FastQCFastQC Report
Fri 10 Feb 2017
SRR2031443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176307
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16700.1419697408924711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14330.12182193934066532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.056.2452851
GTATCAA19800.043.95731
CAACGCA25200.034.4923675
TCAACGC25350.033.9135324
AACGCAG26050.033.3668946
ATCAACG26300.032.688523
TATCAAC26750.032.316182
GTACATG33800.027.2979551
ACGCAGA31450.027.1846837
TACATGG33850.026.8007622
CGCAGAG32750.025.8155358
ACATGGG35600.025.3498883
CATGGGG24050.022.9094874
GTATAAT6000.022.1948321
GCAGAGT38700.021.6010159
AGAGTAC34300.019.66376510-11
CAGAGTA36450.019.61152810-11
ATGGGGG11150.019.1694685
GTACCGT1507.7237363E-418.9990736
GAGTACT21050.018.05137612-13
Produced by FastQC (version 0.11.5)