##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031437_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 490338 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.96990239385893 34.0 33.0 34.0 31.0 34.0 2 33.044850694826835 34.0 33.0 34.0 31.0 34.0 3 33.20248685600545 34.0 34.0 34.0 31.0 34.0 4 36.16486994685299 37.0 37.0 37.0 35.0 37.0 5 36.153979499855204 37.0 37.0 37.0 35.0 37.0 6 36.23526832511451 37.0 37.0 37.0 35.0 37.0 7 36.27178599251945 37.0 37.0 37.0 35.0 37.0 8 36.35447181332061 37.0 37.0 37.0 35.0 37.0 9 38.316155386692444 39.0 39.0 39.0 38.0 39.0 10-11 38.406149431616555 39.0 39.0 39.0 37.5 39.0 12-13 38.46638849120403 39.0 39.0 39.0 38.0 39.0 14-15 40.096608869800015 41.0 40.0 41.0 39.0 41.0 16-17 40.09446035183893 41.0 40.0 41.0 39.0 41.0 18-19 40.148802458712154 41.0 40.5 41.0 39.0 41.0 20-21 40.100700945062385 41.0 40.5 41.0 39.0 41.0 22-23 40.03179133577247 41.0 40.0 41.0 39.0 41.0 24-25 39.932237558582045 41.0 40.0 41.0 38.0 41.0 26-27 39.84265955320616 41.0 40.0 41.0 38.0 41.0 28-29 39.78147522729219 41.0 40.0 41.0 38.0 41.0 30-31 39.61238574207995 41.0 40.0 41.0 37.5 41.0 32-33 39.41517075976164 41.0 40.0 41.0 36.5 41.0 34-35 39.338846877052156 41.0 40.0 41.0 36.0 41.0 36-37 39.22661103157414 41.0 40.0 41.0 36.0 41.0 38-39 39.05889406898915 41.0 39.5 41.0 35.0 41.0 40-41 38.960802548446175 41.0 39.0 41.0 35.0 41.0 42-43 39.099728146706966 41.0 40.0 41.0 35.5 41.0 44-45 39.046148779005506 41.0 40.0 41.0 35.5 41.0 46-47 38.8284091789745 41.0 40.0 41.0 35.0 41.0 48-49 38.606100281846395 41.0 40.0 41.0 35.0 41.0 50-51 37.61661955630606 40.0 38.0 40.5 34.0 40.5 52-53 37.68728917603775 39.5 38.5 40.5 34.5 41.0 54-55 38.12885193478784 40.5 39.0 41.0 35.0 41.0 56-57 38.15495127850585 41.0 39.0 41.0 35.0 41.0 58-59 38.194907390412325 41.0 39.0 41.0 35.0 41.0 60-61 38.018568823954084 41.0 38.5 41.0 35.0 41.0 62-63 37.79484253718863 41.0 37.5 41.0 34.5 41.0 64-65 37.55100155403008 40.0 37.0 41.0 34.0 41.0 66-67 37.29585510403028 39.5 36.5 41.0 34.0 41.0 68-69 36.9674204324364 39.0 36.0 41.0 34.0 41.0 70-71 36.59209259735121 39.0 35.5 41.0 33.5 41.0 72-73 36.20250317128185 37.0 35.0 40.0 33.0 41.0 74-75 35.77443824463941 37.0 35.0 39.0 33.0 41.0 76-77 35.3813859011539 36.5 35.0 39.0 32.5 41.0 78-79 34.963063233932516 36.0 35.0 38.0 32.0 39.5 80-81 34.54972488365169 36.0 35.0 37.0 32.0 39.0 82-83 34.21756523051447 35.0 35.0 37.0 31.5 39.0 84-85 33.89446157548467 35.0 35.0 36.0 31.0 37.5 86-87 33.65491354942917 35.0 35.0 36.0 31.0 37.0 88-89 33.46122470622305 35.0 35.0 36.0 31.0 37.0 90-91 33.251189995472515 35.0 35.0 35.5 30.0 36.0 92-93 33.18235380492639 35.0 35.0 35.0 30.0 36.0 94-95 33.08195571218221 35.0 35.0 35.0 30.0 36.0 96-97 32.99941366975433 35.0 35.0 35.0 30.0 36.0 98-99 32.93177359290938 35.0 35.0 35.0 30.0 36.0 100-101 32.614781028596596 35.0 34.0 35.0 28.