FastQCFastQC Report
Fri 10 Feb 2017
SRR2031437_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031437_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490338
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24330.4961883435507752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18560.3785144125072909No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.20924341984508646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10550.069.268061
GTATCAA19650.042.985521
ATCAACG24650.034.0842823
TCAACGC24650.034.0842824
CAACGCA25050.033.7295155
TATCAAC24950.033.6744542
AACGCAG26200.032.4136356
GATCGTC300.003955936531.65804566-67
CGAGGAT404.4828415E-429.69915248-49
ACGCAGA28950.029.170747
CGCAGAG29100.028.857348
GTACATG24900.028.395641
ACTATAC2104.3655746E-1127.1243783
CTAGTCG908.975792E-426.3709244
TAGTCGA908.975792E-426.3709245
GCAGAGT32400.025.6253079
ACATGGG26100.025.2797133
TACATGG27450.024.9010682
ATGGGAC2900.024.5522385
CAGAGTA31600.023.7943510-11
Produced by FastQC (version 0.11.5)