FastQCFastQC Report
Fri 10 Feb 2017
SRR2031444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036840
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22150.21362987539060996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.17649782030014274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.062.3392751
GTATCAA25800.041.8339041
TCAACGC30750.034.5973744
ATCAACG31500.033.6228523
CAACGCA31650.033.6135645
AACGCAG31700.033.5605476
TATCAAC32250.032.9881942
GTACATG38050.028.6156121
ACGCAGA38000.027.8715747
TACATGG38750.027.6998212
CGCAGAG38850.027.2617748
TATAGGG700.00839622827.1395242
ACATGGG41100.026.0004543
CATGGGG32500.024.6969644
TCGCACT1201.712084E-423.7470844
GCAGAGT44650.023.0822729
GAGTACT22600.022.277112-13
CAGAGTA41450.021.9424210-11
CGTGTCG1100.002879229421.5882579
ATGGGGA13300.020.3546435
Produced by FastQC (version 0.11.5)