085606124809246
2316	72-73	-0.30122119138598435
2316	74-75	-0.3372988601606792
2316	76-77	-0.20385463060787146
2316	78-79	-0.28052119895834693
2316	80-81	-0.12457630130064956
2316	82-83	-0.08306322340921213
2316	84-85	-0.007260694969474457
2316	86-87	-0.06883183599813947
2316	88-89	-0.09939748483022726
2316	90-91	-0.12413217362973938
2316	92-93	-0.17099760987013468
2316	94-95	-0.11663918906635473
2316	96-97	-0.12163205911821962
2316	98-99	-0.5317312362686479
2316	100-101	-0.572625817524198
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	12.0
15	21.0
16	103.0
17	243.0
18	541.0
19	919.0
20	1482.0
21	2171.0
22	3254.0
23	3870.0
24	3502.0
25	2739.0
26	2385.0
27	2116.0
28	2208.0
29	2783.0
30	3235.0
31	4005.0
32	5135.0
33	6974.0
34	10902.0
35	19485.0
36	46442.0
37	142490.0
38	296703.0
39	141368.0
40	350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49122291704802	19.39911995039256	13.732292939311769	23.377364193247654
2	14.485004245619356	21.2516284324663	41.317176826147914	22.94619049576643
3	17.200211499505983	27.738755583402675	30.906144967885247	24.154887949206095
4	11.581157265192314	17.95421007344363	40.48443593280213	29.980196728561932
5	12.408354549526818	39.1477043984146	35.62123282622378	12.822708225834804
6	28.5274843041	39.327992923555406	19.211583141845008	12.93293963049959
7	25.874809870165954	33.731619601411325	23.687094135708403	16.706476392714322
8	25.45507123932757	35.375418710902004	21.358614990098364	17.810895059672063
9	26.836055279053184	15.626156194936769	20.624178702906985	36.91360982310306
10-11	23.598992967499672	26.639298365982032	29.985087300248498	19.776621366269797
12-13	25.152635451116257	24.449456296008584	29.47774364615509	20.92016460672007
14-15	22.260252126689906	25.51007245133881	27.341201719780166	24.88847370219112
16-17	21.478044168241336	28.784062123018433	27.57963480896293	22.1582588997773
18-19	21.471783410912384	27.833620046228468	29.613082897239124	21.081513645620024
20-21	22.62334007874769	26.918597851626874	29.50493856475066	20.953123504874775
22-23	22.28250777175631	26.799553186030263	29.254620172686796	21.663318869526634
24-25	21.990845416825554	27.04181367914164	29.271418223262398	21.695922680770412
26-27	21.838891448417623	27.14944891377538	29.214870468157493	21.796789169649504
28-29	21.82435334593069	27.05669802775293	29.413953580678132	21.70499504563825
30-31	22.2394140386341	26.908138053042148	29.140081566230393	21.712366342093365
32-33	21.90224810366311	27.179174953468692	29.10854092274456	21.81003602012364
34-35	22.11509428880456	27.144373928858485	29.06551806747288	21.67501371486408
36-37	22.248022454707833	27.004904879362652	28.994854129454794	21.752218536474725
38-39	22.09172517179421	27.20022397924648	28.86610506469527	21.84194578426404
40-41	22.10042257374486	27.06931428514726	29.010304221910044	21.81995891919783
42-43	22.080789409148064	27.062368255795327	28.87960546553281	21.9772368695238
44-45	22.06831490745479	27.210006818449223	28.72133522529942	22.000343048796562
46-47	22.303227768550045	27.25508169201398	28.374248459701278	22.067442079734693
48-49	22.32161842631261	27.49792324070845	28.15291752784842	22.027540805130517
50-51	22.355582835652694	27.634423387422586	27.909571203179862	22.10042257374486
52-53	22.42206625944979	27.5788551526071	27.936079519278067	22.062999068665043
54-55	22.354023522941034	27.553835271370026	27.996113058676936	22.096028147012003
56-57	22.48380086726138	27.52640554321493	27.836708772835074	22.153084816688615
58-59	22.43397373833882	27.46112704287685	27.92842471142083	22.1764745073635
60-61	22.39119432839672	27.600370848684946	28.085904772975677	21.922530049942658
