##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031437_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 490338 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.33929044862931 34.0 33.0 34.0 31.0 34.0 2 33.43654581125673 34.0 34.0 34.0 31.0 34.0 3 33.5166211878337 34.0 34.0 34.0 31.0 34.0 4 36.67288686579462 37.0 37.0 37.0 35.0 37.0 5 36.6040914634395 37.0 37.0 37.0 35.0 37.0 6 36.64899722232419 37.0 37.0 37.0 37.0 37.0 7 36.65611680106375 37.0 37.0 37.0 37.0 37.0 8 36.668738706769616 37.0 37.0 37.0 37.0 37.0 9 38.612400017946804 39.0 39.0 39.0 38.0 39.0 10-11 38.62346381475635 39.0 39.0 39.0 38.5 39.0 12-13 38.62774351569733 39.0 39.0 39.0 39.0 39.0 14-15 40.325401049888036 41.0 41.0 41.0 39.0 41.0 16-17 40.36838670468126 41.0 41.0 41.0 39.0 41.0 18-19 40.42892657717738 41.0 41.0 41.0 39.5 41.0 20-21 40.43487451513038 41.0 41.0 41.0 39.0 41.0 22-23 40.38750412980434 41.0 41.0 41.0 39.0 41.0 24-25 40.35598403550204 41.0 41.0 41.0 39.0 41.0 26-27 40.27818973850691 41.0 41.0 41.0 39.0 41.0 28-29 40.20867442458059 41.0 40.0 41.0 39.0 41.0 30-31 40.0545593039903 41.0 40.0 41.0 38.0 41.0 32-33 39.89687011816339 41.0 40.0 41.0 38.0 41.0 34-35 39.82158735402926 41.0 40.0 41.0 37.0 41.0 36-37 39.73063172750226 41.0 40.0 41.0 37.0 41.0 38-39 39.62559907655536 41.0 40.0 41.0 37.0 41.0 40-41 39.53374712953106 41.0 40.0 41.0 37.0 41.0 42-43 39.339178281104054 41.0 40.0 41.0 36.0 41.0 44-45 39.18280655384653 41.0 40.0 41.0 36.0 41.0 46-47 38.96182531233557 41.0 40.0 41.0 35.5 41.0 48-49 38.70811256724953 41.0 39.5 41.0 35.0 41.0 50-51 38.59040498594847 41.0 39.0 41.0 35.0 41.0 52-53 38.61507266416227 41.0 39.0 41.0 35.0 41.0 54-55 38.56484914487557 41.0 39.0 41.0 35.0 41.0 56-57 38.491233598048694 41.0 39.0 41.0 35.0 41.0 58-59 38.464816106440864 41.0 39.0 41.0 35.0 41.0 60-61 38.30576153592013 41.0 39.0 41.0 35.0 41.0 62-63 38.109127785323594 41.0 38.0 41.0 35.0 41.0 64-65 37.860828652888415 40.0 37.0 41.0 35.0 41.0 66-67 37.566070751196115 39.5 37.0 41.0 35.0 41.0 68-69 37.198013411157206 39.0 36.0 41.0 34.0 41.0 70-71 36.82318421170703 39.0 36.0 41.0 34.0 41.0 72-73 36.43974462513613 37.0 35.0 40.0 34.0 41.0 74-75 36.01434622648051 37.0 35.0 39.0 34.0 41.0 76-77 34.84074964616244 36.0 34.5 39.0 31.5 39.5 78-79 35.152431588006635 36.0 35.0 38.0 33.0 39.5 80-81 34.883871941395526 36.0 35.0 37.0 33.0 39.0 82-83 34.56719548556302 35.0 35.0 37.0 33.0 39.0 84-85 34.263800684425846 35.0 35.0 36.5 33.0 38.0 86-87 34.046948227549166 35.0 35.0 36.0 33.0 37.0 88-89 33.84440018925721 35.0 35.0 36.0 33.0 37.0 90-91 33.694120178325974 35.0 35.0 35.5 33.0 36.0 92-93 33.585801018889015 35.0 35.0 35.0 32.0 36.0 94-95 33.4909633762833 35.0 35.0 35.0 32.0 36.0 96-97 33.41851641112865 35.0 35.0 35.0 32.0 36.0 98-99 33.36726706883823 35.0 35.0 35.0 32.0 36.0 100-101 33.14587488630292 35.0 34.5 35.0 31.