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03238736671528386 1101 2 0.14176815245856034 1101 3 0.049301099715684416 1101 4 0.06292112926638538 1101 5 -0.013093067474663656 1101 6 0.1604562729236818 1101 7 0.16387950615852276 1101 8 0.07204589204565082 1101 9 0.14992411128189786 1101 10-11 0.0552489624641197 1101 12-13 -0.07605790879809149 1101 14-15 -0.018647897813032444 1101 16-17 -0.053701911569660865 1101 18-19 -0.020605402809074747 1101 20-21 -0.04604411823394372 1101 22-23 0.16803811042277061 1101 24-25 -0.05562902502374811 1101 26-27 -0.0757437560567027 1101 28-29 0.08896415098890031 1101 30-31 -5.939637257839081E-4 1101 32-33 -0.0796965697674139 1101 34-35 -0.1427928129379481 1101 36-37 0.03098107908252956 1101 38-39 0.06583723988826762 1101 40-41 0.016299617076612094 1101 42-43 -0.06629541402273986 1101 44-45 0.03510136167175659 1101 46-47 0.04500719625505667 1101 48-49 -0.11725388431680273 1101 50-51 -0.15824138344789418 1101 52-53 -0.02734264405842879 1101 54-55 -0.030718516996593337 1101 56-57 0.05904544973694925 1101 58-59 -0.062089146853828936 1101 60-61 0.05220069662028237 1101 62-63 0.16112632410970917 1101 64-65 -0.021990710862972662 1101 66-67 -0.0798673283509288 1101 68-69 -0.027871451264466884 1101 70-71 -0.02989026633325409 1101 72-73 -0.27088277536319083 1101 74-75 0.052739478228325254 1101 76-77 -0.04593428965433333 1101 78-79 -0.18179541704196112 1101 80-81 0.0749948951286541 1101 82-83 -0.14892802105055836 1101 84-85 -0.2617160203682616 1101 86-87 -0.0818701889223874 1101 88-89 -0.16218263233769648 1101 90-91 -0.21634140758552434 1101 92-93 -0.1485933548074172 1101 94-95 -0.16756068677477032 1101 96-97 -0.1734112550321143 1101 98-99 -0.18878998672873593 1101 100-101 0.06818041715158074 1102 1 -0.024165658295196124 1102 2 -0.05830403793778771 1102 3 -0.09343212183896554 1102 4 -0.18066324034844428 1102 5 -0.021173734420777635 1102 6 -0.12650637436801304 1102 7 -0.08881599009223606 1102 8 -0.10716676688966231 1102 9 -0.31308413239561617 1102 10-11 -0.25206380824987207 1102 12-13 -0.17360227096101255 1102 14-15 -0.13619598593189863 1102 16-17 -0.05284028425840148 1102 18-19 -9.771833502796312E-4 1102 20-21 -0.1252468800987785 1102 22-23 -0.2597452951009629 1102 24-25 -0.10854924299216862 1102 26-27 0.04668591652491472 1102 28-29 -0.023414173262416682 1102 30-31 0.0651866240437613 1102 32-33 0.25063853339498365 1102 34-35 0.057916865232606085 1102 36-37 0.10451340175031731 1102 38-39 0.19901105573289612 1102 40-41 0.03973529776219209 1102 42-43 0.20879948141762128 1102 44-45 0.3867646516908536 1102 46-47 0.21081805741662407 1102 48-49 0.12357386008261528 1102 50-51 0.07154239467143242 1102 52-53 0.136625615454399 1102 54-55 0.2157127158290706 1102 56-57 0.3490092381026457 1102 58-59 0.24125024115790694 1102 60-61 0.18886177435303608 1102 62-63 0.09572997554653284 1102 64-65 0.2662963973294623 1102 66-67 0.19001996148186606 1102 68-69 0.23876727073264448 1102 70-71 0.2615435280871239 1102 72-73 0.15520357366573023 1102 74-75 0.09287209897257753 1102 76-77 -0.0013159672659881494 1102 78-79 -0.030544893079408553 1102 80-81 0.05030170431314218 1102 82-83 0.05595880303774692 1102 84-85 0.40312259916505866 1102 86-87 0.13838038161461697 1102 88-89 0.13331769368344482 1102 90-91 0.11036671034097623 1102 92-93 0.17421124209072758 1102 94-95 0.2200900980047038 1102 96-97 0.11535615216829598 1102 98-99 0.10960086604942632 1102 100-101 0.05335809867549557 1103 1 0.18470792796730962 1103 2 0.1697408094112518 1103 3 0.12348260718106019 1103 4 0.3324390281690697 1103 5 0.13135737325208652 1103 6 0.2376059401300168 1103 7 0.16250785658126432 1103 8 0.04513007984920847 1103 9 0.11147295100127508 1103 10-11 -0.026544075852129367 1103 12-13 -0.007048194820754361 1103 