FastQCFastQC Report
Fri 10 Feb 2017
SRR2031444_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031444_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036840
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.17167547548319895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.14158404382546969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.062.0830841
GTATCAA23750.042.5569881
TATCAAC29250.034.074712
TCAACGC29150.034.0320784
ATCAACG29500.033.4674073
CAACGCA30200.033.0060165
AACGCAG30200.032.8313986
TATCGCT600.00397505431.6270567
GTACATG36400.029.9834291
ACGCAGA34050.029.2585097
TACATGG38050.028.564042
CGCAGAG35400.028.2767318
ACATGGG40050.026.3106273
CATGGGG32300.023.9533254
GCAGAGT40650.023.9256789
GAGTACT19550.021.838612-13
AGAGTAC35550.021.48400710-11
CAGAGTA38400.020.93955810-11
GTAATAC2151.0860585E-619.8694023
TATACCG1250.006055889718.9863195
Produced by FastQC (version 0.11.5)