FastQCFastQC Report
Fri 10 Feb 2017
SRR2031437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490338
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25990.5300425420832161No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22020.4490779829423785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.24187397264743912No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT9100.18558626906338077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.065.0728761
ACCGCGA205.3908146E-447.49459524-25
GTATCAA20400.046.173031
TCAACGC24500.037.9956784
CGCGAGG250.00161660437.99567426-27
ATCAACG24800.037.7275623
CAACGCA24850.037.4605265
TATCAAC26150.035.961492
AACGCAG25950.035.87266
TCCGTTA300.003950899531.66629418-19
TCCCGGT300.003950899531.66629416-17
CCGGTGA300.003950899531.66629418-19
ACGCAGA29400.031.6630617
CGCAGAG29600.031.288678
GCAGAGT33750.027.1598729
CGGTGAG350.00839146227.14253636-37
GACCGCG350.00839563827.13976924-25
TACGAGA350.00839563827.13976922-23
CCGCGAG350.00839563827.13976926-27
GTACATG26400.027.0296691
Produced by FastQC (version 0.11.5)