14-15 -0.03843562941943901 1103 16-17 -0.176116014825638 1103 18-19 -0.1392755773009 1103 20-21 -0.0616831720205937 1103 22-23 -0.0970837640150819 1103 24-25 -0.08612012054452833 1103 26-27 -0.13869347722957315 1103 28-29 -0.05980361299742043 1103 30-31 -0.05521260017118834 1103 32-33 -0.023593853451536972 1103 34-35 0.036364859305265895 1103 36-37 0.01594914887925114 1103 38-39 0.05646055343386536 1103 40-41 -0.06904621850532067 1103 42-43 -0.013486905298151441 1103 44-45 0.18504964373688182 1103 46-47 0.23953408958119127 1103 48-49 0.1826539228427606 1103 50-51 0.1468382171997007 1103 52-53 0.21504582509070502 1103 54-55 0.19571609354486696 1103 56-57 0.3425834062039357 1103 58-59 0.36121090711271364 1103 60-61 0.4795932132599674 1103 62-63 0.34674873821297325 1103 64-65 0.35430223750902456 1103 66-67 0.3885397592547548 1103 68-69 0.304809786368196 1103 70-71 0.34897733575345313 1103 72-73 0.35256568659039544 1103 74-75 0.32357281156165385 1103 76-77 0.35966923615966806 1103 78-79 0.4231695047065571 1103 80-81 0.4647120205109232 1103 82-83 0.5593828262241303 1103 84-85 0.4950972554007649 1103 86-87 0.3671089440151931 1103 88-89 0.3175911579123536 1103 90-91 0.4693034925763726 1103 92-93 0.49620561982831646 1103 94-95 0.49989299194442083 1103 96-97 0.49851714935716274 1103 98-99 0.378240954087957 1103 100-101 0.41473555296737885 1104 1 0.2133999445325898 1104 2 0.2380180995216179 1104 3 0.11609163882550178 1104 4 0.09146472813142026 1104 5 0.3002301019658944 1104 6 0.28063901124579615 1104 7 0.2269856341388703 1104 8 0.12593040481403506 1104 9 0.2512771795070279 1104 10-11 0.08684437600750528 1104 12-13 0.13250834707790204 1104 14-15 0.19262872946854515 1104 16-17 0.21477308101917458 1104 18-19 0.14063258050519067 1104 20-21 0.12982563571515726 1104 22-23 0.15078860803001248 1104 24-25 0.014088926951863812 1104 26-27 0.22095986059513706 1104 28-29 0.144352072896325 1104 30-31 0.31360266524977476 1104 32-33 0.2470037647940515 1104 34-35 0.21016159776802112 1104 36-37 0.11980662009806764 1104 38-39 0.23416083282435807 1104 40-41 0.19891353737475015 1104 42-43 0.15098690927374747 1104 44-45 0.05317138665799348 1104 46-47 0.3042446925494744 1104 48-49 0.24889770590891658 1104 50-51 0.3774195872038604 1104 52-53 0.365859723281595 1104 54-55 0.47810607115399506 1104 56-57 0.5115477789623739 1104 58-59 0.37267136642275744 1104 60-61 0.3919393998769678 1104 62-63 0.4016559052726265 1104 64-65 0.4007187076776546 1104 66-67 0.5706959207067825 1104 68-69 0.19799989079287172 1104 70-71 0.10180096307427533 1104 72-73 0.15593719668610362 1104 74-75 0.3540803709569005 1104 76-77 0.11615862526542031 1104 78-79 0.36209989486249583 1104 80-81 0.10518747972189146 1104 82-83 0.21552694019960228 1104 84-85 0.17803115279906478 1104 86-87 -0.013602709144294067 1104 88-89 -0.09160445506245907 1104 90-91 -0.10088593814072766 1104 92-93 0.042225791425593684 1104 94-95 0.22869349524361127 1104 96-97 0.2656000946481427 1104 98-99 0.1485983163246729 1104 100-101 -0.04541167195187512 1105 1 -0.03480330265590936 1105 2 -0.023334957751039553 1105 3 0.033538760297162185 1105 4 -0.06419178486478216 1105 5 0.03639138147732979 1105 6 0.04035823370799818 1105 7 -0.01757755942632855 1105 8 0.07965238291785681 1105 9 0.16817968937520789 1105 10-11 0.14937928700773284 1105 