FastQCFastQC Report
Fri 10 Feb 2017
SRR2031445_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031445_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784566
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.13650377738901223No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC18230.10215368890811548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.057.782511
GTATCAA37650.042.9808731
TCAACGC44050.036.424254
CAACGCA44700.035.8945925
ATCAACG45300.035.419173
AACGCAG46450.034.531616
TATCAAC49350.032.512432
GTACATG58100.030.792931
ACGCAGA53850.029.7863187
ACATGGG60200.028.466273
CGCAGAG56650.028.230328
TACATGG65650.026.4646532
CATGGGG39550.024.845274
GCAGAGT64100.024.7264679
TACCGTA1351.3530762E-524.6065167
AGAGTAC57350.021.71926710-11
GTACCGT1805.213611E-621.0912976
GAGTACT32750.020.71924812-13
CAGAGTA61450.020.46318410-11
GTATATA14150.020.122441
Produced by FastQC (version 0.11.5)