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04954684240138363 1101 2 0.05950442775912279 1101 3 0.085931605085662 1101 4 0.11384476245874708 1101 5 0.12235335843364226 1101 6 0.1551909692768163 1101 7 0.11195399978943499 1101 8 0.09013272107851833 1101 9 0.1273425382865554 1101 10-11 0.026581698170097923 1101 12-13 0.06265685316758862 1101 14-15 0.06030768077476267 1101 16-17 0.05558717076550579 1101 18-19 0.08928005965758956 1101 20-21 0.11836542646624082 1101 22-23 0.17775788634125433 1101 24-25 0.14600273459322466 1101 26-27 0.1756728144738986 1101 28-29 0.07750771017796154 1101 30-31 0.17579536962461617 1101 32-33 0.2831635198133995 1101 34-35 0.3230547048971957 1101 36-37 0.3058795772221643 1101 38-39 0.32649581017393103 1101 40-41 0.2766945381557022 1101 42-43 0.2670009174989758 1101 44-45 0.2672970165076123 1101 46-47 0.15094676642565474 1101 48-49 0.10610886587098634 1101 50-51 0.23070728218078784 1101 52-53 0.20544769712417832 1101 54-55 0.13457735885549482 1101 56-57 0.0245036687356901 1101 58-59 0.1000966035650066 1101 60-61 0.3320312492595292 1101 62-63 0.12148082782714198 1101 64-65 2.578495845426687E-4 1101 66-67 -0.13820971828830153 1101 68-69 0.059758354042720896 1101 70-71 0.0559794203232471 1101 72-73 0.11733599034042896 1101 74-75 -0.13345312084964434 1101 76-77 -0.01262389904103145 1101 78-79 0.1403878105974954 1101 80-81 0.06638347876981499 1101 82-83 -0.0718216615015379 1101 84-85 -0.166343702287314 1101 86-87 -0.14963773065425556 1101 88-89 0.04459727646899836 1101 90-91 -0.09233111394109272 1101 92-93 -0.05869870977365821 1101 94-95 -0.11743818831023134 1101 96-97 0.12512398247790912 1101 98-99 -0.03344347517839452 1101 100-101 -0.02755813325640588 1102 1 0.01583276294537228 1102 2 0.04873990817456786 1102 3 0.10991744645308898 1102 4 0.04419267359469359 1102 5 0.0738984657877424 1102 6 0.08746007644462139 1102 7 -0.07103118953570231 1102 8 0.03119275554360712 1102 9 0.030851923038078155 1102 10-11 0.07848018915794341 1102 12-13 0.20632156370739096 1102 14-15 0.24664914134948646 1102 16-17 0.18608586668092642 1102 18-19 0.17878450751749142 1102 20-21 0.11183627086101211 1102 22-23 0.1089033849207297 1102 24-25 0.1004558341228261 1102 26-27 0.07206795210139916 1102 28-29 0.08984120652186078 1102 30-31 0.1244732374463311 1102 32-33 0.17534949158928015 1102 34-35 0.27824717380875796 1102 36-37 0.1644882819867277 1102 38-39 0.0947762815073574 1102 40-41 0.03396306314289177 1102 42-43 0.22198962319502868 1102 44-45 0.12005189518117021 1102 46-47 0.027247940101773338 1102 48-49 0.16258911830449563 1102 50-51 0.1218016653022147 1102 52-53 0.17718865003740802 1102 54-55 0.10955814689517496 1102 56-57 6.37174394491069E-4 1102 58-59 -0.0930447266012635 1102 60-61 -0.06000044465319121 1102 62-63 0.004369375170071521 1102 64-65 -0.034280015442710976 1102 66-67 -0.13092372788263873 1102 68-69 -0.07068212381111749 1102 70-71 0.2576322310912573 1102 72-73 0.1720580573144943 1102 74-75 0.10375346012003916 1102 76-77 -0.03529983402309966 1102 78-79 0.04176350339243129 1102 80-81 0.20768453600981474 1102 82-83 0.15340073149366873 1102 84-85 0.26940102315631265 1102 86-87 0.12289557007696317 1102 88-89 0.1285972066073171 1102 90-91 0.10863763460346121 1102 92-93 0.04328769048716907 1102 94-95 -0.36011726706762204 1102 96-97 0.011540544352058646 1102 98-99 0.09188254596640633 1102 100-101 0.06699090963866183 1103 1 0.021596671507339238 1103 2 0.0020075758073332395 1103 3 0.09735452287662838 1103 4 0.10642436310634196 1103 5 0.05986460146296224 1103 6 0.03673441234211339 1103 7 0.11283095607653593 1103 8 0.1517445217620903 1103 9 -0.01881684289710961 1103 