12-13 0.05255517436620494 1105 14-15 0.04222515830596052 1105 16-17 0.05999399782858461 1105 18-19 0.11090237271492498 1105 20-21 0.08512559102907602 1105 22-23 0.05125734639301527 1105 24-25 0.11767219201478696 1105 26-27 0.024704181739103603 1105 28-29 0.124038525566327 1105 30-31 0.10473480571302218 1105 32-33 0.15073784537795376 1105 34-35 0.2111414331742978 1105 36-37 0.18484348611431045 1105 38-39 0.214163811896384 1105 40-41 0.07034796055193482 1105 42-43 0.17734471444244093 1105 44-45 0.11215934003551808 1105 46-47 0.05390256474728261 1105 48-49 0.09830561534513294 1105 50-51 0.1381582108725894 1105 52-53 0.07257706520459095 1105 54-55 0.07908963040028993 1105 56-57 0.06603919551111659 1105 58-59 0.009285092497087533 1105 60-61 0.05260215030520499 1105 62-63 0.05845982647617376 1105 64-65 0.025697761077516645 1105 66-67 0.07412185352870893 1105 68-69 0.144753633252094 1105 70-71 0.31358505482969434 1105 72-73 0.1302962307220028 1105 74-75 -0.04065132636937818 1105 76-77 0.22709647437542202 1105 78-79 0.0380955565888641 1105 80-81 0.16407535489877034 1105 82-83 0.22746650227601606 1105 84-85 0.11100203237007378 1105 86-87 0.005435422977548399 1105 88-89 0.02286638727014889 1105 90-91 0.043103318580797634 1105 92-93 -0.15448838523337827 1105 94-95 -0.23756153194042184 1105 96-97 -0.3341406329901915 1105 98-99 -0.35915425312494875 1105 100-101 -0.1372159655394256 1106 1 -0.10373319721276886 1106 2 0.034249111784440345 1106 3 0.04502707605663403 1106 4 -0.20753746739541867 1106 5 -0.12300232043204318 1106 6 -0.1001376340605944 1106 7 -0.11603865945482994 1106 8 0.02069684002814398 1106 9 0.051479774869648054 1106 10-11 0.03486126192935757 1106 12-13 -0.14861503082045147 1106 14-15 -0.2082646536062569 1106 16-17 -0.023176064867342916 1106 18-19 0.009039306314242879 1106 20-21 -0.0800033630889132 1106 22-23 -0.0709729898851208 1106 24-25 -0.007986827648927886 1106 26-27 -0.04931975670489663 1106 28-29 0.007089252269082635 1106 30-31 0.0962298897825633 1106 32-33 -0.05984565419458221 1106 34-35 0.09455729239344635 1106 36-37 0.38988765824311855 1106 38-39 0.17041915529905793 1106 40-41 0.052750354396337684 1106 42-43 0.16613247448803747 1106 44-45 0.11779662986167949 1106 46-47 -0.15408938452601006 1106 48-49 0.09007677521974244 1106 50-51 -0.02209043546539391 1106 52-53 -3.5147569025184566E-4 1106 54-55 -0.008497645176795743 1106 56-57 -0.1031952729984269 1106 58-59 0.11887828143980528 1106 60-61 -0.04194936009661632 1106 62-63 -0.00868002818327085 1106 64-65 0.24533832819066248 1106 66-67 0.3927667666093697 1106 68-69 0.2479347389802271 1106 70-71 0.14647904172054638 1106 72-73 0.21100832818567028 1106 74-75 -0.055559419843298485 1106 76-77 0.03863466189323361 1106 78-79 0.15011999375045804 1106 80-81 0.05829379320028494 1106 82-83 0.14701266927516343 1106 84-85 0.12300687705516822 1106 86-87 -0.038380478988820244 1106 88-89 0.13960192446023 1106 90-91 0.24008785833571977 1106 92-93 0.0994924497623515 1106 94-95 0.11673278267912224 1106 96-97 -0.0492214964840727 1106 98-99 -0.027106304991576735 1106 100-101 0.11152833483104985 1107 1 0.21840799010408318 1107 2 0.24310789154144175 1107 3 0.17211377060077382 1107 4 0.2772012636308858 1107 5 0.17786857614692764 1107 6 0.08717098271198154 1107 7 0.22575943741389182 1107 8 0.1509362131143135 