FastQCFastQC Report
Fri 10 Feb 2017
SRR2031445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784566
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31810.178250622280151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23790.13330972348458953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.061.3209881
GTATCAA40350.045.3612941
TCAACGC48000.037.1060144
ATCAACG49500.036.173493
CAACGCA49650.035.9685445
AACGCAG50900.035.2718546
TATCAAC53500.033.735262
ACGCAGA59400.030.1445777
CGCAGAG60300.029.3795978
GTACATG59550.029.1393131
ACATGGG61950.026.5270583
TACATGG65850.025.8936312
GCAGAGT71350.024.56339
CATGGGG40250.023.9543534
AGAGTAC64000.021.07621810-11
GAGTACT38500.020.84963812-13
CAGAGTA69900.020.75813510-11
GTATCGA1656.3296284E-520.149699
TATATAG14850.019.5100175
TATATAC11850.019.2387643
Produced by FastQC (version 0.11.5)