10-11 0.06641575105730624 1103 12-13 0.03990096182022995 1103 14-15 0.035359398709637446 1103 16-17 0.01435898224239196 1103 18-19 -0.05793616628233167 1103 20-21 -0.0814344476118336 1103 22-23 -0.05074544159542427 1103 24-25 -0.06701894196730507 1103 26-27 0.01013045073941754 1103 28-29 -0.09955875861138708 1103 30-31 -0.06776237457398082 1103 32-33 -0.06579762427726621 1103 34-35 0.012241159295477644 1103 36-37 -0.00979095704659727 1103 38-39 -0.050887712042296585 1103 40-41 2.6217622010449304E-4 1103 42-43 0.3607565390136358 1103 44-45 0.36041178535092655 1103 46-47 0.2796232370138867 1103 48-49 0.3937392850111152 1103 50-51 0.4466071742477169 1103 52-53 0.45666194429092855 1103 54-55 0.5406418488716795 1103 56-57 0.3465152715419535 1103 58-59 0.4733764200787647 1103 60-61 0.5032963149188205 1103 62-63 0.3209321679962329 1103 64-65 0.4583325932434761 1103 66-67 0.45192564778472644 1103 68-69 0.38824594173835436 1103 70-71 0.47589532246391997 1103 72-73 0.4155123709844375 1103 74-75 0.27025033300895984 1103 76-77 0.24376214161376453 1103 78-79 0.6172608992825133 1103 80-81 0.6079602012019123 1103 82-83 0.5237890598512323 1103 84-85 0.41332440348710975 1103 86-87 0.47476638879168576 1103 88-89 0.5538053174835724 1103 90-91 0.5523441873720998 1103 92-93 0.5602525404512093 1103 94-95 0.6897775879085088 1103 96-97 0.5593297748866135 1103 98-99 0.5006678757840106 1103 100-101 0.6121814781844961 1104 1 0.12951867994849664 1104 2 0.07973163311211096 1104 3 0.07420077884587784 1104 4 0.013031651037067604 1104 5 0.05806144296334992 1104 6 0.09582748306159061 1104 7 0.07639625617363066 1104 8 0.056613049711046415 1104 9 0.10420909484437857 1104 10-11 0.09224203357855743 1104 12-13 0.13360823588050863 1104 14-15 0.14374953483820008 1104 16-17 0.008020681966961263 1104 18-19 0.002135266535155722 1104 20-21 0.04456868314688478 1104 22-23 0.0758595676185081 1104 24-25 0.10552450226357735 1104 26-27 0.01825952518410645 1104 28-29 0.0610734366845449 1104 30-31 0.21112283754483485 1104 32-33 0.08530111351178249 1104 34-35 0.2423073411568879 1104 36-37 0.17256583055718266 1104 38-39 0.34893048598809884 1104 40-41 0.30428262522638505 1104 42-43 0.4351789338459042 1104 44-45 0.3659079511622494 1104 46-47 0.27476362578278923 1104 48-49 0.15932372632920533 1104 50-51 0.3205545061675821 1104 52-53 0.4428063554903261 1104 54-55 0.4969598388453349 1104 56-57 0.5078682348645174 1104 58-59 0.5797132342345535 1104 60-61 0.5457720911686508 1104 62-63 0.31076215590913137 1104 64-65 0.20174908322702123 1104 66-67 0.21171669936298798 1104 68-69 0.11411135874352851 1104 70-71 0.3076442871338969 1104 72-73 0.2643021742221876 1104 74-75 0.16543679148677626 1104 76-77 -0.006403808412990486 1104 78-79 0.31663871052550263 1104 80-81 0.2816727262182539 1104 82-83 0.2539840387483352 1104 84-85 0.23765515427475492 1104 86-87 0.2118024717766076 1104 88-89 0.1836599749825254 1104 90-91 0.08202199663359266 1104 92-93 0.17290040935564122 1104 94-95 -0.04886102742428022 1104 96-97 -0.06353268969420611 1104 98-99 -0.07756641593137203 1104 100-101 0.004720166832527184 1105 1 0.04012324537637113 1105 2 0.09584655081524573 1105 3 0.11170915748593302 1105 4 0.046020218848198624 1105 5 -0.025254822769873897 1105 6 -0.06527049116943573 