1107 9 0.12830470654789394 1107 10-11 0.02535383708328709 1107 12-13 0.010331826378845221 1107 14-15 0.1284792797512182 1107 16-17 0.12536612998959384 1107 18-19 -0.015737619042191398 1107 20-21 0.05533956272848428 1107 22-23 -0.044664998709329495 1107 24-25 0.02194303965870148 1107 26-27 -0.08669504892444735 1107 28-29 -0.10583508344590342 1107 30-31 0.01544970473761964 1107 32-33 0.26188807205743814 1107 34-35 -0.020453737865686605 1107 36-37 -0.04571703044016573 1107 38-39 -0.029516638714966348 1107 40-41 0.05743409875773864 1107 42-43 -0.06421061596009281 1107 44-45 -0.09335051708770692 1107 46-47 0.09569659552074228 1107 48-49 0.017593979131369508 1107 50-51 -0.022329150028632228 1107 52-53 -0.01609840532707807 1107 54-55 0.19702736223158723 1107 56-57 0.14715868307809643 1107 58-59 0.2096474362256231 1107 60-61 0.257184682246411 1107 62-63 0.2772012401318946 1107 64-65 0.3909994852154526 1107 66-67 0.44817735565813166 1107 68-69 0.18994356318752637 1107 70-71 0.04691037424169764 1107 72-73 0.20175608097615338 1107 74-75 0.3479752019999012 1107 76-77 0.3104441182825539 1107 78-79 0.31193554147689895 1107 80-81 0.26581494824950624 1107 82-83 0.16883035720113782 1107 84-85 0.37233600681711465 1107 86-87 0.19260055555070466 1107 88-89 0.10453969404306207 1107 90-91 0.025866232442588455 1107 92-93 0.30868049098588557 1107 94-95 0.4401457424104507 1107 96-97 0.3902849405775939 1107 98-99 0.2725216295512425 1107 100-101 0.5287108444619477 1108 1 0.13954607925690254 1108 2 0.08340707217739407 1108 3 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-0.7281928070118937 2316 54-55 -0.697538787339063 2316 56-57 -0.5729635527741337 2316 58-59 -0.5035461111109143 2316 60-61 -0.4733509409284693 2316 62-63 -0.5300167543750902 2316 64-65 -0.5994637300434036 2316 66-67 -0.4943034025081161 2316 68-69 -0.7398712375550147 2316 70-71 -1.351770108188255 2316 72-73 -1.0262121550714767 2316 74-75 -0.7074217388470672 2316 76-77 -0.6144014585382322 2316 78-79 -0.5746869060626381 2316 80-81 -0.6171599640902343 2316 82-83 -0.6825271555272323 2316 84-85 -0.6142164210735075 2316 86-87 -0.5286832465705089 2316 88-89 -0.5609210786699066 2316 90-91 -0.6284266776608618 2316 92-93 -0.6725254753395475 2316 94-95 -0.7423857121527675 2316 96-97 -0.775677911013311 2316 98-99 -0.9172587584335972 2316 100-101 -0.821759209925407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 170.0 3 53.0 4 51.0 5 68.0 6 78.0 7 200.0 8 456.0 9 332.0 10 231.0 11 255.0 12 244.0 13 308.0 14 302.0 15 334.0 16 476.0 17 625.0 18 968.0 19 1339.0 20 1916.0 21 2928.0 22 4053.0 23 3845.0 24 2875.0 25 1950.0 26 1701.0 27 1530.0 28 1712.0 29 1878.0 30 2262.0 31 2757.0 32 3522.0 33 4944.0 34 7644.0 35 13597.0 36 28307.0 37 85235.0 38 217557.0 39 93545.0 40 90.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.58915035533637 20.918630725628905 14.345877057492466 22.14634186154226 2 14.92289670035429 22.314037780208658 41.03107805841728 21.731987461019774 3 16.78837925356415 29.14776703823183 32.060299251768555 22.003554456435463 4 11.61559166664966 19.14021765969908 41.83714907832361 27.40704159532764 5 11.855618542916575 39.30234409607466 36.212742590366 12.629294770642762 6 26.720260253396027 39.763951475966564 20.460464015151516 13.055324255485893 7 24.46767032406226 34.729063536616486 