##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031436_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36633 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.61810389539486 34.0 33.0 34.0 31.0 34.0 2 32.69412824502498 34.0 33.0 34.0 31.0 34.0 3 32.799170147135094 34.0 33.0 34.0 31.0 34.0 4 35.77795976305517 37.0 37.0 37.0 35.0 37.0 5 35.80796003603309 37.0 37.0 37.0 35.0 37.0 6 35.91005377664947 37.0 37.0 37.0 35.0 37.0 7 35.94161002374908 37.0 37.0 37.0 35.0 37.0 8 36.03472279092622 37.0 37.0 37.0 35.0 37.0 9 37.912756258018725 39.0 39.0 39.0 37.0 39.0 10-11 38.00305735266017 39.0 39.0 39.0 37.0 39.0 12-13 38.05168836841099 39.0 39.0 39.0 37.0 39.0 14-15 39.53225234078563 41.0 40.0 41.0 38.0 41.0 16-17 39.47511806294871 41.0 40.0 41.0 37.5 41.0 18-19 39.39611825403325 41.0 40.0 41.0 36.0 41.0 20-21 39.3048617366855 41.0 40.0 41.0 35.0 41.0 22-23 39.17080228209538 41.0 40.0 41.0 35.0 41.0 24-25 38.99605546911255 41.0 40.0 41.0 35.0 41.0 26-27 38.82416127535282 41.0 39.0 41.0 35.0 41.0 28-29 38.67698523189473 41.0 39.0 41.0 35.0 41.0 30-31 38.35582671361887 41.0 37.5 41.0 35.0 41.0 32-33 37.968116179401086 40.5 36.0 41.0 35.0 41.0 34-35 37.8111675265471 40.0 35.0 41.0 35.0 41.0 36-37 37.62077907897251 40.0 35.0 41.0 35.0 41.0 38-39 37.40497638741026 40.0 35.0 41.0 35.0 41.0 40-41 37.27042557257118 40.0 35.0 41.0 35.0 41.0 42-43 37.26848742936697 40.0 35.0 41.0 35.0 41.0 44-45 37.1081129036661 40.0 35.0 41.0 34.5 41.0 46-47 36.79930663609314 40.0 35.0 41.0 32.5 41.0 48-49 36.48457674774111 40.0 35.0 41.0 30.5 41.0 50-51 35.568244479021644 39.0 35.0 40.0 27.0 40.5 52-53 35.63669369148036 39.5 35.0 40.0 27.0 40.5 54-55 35.98033194114596 40.0 35.0 41.0 26.5 41.0 56-57 35.9858051483635 40.0 35.0 41.0 25.5 41.0 58-59 35.97121447874867 40.0 35.0 41.0 25.5 41.0 60-61 35.79422924685393 40.0 35.0 41.0 23.5 41.0 62-63 35.596593235607244 39.5 35.0 41.0 22.5 41.0 64-65 35.40702372178091 39.0 35.0 41.0 22.0 41.0 66-67 35.16205333988481 39.0 35.0 41.0 21.5 41.0 68-69 34.84190483989845 37.5 35.0 41.0 20.5 41.0 70-71 34.476305516883684 37.0 35.0 40.0 19.0 41.0 72-73 34.15318155761199 37.0 35.0 39.0 18.5 41.0 74-75 33.76549832118582 36.0 35.0 39.0 16.0 41.0 76-77 33.41058608358584 36.0 35.0 38.5 15.0 41.0 78-79 33.029386072666725 35.0 35.0 37.0 14.0 39.5 80-81 32.6547921273169 35.0 35.0 37.0 10.5 39.0 82-83 32.29585346545464 35.0 35.0 36.5 10.0 39.0 84-85 31.9549040482625 35.0 35.0 36.0 10.0 37.5 86-87 31.775803237518083 35.0 35.0 36.0 10.0 37.0 88-89 31.52918133922966 35.0 34.5 35.5 10.0 37.0 90-91 31.291567712172085 35.0 34.0 35.0 10.0 36.0 92-93 31.18723555264379 35.0 34.0 35.0 10.0 36.0 94-95 31.03398575055278 35.0 34.0 35.0 10.0 36.0 96-97 30.895954467283595 35.0 34.0 35.0 10.0 36.0 98-99 30.76188136379767 35.0 34.0 35.0 8.0 35.5 100-101 30.45989954412688 35.0 33.5 35.