1105 7 -0.007087582360668421 1105 8 -0.07856234315407562 1105 9 0.012146459855181035 1105 10-11 -0.023557754162553124 1105 12-13 0.06730526425617 1105 14-15 0.12118073689375564 1105 16-17 0.08047729923068658 1105 18-19 0.019511635046370657 1105 20-21 -0.0965601989563396 1105 22-23 -0.050162160988023174 1105 24-25 0.07325510105705035 1105 26-27 0.012196648145298639 1105 28-29 0.0022879671083160247 1105 30-31 0.024109264147945453 1105 32-33 0.09287447316025066 1105 34-35 -0.0951121308025975 1105 36-37 -0.013656426834636193 1105 38-39 0.10326643221584675 1105 40-41 0.17745096141466377 1105 42-43 0.23630027517307894 1105 44-45 0.2903489096786771 1105 46-47 0.012381857703545052 1105 48-49 0.13480223977227013 1105 50-51 0.26899328623758834 1105 52-53 0.09242792024790702 1105 54-55 0.039939934920404596 1105 56-57 0.08655995676814854 1105 58-59 0.18845867253052262 1105 60-61 0.15389145886061328 1105 62-63 0.12346696711043847 1105 64-65 1.310747366716214E-4 1105 66-67 0.1788119179523946 1105 68-69 -0.0017908345982462492 1105 70-71 0.055261029518646865 1105 72-73 -0.09061701844928649 1105 74-75 -0.03640697480502553 1105 76-77 0.029549867017117037 1105 78-79 0.01610620140209562 1105 80-81 0.00982076747840921 1105 82-83 0.13264503897176638 1105 84-85 0.2376877378749569 1105 86-87 0.23609587313208635 1105 88-89 0.0694451466515531 1105 90-91 0.19033453852679827 1105 92-93 0.11420105357996846 1105 94-95 0.23038973596293033 1105 96-97 0.16095900614254077 1105 98-99 0.2537226843888831 1105 100-101 0.19949561927909087 1106 1 0.13970645996742093 1106 2 0.14589642257357838 1106 3 0.12478268270679393 1106 4 0.01394199143297925 1106 5 0.13485133283001005 1106 6 -0.046194543606027594 1106 7 -0.025807303078821064 1106 8 0.13275142145122487 1106 9 0.08549356729319868 1106 10-11 0.019198372939179364 1106 12-13 0.12522774929464475 1106 14-15 0.05819092498153822 1106 16-17 8.249766316552609E-4 1106 18-19 0.09369587557073089 1106 20-21 -0.013612778038698536 1106 22-23 -0.012891927303215311 1106 24-25 -0.06169253359669824 1106 26-27 -0.018055916687984563 1106 28-29 -0.041830727676519075 1106 30-31 -0.0611091029081976 1106 32-33 -0.38619719968101407 1106 34-35 -0.19842682159769964 1106 36-37 -0.1849748223889307 1106 38-39 -0.10613261309620015 1106 40-41 -0.1024275652311104 1106 42-43 -0.02530381431110129 1106 44-45 -0.0994790327550632 1106 46-47 -0.5385585811679263 1106 48-49 -0.2909600856765806 1106 50-51 -0.16790112812547875 1106 52-53 -0.4829942085637242 1106 54-55 -0.24829211124659878 1106 56-57 -0.2952885043318787 1106 58-59 -0.1917422394102033 1106 60-61 -0.10295700223941395 1106 62-63 -0.1169327194099381 1106 64-65 -0.2147093356366554 1106 66-67 -0.1852341065560097 1106 68-69 -0.5735972609305335 1106 70-71 -0.1256665851864014 1106 72-73 -0.08969082865162648 1106 74-75 -0.140897214189458 1106 76-77 -0.19889840884495413 1106 78-79 -0.15420585331434467 1106 80-81 -0.07044604642869245 1106 82-83 -0.00598954353037584 1106 84-85 -0.10673018745989538 1106 86-87 -0.23610062159575307 1106 88-89 -0.34817739538321035 1106 90-91 -0.14442963930164154 1106 92-93 -0.19686513336788636 1106 94-95 -0.3163828488190603 1106 96-97 -0.24649325946019474 1106 98-99 -0.27471312353360844 1106 100-101 -0.3054203111854292 