24.733860678744577 16.06940546057668 8 24.330554013001013 34.50127396857544 23.05209388168425 18.116078136739294 9 26.726677677986277 15.432248738768353 22.324055377818247 35.51701820542713 10-11 22.426338167425637 27.35127590038498 31.316367584202077 18.906018347987303 12-13 24.202583766324526 26.215138385447005 29.813496674648054 19.768781173580415 14-15 21.94963867227371 27.103764340832075 28.35718368513453 22.589413301759688 16-17 20.134202168414845 29.75739749409027 29.097405227497873 21.010995109997012 18-19 19.836229776070535 28.693550986436122 31.62164753498069 19.84857170251265 20-21 21.64616668332779 27.755562945387556 31.37038087330133 19.227889497983323 22-23 21.27318242539221 28.049140159466717 30.559419363990486 20.118258051150587 24-25 20.881547764975075 28.125459671488112 30.98369698455504 20.009295578981774 26-27 20.614666387443112 28.214586706575208 31.053166548337174 20.11758035764451 28-29 20.63208109719738 27.829273572344615 31.3755401442563 20.16310518620171 30-31 21.185364020359977 27.685727591362536 31.10360139545904 20.02530699281845 32-33 20.556119831847937 28.051026017792076 31.217978455772204 20.174875694587787 34-35 21.119857479370673 28.219023316914317 30.89823136311911 19.762887840595905 36-37 21.328288441422593 27.64795534780335 30.40296482740586 20.6207913833682 38-39 20.634211792934376 28.39421645279996 30.85055831525421 20.121013439011453 40-41 20.897325650510542 27.847611305269655 31.203569612134558 20.05149343208525 42-43 20.787173350615156 27.860711242198942 30.56851408655539 20.783601320630517 44-45 21.011578654133245 28.17020121572297 30.306440964374236 20.51177916576955 46-47 21.21751465763141 27.997632326902178 29.860950232940255 20.923902782526163 48-49 20.962882419304613 28.261329004395304 29.854153585412295 20.92163499088779 50-51 21.245102574077876 28.21519777074584 29.44183895088636 21.097860704289925 52-53 21.05150810737937 28.480617051438657 29.40390641277571 21.063968428406262 54-55 21.6028341103083 28.450673418052734 29.12557353222257 20.820918939416394 56-57 21.36500729491481 28.7437012395225 28.94712106289157 20.944170402671126 58-59 20.982730028020814 28.5081162332835 29.397756700896178 21.11139703779951 60-61 21.737397195583423 28.57875896695744 29.014201984230013 20.669641853229127 62-63 21.349759475322966 28.56785085630056 29.240359079934336 20.84203058844214 64-65 21.272982503494088 28.315855230803994 29.294915247305692 21.11624701839623 66-67 21.302877845501268 28.61201112212795 28.80040926816766 21.284701764203128 68-69 21.352930184685416 28.499164233908314 28.967131979565853 21.18077360184042 70-71 21.35993513615077 28.273980324360092 29.200475450380992 21.16560908910814 72-73 21.46814121511344 28.201862523005776 28.892597502609537 21.43739875927125 74-75 21.492245845113004 28.3478093899629 28.61683525893485 21.543109505989246 76-77 21.15275617569835 28.399480801183824 29.045313474965813 21.402449548152013 78-79 21.0193379789161 28.51016140352311 28.899188947040972 21.571311670519826 80-81 21.225256066922505 28.699843655256263 28.611803874772875 21.463096403048365 82-83 21.49268720718915 28.638166836604768 28.617815172114742 21.251330784091337 84-85 21.342140847316095 28.48405622608996 28.632971759196323 21.540831167397616 86-87 20.990820361809188 28.79693691796791 