FastQCFastQC Report
Fri 10 Feb 2017
SRR2031443_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031443_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176307
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14160.1203767383854725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.050.4005781
GTATCAA19450.045.386431
TCAACGC24400.035.6014674
CAACGCA24950.034.816675
AACGCAG25400.034.1867376
ATCAACG25550.034.184843
TATCAAC26250.033.2732432
TACATGG34300.029.8927762
GTACATG34900.029.781831
ACGCAGA29200.029.5752777
CGCAGAG29750.028.869018
GTATACG856.438473E-427.9179671
ACATGGG36450.027.3481793
CATGGGG25350.024.7173924
GCAGAGT37050.022.6705639
TAACGCA1100.002888455521.5766494
AGAGTAC31150.021.2498810-11
TATACTG5200.020.9957395
GTATTGG5700.019.1507431
ATGGGGC12150.019.1437265
Produced by FastQC (version 0.11.5)