62-63	22.20584003073551	27.660826257880583	28.071247410944277	22.062086300439628
64-65	22.30904728319233	27.506831448408832	28.058238319197876	22.12588294920096
66-67	22.392707178561	27.481407870077657	27.884650825198538	22.241234126162805
68-69	22.31663406197726	27.570241841739673	27.970216325026225	22.14290777125684
70-71	22.453262681590854	27.46898354483494	27.791835357624834	22.285918415949375
72-73	22.356515168912836	27.606273115563283	27.720247284253563	22.316964431270318
74-75	22.281942272770443	27.760018485415095	27.72223962711629	22.235799614698173
76-77	22.32655722831788	27.63715233477928	27.763105667545574	22.27318476935726
78-79	22.364513444819465	27.53547790808277	27.742015964527056	22.357992682570714
80-81	22.397329889614838	27.847694839667213	27.4872809697224	22.26769430099555
82-83	22.51002567195746	27.657600444545878	27.568648742130787	22.26372514136587
84-85	22.48287945520449	27.678651166153273	27.50188180693157	22.336587571710666
86-87	22.480581144555384	27.619817828844734	27.62350349535854	22.27609753124134
88-89	22.329880319950103	27.69092281575935	27.626635101658938	22.352561762631616
90-91	22.468296112747613	27.7093959179739	27.592730149099705	22.229577820178783
92-93	22.45254373517767	27.679147311106984	27.54589695211067	22.322412001604675
94-95	22.333068700558563	27.600654966117556	27.667994556125773	22.398281777198108
96-97	22.46565613752571	27.566309773063296	27.67000406838862	22.29803002102237
98-99	22.46593964892783	27.50457516525171	27.561631834928317	22.46785335089214
100-101	22.384926265772094	27.595298813930047	27.666601931563186	22.353172988734674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	12.5
2	32.0
3	55.0
4	49.0
5	43.5
6	81.0
7	190.0
8	367.5
9	459.5
10	405.5
11	375.5
12	399.0
13	459.0
14	521.0
15	565.5
16	618.0
17	670.0
18	713.0
19	762.5
20	881.5
21	1084.0
22	1352.5
23	1792.0
24	2321.5
25	2999.5
26	4086.5
27	5222.5
28	6363.5
29	8021.0
30	10029.5
31	11675.0
32	13349.0
33	15217.0
34	16494.0
35	17917.0
36	19969.0
37	21141.0
38	21728.5
39	22914.0
40	23618.0
41	23569.0
42	23889.0
43	24803.5
44	25382.0
45	25731.0
46	25816.5
47	25708.0
48	26034.5
49	25969.5
50	25277.5
51	24208.0
52	23084.0
53	22508.5
54	21671.0
55	20014.5
56	18134.0
57	16278.5
58	14861.0
59	13248.5
60	11041.0
61	8956.5
62	7114.5
63	5249.0
64	3652.0
65	2565.0
66	1719.0
67	1184.0
68	874.0
69	602.5
70	423.5
71	305.5
72	200.0
73	130.0
74	91.0
75	72.0
76	48.0
77	28.0
78	11.5
79	6.0
80	5.0
81	3.5
82	2.0
83	0.5
84	0.5
85	0.5
86	0.0
87	0.0
88	1.0
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10007952494829461
2	8.505342063594442E-4
3	0.0
4	0.0
5	4.252671031797221E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.004181793181267268
20-21	0.003047747572788009
22-23	0.0
24-25	0.0
26-27	0.0019845798148387032
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.00269335832013824
38-39	0.002338969067488472
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.019491408895737264
48-49	0.0018428241137787961
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0042526710317972215
62-63	0.009355876269953888
64-65	0.008717975615184304
66-67	0.004677938134976944
68-69	0.00269335832013824
70-71	9.922899074193516E-4
72-73	0.002268091216958518
74-75	0.002551602619078333
76-77	0.00269335832013824
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	4.961449537096758E-4
88-89	0.002338969067488472
90-91	7.087785052995369E-4
92-93	0.0
94-95	0.008363586362534536
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	705439.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.47163854605316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6832888327833	49.55108930436529
2	14.44272318754849	18.91177504311751