1107 1 0.13210875162722857 1107 2 0.10087814856218813 1107 3 0.06972193396621407 1107 4 0.08865141818876765 1107 5 0.10250139374338119 1107 6 0.11927580639891033 1107 7 0.12674776590145598 1107 8 0.0961869355930034 1107 9 0.08037355148864833 1107 10-11 0.07030386831306146 1107 12-13 -0.04033314761359463 1107 14-15 0.024416930052161945 1107 16-17 0.10000264611898757 1107 18-19 -0.02387516259067013 1107 20-21 0.026902845417886567 1107 22-23 0.0509806814353837 1107 24-25 0.05084903566345389 1107 26-27 0.0357618700164366 1107 28-29 -0.06598205624653986 1107 30-31 0.14001203238534288 1107 32-33 0.05850333701517485 1107 34-35 -0.1061842217491531 1107 36-37 -0.036354427933687816 1107 38-39 -0.0565211416267104 1107 40-41 0.03207985961496718 1107 42-43 0.0977123853530486 1107 44-45 0.023644176885703416 1107 46-47 -0.07097437254787309 1107 48-49 -0.0025105779224716684 1107 50-51 -0.00723799553188087 1107 52-53 -0.04314616273052252 1107 54-55 0.11701525433240789 1107 56-57 -0.025985550856525208 1107 58-59 -0.014196225120251427 1107 60-61 -0.14142297306684526 1107 62-63 -0.08623731919261957 1107 64-65 0.018523024180275627 1107 66-67 0.04748378221946581 1107 68-69 0.009796437004993663 1107 70-71 0.010577693895619689 1107 72-73 0.23518322883742115 1107 74-75 0.23403281774890417 1107 76-77 0.06554382223073674 1107 78-79 0.31875766451516085 1107 80-81 0.26387781514979025 1107 82-83 0.2893415288742247 1107 84-85 0.13624008022967615 1107 86-87 0.1472873142641049 1107 88-89 0.19893580470897376 1107 90-91 0.24740796894305817 1107 92-93 0.10472602266911935 1107 94-95 0.07229796511528264 1107 96-97 0.08168987576748776 1107 98-99 0.0997394792782913 1107 100-101 0.014992253432133396 1108 1 0.0726785479137817 1108 2 0.055166654914138746 1108 3 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48-49 -0.3140138835012465 2316 50-51 -0.2203365549725902 2316 52-53 -0.22883664331499887 2316 54-55 -0.19651536433732275 2316 56-57 -0.28911830593973775 2316 58-59 -0.311858437306924 2316 60-61 -0.5640892561633493 2316 62-63 -0.4184561278815764 2316 64-65 -0.24205862741498407 2316 66-67 -0.0731318479030918 2316 68-69 -0.10115485320202566 2316 70-71 -0.18442702835977087 2316 72-73 -0.12392529619052794 2316 74-75 -0.12631054992481694 2316 76-77 -0.12090113364059363 2316 78-79 -0.19175342464318845 2316 80-81 -0.14237727348749019 2316 82-83 -0.15426008646279143 2316 84-85 -0.2819671996426578 2316 86-87 -0.3164680186277593 2316 88-89 -0.3787778637778416 2316 90-91 -0.40414657582066127 2316 92-93 -0.31155707382772135 2316 94-95 -0.3280054533633887 2316 96-97 -0.2985683827515686 2316 98-99 -0.5767263196513355 2316 100-101 -0.6814589987797319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 11.0 15 20.0 16 100.0 17 295.0 18 665.0 19 1099.0 20 1578.0 21 2564.0 22 4096.0 23 4210.0 24 2996.0 25 2093.0 26 1583.0 27 1552.0 28 1826.0 29 2069.0 30 2119.0 31 2434.0 32 3047.0 33 4031.0 34 6057.0 35 10496.0 36 22222.0 37 77634.0 38 228114.0 39 107344.0 40 83.