28.854788774286792 21.35745394593611 88-89 21.371757394877246 28.43772358388356 28.81712626995646 21.373392751282733 90-91 21.45237414187643 28.528624550506702 28.888729977116707 21.130271330500165 92-93 21.493452306171204 28.655519091524717 28.786258473801563 21.064770128502513 94-95 21.268173518033603 28.46701090308955 28.87051508825824 21.394300490618605 96-97 21.504111488580445 28.365237831208916 28.83096487492967 21.299685805280962 98-99 21.175814591881963 28.59267035810793 28.619324715431542 21.612190334578564 100-101 21.158458847854337 28.486245842288927 28.9684177406569 21.38687756919984 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 285.0 1 195.0 2 100.5 3 98.0 4 86.0 5 98.5 6 130.0 7 188.5 8 333.0 9 420.0 10 348.0 11 292.5 12 337.0 13 390.0 14 427.0 15 475.5 16 504.5 17 516.5 18 558.5 19 629.0 20 713.5 21 785.5 22 897.5 23 1081.5 24 1309.5 25 1666.0 26 2337.0 27 3320.0 28 4200.5 29 5506.0 30 7255.5 31 8886.0 32 11238.5 33 13971.5 34 16411.5 35 18668.5 36 21160.5 37 23321.5 38 22783.5 39 21546.0 40 20778.5 41 19407.0 42 18651.0 43 18811.0 44 18461.0 45 18279.0 46 17820.0 47 16176.0 48 15975.0 49 16011.5 50 15050.5 51 13931.0 52 12759.0 53 11733.0 54 10664.5 55 9422.0 56 8594.0 57 7554.0 58 6153.5 59 5023.0 60 4066.0 61 3291.5 62 2450.5 63 1629.5 64 1049.0 65 771.5 66 629.5 67 502.5 68 384.0 69 246.5 70 140.0 71 104.0 72 87.0 73 70.0 74 66.5 75 61.5 76 48.0 77 30.5 78 17.0 79 18.5 80 17.5 81 15.0 82 11.5 83 7.5 84 5.5 85 5.0 86 5.5 87 4.0 88 4.0 89 4.5 90 3.0 91 1.5 92 1.5 93 1.5 94 1.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04874188824851429 2 0.07056357043508764 3 0.05078129779866133 4 0.06260987318951416 5 0.09564830790189624 6 0.07219509807520527 7 0.09442466217180802 8 0.1080887061577932 9 0.10992417475292553 10-11 0.06352760748708033 12-13 0.048843858726021636 14-15 0.09748377649702858 16-17 0.052310854961271615 18-19 0.028347792747043875 20-21 0.035689667127573224 22-23 0.08004682484327137 24-25 0.17498133940261615 26-27 0.1805897156655205 28-29 0.1333773845796165 30-31 0.1529557162610281 32-33 0.20781583315998353 34-35 0.17640892608771908 36-37 0.1766128670427338 38-39 0.21485179610799082 40-41 0.15519906676618986 42-43 0.0857571715836831 44-45 0.06964583613752147 46-47 0.08269805725846252 48-49 0.12450595303647688 50-51 0.13613058747231502 52-53 0.15988970873152805 54-55 0.23544983256447594 56-57 0.19496755299405716 58-59 0.1431665504203223 60-61 0.24085426787236558 62-63 0.24452520506263029 64-65 0.2646133891315786 66-67 0.13939364275255028 68-69 0.13613058747231502 70-71 0.1421468456452488 72-73 0.16050153159657216 74-75 0.16192911828167508 76-77 0.15040645432334432 78-79 0.18609612145091753 80-81 0.2762380235674168 82-83 0.2925532999685931 84-85 0.23137101346418187 86-87 0.23606165542952004 88-89 0.23412421635688033 90-91 0.18313897760320433 92-93 0.16610990785947655 94-95 0.15336359817105752 96-97 0.13735423320240323 98-99 0.15030448384583697 100-101 0.18069168614302786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 490338.