3	4.957262078917953	9.736802130269167
4	2.0448523644800938	5.355193395491313
5	1.04611399992366	3.424539884048387
6	0.5685669986813146	2.2335007816126384
7	0.33593243099820563	1.5395832688748026
8	0.23061888781344303	1.2079197171851621
9	0.14435127980219714	0.8505823333382672
>10	0.5265223972848726	5.4246365985576785
>50	0.014336998292351005	0.6384326363315818
>100	0.004778878258065079	0.4488420018013835
>500	0.0	0.0
>1k	6.516652160373469E-4	0.6771029050068589
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2229	0.31597345766253354	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1522	0.21575217701317903	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1010	0.14317325807050646	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	1.4175570105990737E-4
16-17	0.0	0.0	0.0	2.1263355158986105E-4	1.4175570105990737E-4
18-19	0.0	0.0	0.0	3.5438925264976844E-4	1.4175570105990737E-4
20-21	0.0	0.0	0.0	4.2526710317972215E-4	1.4175570105990737E-4
22-23	0.0	0.0	0.0	5.670228042396295E-4	1.4175570105990737E-4
24-25	0.0	0.0	0.0	7.087785052995369E-4	1.4175570105990737E-4
26-27	0.0	0.0	0.0	7.796563558294905E-4	1.4175570105990737E-4
28-29	0.0	0.0	0.0	8.505342063594443E-4	1.4175570105990737E-4
30-31	0.0	0.0	0.0	0.0013466791600691202	1.4175570105990737E-4
32-33	0.0	0.0	0.0	0.0028351140211981475	1.4175570105990737E-4
34-35	0.0	0.0	0.0	0.004323548882327175	1.4175570105990737E-4
36-37	0.0	0.0	0.0	0.006520762248755739	1.4175570105990737E-4
38-39	0.0	0.0	0.0	0.010560799728963101	1.4175570105990737E-4
40-41	0.0	0.0	0.0	0.015380493564999952	1.4175570105990737E-4
42-43	0.0	0.0	0.0	0.020129309550506846	1.4175570105990737E-4
44-45	0.0	0.0	0.0	0.025445148340253375	1.4175570105990737E-4
46-47	0.0	0.0	0.0	0.030831864980529856	2.1263355158986105E-4
48-49	0.0	0.0	0.0	0.03791965003352522	4.2526710317972215E-4
50-51	0.0	0.0	0.0	0.045078312937050544	4.2526710317972215E-4
52-53	0.0	0.0	0.0	0.05152819733527633	4.2526710317972215E-4
54-55	0.0	0.0	0.0	0.05826159313562193	4.2526710317972215E-4
56-57	0.0	0.0	0.0	0.06605815669391685	4.2526710317972215E-4
58-59	0.0	0.0	0.0	0.07449262090698133	4.2526710317972215E-4
60-61	0.0	0.0	0.0	0.08455727568223476	4.2526710317972215E-4
62-63	0.0	0.0	0.0	0.0942675412048384	4.2526710317972215E-4
64-65	0.0	0.0	0.0	0.10497009663486141	4.2526710317972215E-4
66-67	0.0	0.0	0.0	0.11567265206488442	4.2526710317972215E-4
68-69	0.0	0.0	0.0	0.12772188665497655	4.2526710317972215E-4
70-71	0.0	0.0	0.0	0.14090516685354793	5.670228042396295E-4
72-73	0.0	0.0	0.0	0.15423020275317922	7.087785052995369E-4
74-75	0.0	0.0	0.0	0.16904367351393956	7.087785052995369E-4
76-77	0.0	0.0	0.0	0.182156075861981	7.087785052995369E-4
78-79	0.0	0.0	0.0	0.19562286746267218	7.087785052995369E-4
80-81	0.0	0.0	0.0	0.21263355158986108	7.087785052995369E-4
82-83	0.0	0.0	0.0	0.23255022758877805	7.087785052995369E-4
84-85	0.0	0.0	0.0	0.2526086592887549	7.087785052995369E-4
86-87	0.0	0.0	0.0	0.27493518220569035	7.087785052995369E-4
88-89	0.0	0.0	0.0	0.29938804063852437	7.087785052995369E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	930	0.0	69.022896	1
GTATCAA	2150	0.0	46.66447	1
TCAACGC	2655	0.0	37.566807	4
CAACGCA	2690	0.0	37.254585	5
AACGCAG	2775	0.0	36.284607	6
ATCAACG	2785	0.0	35.98378	3
TATCAAC	2880	0.0	34.6319	2
ACGCAGA	3060	0.0	32.59473	7
CGCAGAG	3155	0.0	31.613274	8
GCAGAGT	3670	0.0	26.271175	9
GTACATG	2800	0.0	25.982191	1
TACATGG	2915	0.0	24.277124	2
GTTAGAC	165	2.4298133E-6	23.027966	3
AGAGTAC	3300	0.0	22.740116	10-11
CAGAGTA	3435	0.0	22.537743	10-11
ACATGGG	3035	0.0	22.53478	3
GAGTACT	2090	0.0	21.023575	12-13
CATGGGG	1825	0.0	20.819805	4
AGTACTT	2250	0.0	20.373045	12-13
GTACTTT	2180	0.0	20.158487	14-15
>>END_MODULE