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23076907373497 20.915629102029403 14.39994773899712 22.45365408523851 2 14.79189780069177 22.16622887163088 41.097941003719896 21.943932323957448 3 16.582642993200608 29.210870868666106 32.31464826303488 21.891837875098403 4 11.360123017184065 19.39070600279807 41.51014198369288 27.739028996324983 5 11.649538990531003 39.40004527498434 36.30829409161454 12.642121642870109 6 26.744408958718275 39.85332566515343 20.20748952763196 13.194775848496343 7 24.185969678058807 34.924684605313075 24.593647647133203 16.29569806949492 8 24.40316679514947 34.57411010364279 22.889109145120305 18.133613956087434 9 26.58431530903173 15.32412335980487 22.153494120382266 35.938067210781135 10-11 22.348155761943804 27.37560621448878 31.21010405067525 19.066133972892167 12-13 24.331991401849336 26.225889080593383 29.672695161296904 19.769424356260377 14-15 21.89234772748594 27.01605831079786 28.38185088653133 22.70974307518487 16-17 20.044744645530226 29.70542768457676 29.194351651309912 21.0554760185831 18-19 19.76525294963442 28.56847128886532 31.7099211731234 19.95635458837686 20-21 21.386281176132275 27.584286964200857 31.624003964765794 19.405427894901077 22-23 21.09820164865868 27.950005914287697 30.77081523357358 20.18097720348005 24-25 20.629035481647353 28.194939001260355 31.189506014218765 19.986519502873527 26-27 20.3851715283882 28.27643592949195 31.312471065227896 20.025921476891952 28-29 20.430702902895554 27.699566421529635 31.666931789908187 20.202798885666624 30-31 21.015911473310247 27.471968315733232 31.40731495417447 20.104805256782058 32-33 20.373701405968944 27.99833992062618 31.380088836680002 20.24786983672487 34-35 20.914144936757907 28.016388695144983 31.188894191353718 19.88057217674339 36-37 20.959501710118147 27.648643632588715 30.78844910537469 20.603405551918453 38-39 20.475129963759027 28.17292063084174 31.204966461294354 20.146982944104877 40-41 20.632400507405098 27.7295457419168 31.5062263173566 20.131827433321504 42-43 20.792902039001667 27.730871358124396 30.803139874943408 20.673086727930528 44-45 20.836647373852323 28.06849560915124 30.684548209602358 20.410308807394085 46-47 21.116623184296575 27.868561532483266 30.038838357773894 20.975976925446265 48-49 20.92219337549533 28.069712092443677 30.03929998307266 20.96879454898834 50-51 21.122266681350414 28.138345386243774 29.538502012897226 21.200885919508583 52-53 21.02723019631356 28.468831703845105 29.626910416896102 20.877027682945233 54-55 21.432562844405286 28.38021935889121 29.314880755723603 20.872337040979897 56-57 21.319273643894622 28.710093853627498 28.980825471409517 20.989807031068363 58-59 20.853880384551065 28.48973565173411 29.63037741313135 21.026006550583475 60-61 21.597820190162302 28.807936889936737 29.089284913056424 20.504958006844536 62-63 21.247033555623318 28.761584048152518 29.184407542289996 20.806974853934165 64-65 21.020491205598393 28.707472309388343 29.265006878673255 21.007029606340005 66-67 21.14675091320336 28.955633919156774 28.806441678377016 21.09117348926285 68-69 21.150231941075038 28.712867248533353 29.060294482056054 21.07660632833556 70-71 21.22014292240423 28.380946942504732 29.26554444299419 21.13336569209685 72-73 21.33954829683351 28.298444695526616 28.878063802970296 21.483943204669583 74-75 21.39934145518214 28.483134094123574 28.59744637978804 21.520078070906248 76-77 20.982993707151575 28.510855698897757 29.022971803021914 21.483178790928754 78-79 20.92383213212111 