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.83107097613467 #Duplication Level Percentage of deduplicated Percentage of total 1 64.35333981270347 26.91969125256512 2 15.803633459230406 13.221658258277674 3 7.153058593630293 8.976603051797964 4 3.7984422369474906 6.3557162724999285 5 2.2821711964507787 4.773283264921135 6 1.532103139873256 3.8453709112078176 7 1.0349522631443475 3.030521310355168 8 0.7285989719430197 2.4382460246789766 9 0.5270854947516652 1.984369566730317 >10 2.5517854347436533 18.541398040409675 >50 0.173655352094734 4.931187634356507 >100 0.059694027281604625 3.8851030928326007 >500 0.0 0.0 >1k 0.0014800172053290404 1.0968513193671132 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2433 0.4961883435507752 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1856 0.3785144125072909 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1026 0.20924341984508646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.0394095501470415E-4 0.0 4 0.0 0.0 0.0 2.0394095501470415E-4 0.0 5 0.0 0.0 0.0 2.0394095501470415E-4 0.0 6 0.0 0.0 0.0 2.0394095501470415E-4 0.0 7 0.0 0.0 0.0 2.0394095501470415E-4 0.0 8 0.0 0.0 0.0 2.0394095501470415E-4 0.0 9 0.0 0.0 0.0 2.0394095501470415E-4 0.0 10-11 0.0 0.0 0.0 2.0394095501470415E-4 0.0 12-13 0.0 0.0 0.0 2.0394095501470415E-4 0.0 14-15 0.0 0.0 0.0 2.0394095501470415E-4 0.0 16-17 0.0 0.0 0.0 6.118228650441124E-4 0.0 18-19 0.0 0.0 0.0 8.157638200588166E-4 0.0 20-21 0.0 0.0 0.0 8.157638200588166E-4 0.0 22-23 0.0 0.0 0.0 0.0017334981176249853 0.0 24-25 0.0 0.0 0.0 0.0032630552802352664 0.0 26-27 0.0 0.0 0.0 0.0037729076677720267 0.0 28-29 0.0 0.0 0.0 0.006424140082963181 0.0 30-31 0.0 0.0 0.0 0.010400988705749912 0.0 32-33 0.0 0.0 0.0 0.01713104022123515 0.0 34-35 0.0 0.0 0.0 0.02793991083701447 0.0 36-37 0.0 0.0 0.0 0.04517292153575697 0.0 38-39 0.0 0.0 0.0 0.07035962948007293 0.0 40-41 0.0 0.0 0.0 0.0957502783794036 0.0 42-43 0.0 0.0 0.0 0.11033205666295495 0.0 44-45 0.0 0.0 0.0 0.12797294927172684 0.0 46-47 0.0 0.0 0.0 0.15458724390114573 0.0 48-49 0.0 0.0 0.0 0.18130350900807196 0.0 50-51 0.0 0.0 0.0 0.20669415790740264 0.0 52-53 0.0 0.0 0.0 0.23137101346418185 0.0 54-55 0.0 0.0 0.0 0.2549261937683802 0.0 56-57 0.0 0.0 0.0 0.28378383890296077 0.0 58-59 0.0 0.0 0.0 0.31457892311018115 0.0 60-61 0.0 0.0 0.0 0.34343656824476176 0.0 62-63 0.0 0.0 0.0 0.37443559340699684 0.0 64-65 0.0 0.0 0.0 0.40370112045160683 0.0 66-67 0.0 0.0 0.0 0.43775925993906245 0.0 68-69 0.0 0.0 0.0 0.4764060709143489 0.0 70-71 0.0 0.0 0.0 0.5227006677026867 0.0 72-73 0.0 0.0 0.0 0.5669558549408775 0.0 74-75 0.0 0.0 0.0 0.614881979369333 0.0 76-77 0.0 0.0 0.0 0.6590351961300165 0.0 78-79 0.0 0.0 0.0 0.7131815196864204 0.0 80-81 0.0 0.0 0.0 0.7662061679902434 0.0 82-83 0.0 0.0 0.0 0.825145103989493 0.0 84-85 0.0 0.0 0.0 0.888570740999066 0.0 86-87 0.0 0.0 0.0 0.946795883655764 0.0 88-89 0.0 0.0 0.0 1.0135865464230795 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1055 0.0 69.26806 1 GTATCAA 1965 0.0 42.98552 1 ATCAACG 2465 0.0 34.084282 3 TCAACGC 2465 0.0 34.084282 4 CAACGCA 2505 0.0 33.729515 5 TATCAAC 2495 0.0 33.674454 2 AACGCAG 2620 0.0 32.413635 6 GATCGTC 30 0.0039559365 31.658045 66-67 CGAGGAT 40 4.4828415E-4 29.699152 48-49 ACGCAGA 2895 0.0 29.17074 7 CGCAGAG 2910 0.0 28.85734 8 GTACATG 2490 0.0 28.39564 1 ACTATAC 210 4.3655746E-11 27.124378 3 CTAGTCG 90 8.975792E-4 26.370924 4 TAGTCGA 90 8.975792E-4 26.370924 5 GCAGAGT 3240 0.0 25.625307 9 ACATGGG 2610 0.0 25.279713 3 TACATGG 2745 0.0 24.901068 2 ATGGGAC 290 0.0 24.552238 5 CAGAGTA 3160 0.0 23.79435 10-11 >>END_MODULE