28.60261697033475 28.952477678662476 21.52107321888167 80-81 21.216691343522225 28.77137811060942 28.4923868841493 21.51954366171906 82-83 21.277873630026633 28.72844853957882 28.639428312714905 21.35424951767964 84-85 21.159078023730572 28.441911497783163 28.670835219787165 21.7281752586991 86-87 20.897303073810612 28.730197252496247 28.904465509365007 21.468034164328134 88-89 21.27506615476391 28.44292845088232 28.760063426995224 21.52194196735855 90-91 21.1752541367 28.566708780602706 28.891383774204044 21.366653308493248 92-93 21.375663317956185 28.63004702878423 28.781371217405137 21.21291843585445 94-95 21.126131454747927 28.414046151524175 28.865608262730014 21.594214130997884 96-97 21.373725878883544 28.3938834028772 28.740786967357213 21.491603750882042 98-99 20.988481414860768 28.52053073594133 28.725695336686126 21.765292512511778 100-101 21.104829729696657 28.440279970143045 28.956964379672794 21.4979259204875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 173.0 1 109.0 2 53.0 3 60.5 4 51.0 5 45.0 6 77.5 7 162.5 8 299.0 9 372.5 10 307.5 11 281.0 12 306.5 13 353.0 14 415.5 15 451.0 16 524.5 17 575.5 18 572.0 19 607.0 20 688.0 21 806.5 22 968.5 23 1129.5 24 1412.5 25 1812.5 26 2409.0 27 3331.5 28 4241.0 29 5528.5 30 7208.0 31 8806.0 32 11209.0 33 13955.5 34 16243.0 35 18497.0 36 21075.5 37 23223.0 38 22808.0 39 21728.0 40 20999.0 41 19702.0 42 18929.5 43 18956.5 44 18495.5 45 18461.0 46 18030.0 47 16384.0 48 16072.0 49 15985.5 50 15151.0 51 14095.5 52 12886.5 53 11779.0 54 10577.0 55 9379.5 56 8658.5 57 7598.5 58 6124.0 59 4841.0 60 3965.0 61 3262.0 62 2341.0 63 1489.5 64 933.5 65 676.5 66 570.0 67 425.5 68 324.0 69 202.0 70 83.0 71 39.0 72 26.5 73 15.5 74 17.0 75 21.0 76 14.0 77 7.0 78 5.5 79 6.5 80 6.0 81 4.5 82 3.5 83 1.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09972712700219032 2 4.078819100294083E-4 3 0.0 4 0.0 5 2.0394095501470415E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.004690641965338195 20-21 0.003874878145279379 22-23 0.0 24-25 0.0 26-27 0.0022433505051617456 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.004282760055308787 38-39 0.0018354685951323371 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.020598036456485117 48-49 0.001427586685102929 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.004078819100294083 62-63 0.01274630968841901 64-65 0.011114782048301377 66-67 0.006322169605455828 68-69 0.003976848622786731 70-71 4.078819100294083E-4 72-73 0.0032630552802352664 74-75 0.00295714384771321 76-77 0.003976848622786731 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 4.078819100294083E-4 88-89 0.0021413800276543936 90-91 3.059114325220562E-4 92-93 0.0 94-95 0.008157638200588166 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 490338.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.95829404438077 #Duplication Level Percentage of deduplicated Percentage of total 1 63.59205848905118 26.046222304820187 2 16.0524341428007 13.149606354977767 3 7.332036460332633 9.009231158592742 4 3.886038987124167 6.366621100102371 5 2.385021182099507 4.884319943925411 6 1.526248150006033 3.750751230758356 7 1.06848907036704 3.0634442669110205 8 0.7206461871131915 2.3613150746995157 9 0.5529202798172037 2.0382004263468896 >10 2.64341307589672 18.86985025079821 >50 0.1757368146692635 4.926938636584169 >100 0.06294298519619121 4.111041393209795 >500 5.035438815695297E-4 0.18768111512672017 >1k 0.0015106316447085892 1.2347767431468941 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2599 0.5300425420832161 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2202 0.4490779829423785 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 0.24187397264743912 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 910 0.18558626906338077 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.0394095501470415E-4 0.0 4 0.0 0.0 0.0 2.0394095501470415E-4 0.0 5 0.0 0.0 0.0 2.0394095501470415E-4 0.0 6 0.0 0.0 0.0 2.0394095501470415E-4 0.0 7 0.0 0.0 0.0 2.0394095501470415E-4 0.0 8 0.0 0.0 0.0 2.0394095501470415E-4 0.0 9 0.0 0.0 0.0 2.0394095501470415E-4 0.0 10-11 0.0 0.0 0.0 2.0394095501470415E-4 0.0 12-13 0.0 0.0 0.0 2.0394095501470415E-4 0.0 14-15 0.0 0.0 0.0 2.0394095501470415E-4 0.0 16-17 0.0 0.0 0.0 6.118228650441124E-4 0.0 18-19 0.0 0.0 0.0 8.157638200588166E-4 0.0 20-21 0.0 0.0 0.0 8.157638200588166E-4 0.0 22-23 0.0 0.0 0.0 0.0017334981176249853 0.0 24-25 0.0 0.0 0.0 0.0032630552802352664 0.0 26-27 0.0 0.0 0.0 0.0035689667127573226 0.0 28-29 0.0 0.0 0.0 0.0062201991279484765 0.0 30-31 0.0 0.0 0.0 0.010197047750735207 0.0 32-33 0.0 0.0 0.0 0.01672315831120574 0.0 34-35 0.0 0.0 0.0 0.02763399940449241 0.0 36-37 0.0 0.0 0.0 0.044968980580742265 0.0 38-39 0.0 0.0 0.0 0.06954386566001411 0.0 40-41 0.0 0.0 0.0 0.09422072121679331 0.0 42-43 0.0 0.0 0.0 0.10890446997785203 0.0 44-45 0.0 0.0 0.0 0.12685127401914598 0.0 46-47 0.0 0.0 0.0 0.15346556864856487 0.0 48-49 0.0 0.0 0.0 0.18069168614302789 0.0 50-51 0.0 0.0 0.0 0.20567445313232913 0.0 52-53 0.0 0.0 0.0 0.23035130868910833 0.0 54-55 0.0 0.0 0.0 0.2544163413808434 0.0 56-57 0.0 0.0 0.0 0.2840897503354829 0.0 58-59 0.0 0.0 0.0 0.31478286406519584 0.0 60-61 0.0 0.0 0.0 0.34343656824476176 0.0 62-63 0.0 0.0 0.0 0.3750474162720409 0.0 64-65 0.0 0.0 0.0 0.4040070318841289 0.0 66-67 0.0 0.0 0.0 0.4383710828041065 0.0 68-69 0.0 0.0 0.0 0.4776297166444371 0.0 70-71 0.0 0.0 0.0 0.524536136297819 0.0 72-73 0.0 0.0 0.0 0.5695051168785613 0.0 74-75 0.0 0.0 0.0 0.6177371527395388 0.0 76-77 0.0 0.0 0.0 0.6630120447528032 0.0 78-79 0.0 0.0 0.0 0.717974132129266 0.0 80-81 0.0 0.0 0.0 0.7715086328206258 0.0 82-83 0.0 0.0 0.0 0.830345598342368 0.0 84-85 0.0 0.0 0.0 0.8939751763069557 0.0 86-87 0.0 0.0 0.0 0.952914112306205 0.0 88-89 0.0 0.0 0.0 1.020928420803609 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 965 0.0 65.072876 1 ACCGCGA 20 5.3908146E-4 47.494595 24-25 GTATCAA 2040 0.0 46.17303 1 TCAACGC 2450 0.0 37.995678 4 CGCGAGG 25 0.001616604 37.995674 26-27 ATCAACG 2480 0.0 37.727562 3 CAACGCA 2485 0.0 37.460526 5 TATCAAC 2615 0.0 35.96149 2 AACGCAG 2595 0.0 35.8726 6 TCCGTTA 30 0.0039508995 31.666294 18-19 TCCCGGT 30 0.0039508995 31.666294 16-17 CCGGTGA 30 0.0039508995 31.666294 18-19 ACGCAGA 2940 0.0 31.663061 7 CGCAGAG 2960 0.0 31.28867 8 GCAGAGT 3375 0.0 27.159872 9 CGGTGAG 35 0.008391462 27.142536 36-37 GACCGCG 35 0.008395638 27.139769 24-25 TACGAGA 35 0.008395638 27.139769 22-23 CCGCGAG 35 0.008395638 27.139769 26-27 GTACATG 2640 0.0 27.029669 1 >>END_MODULE