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6640191467099967 1101 2 0.8599113669148863 1101 3 0.694653546455207 1101 4 0.9955398576266106 1101 5 1.0898946775295286 1101 6 1.0646814573258894 1101 7 0.9134356705924489 1101 8 0.8331703607484826 1101 9 0.8891684070595147 1101 10-11 0.8114848281588749 1101 12-13 0.7187564557031081 1101 14-15 1.0379371107310078 1101 16-17 0.7968916782687643 1101 18-19 0.620328834398876 1101 20-21 0.8967962430994447 1101 22-23 0.5891602641452636 1101 24-25 0.8595394144171635 1101 26-27 0.875309222977549 1101 28-29 0.6989526888229562 1101 30-31 0.9629286985746077 1101 32-33 0.6517767176274418 1101 34-35 1.4441622888163508 1101 36-37 1.2358746616870206 1101 38-39 1.504122859281786 1101 40-41 1.7779946169271597 1101 42-43 1.2014534582698957 1101 44-45 1.0253264373466138 1101 46-47 1.3899235677279478 1101 48-49 1.5725969443803294 1101 50-51 2.2252006635640242 1101 52-53 2.0230373887724653 1101 54-55 1.9474829971047996 1101 56-57 1.3603852825824276 1101 58-59 1.0948077624946464 1101 60-61 1.6810914044054002 1101 62-63 2.0823968890022897 1101 64-65 1.4990504037898518 1101 66-67 0.8489595210803742 1101 68-69 0.7330179924445801 1101 70-71 0.2602595816880253 1101 72-73 0.29237345463331366 1101 74-75 0.32450783121507243 1101 76-77 -0.24165193862631185 1101 78-79 0.04014883714410189 1101 80-81 -0.07843050690657805 1101 82-83 -0.0511704143819216 1101 84-85 0.0019416560913683156 1101 86-87 0.19699445007236704 1101 88-89 -0.2486555712425229 1101 90-91 0.4048917509569243 1101 92-93 0.6389331241775587 1101 94-95 0.7928580049203049 1101 96-97 0.6253758062805126 1101 98-99 0.9356673139609377 1101 100-101 1.1070713528482266 1102 1 -0.1377049912210353 1102 2 -0.2275270567304304 1102 3 -0.21913955699307053 1102 4 -0.06603649705319015 1102 5 -0.07759300719953188 1102 6 -0.2789146017874131 1102 7 -0.39198304861937316 1102 8 -0.3343173239805779 1102 9 -0.03447691313753154 1102 10-11 0.18032719269088915 1102 12-13 0.5371062093976917 1102 14-15 0.8815331698443032 1102 16-17 0.8233695107810775 1102 18-19 1.0156490314432105 1102 20-21 1.2342346667447615 1102 22-23 1.0805395744900892 1102 24-25 1.2782586262398183 1102 26-27 0.6259249865243461 1102 28-29 1.132450225768764 1102 30-31 1.0903917527617963 1102 32-33 1.4559639097456625 1102 34-35 1.4841869193582227 1102 36-37 0.9784854991254477 1102 38-39 1.2282607903162628 1102 40-41 1.8444970799813518 1102 42-43 0.9280544434915754 1102 44-45 0.6263116590214892 1102 46-47 1.5740368682205528 1102 48-49 0.6914393089123436 1102 50-51 0.2325898261255972 1102 52-53 0.8629388666049778 1102 54-55 1.1894780709964294 1102 56-57 1.0537350362770113 1102 58-59 1.0061378117557283 1102 60-61 1.7531357393807596 1102 62-63 1.748653046637756 1102 64-65 1.1536070540361507 1102 66-67 1.9850432649227372 1102 68-69 1.4873283372721673 1102 70-71 1.3193728821806232 1102 72-73 1.187077888130851 1102 74-75 0.9568969937766525 1102 76-77 1.2146288495510333 1102 78-79 1.2439665711342442 1102 80-81 0.6413970793003188 1102 82-83 1.4863911619727617 1102 84-85 1.7925820501800374 1102 86-87 1.1939156323383813 1102 88-89 1.5635365469840785 1102 90-91 2.1518129332229314 1102 92-93 1.831667114325338 1102 94-95 1.6918727832454294 1102 96-97 2.1210654614529254 1102 98-99 2.028647609527443 1102 100-101 2.0159383479221127 1103 1 0.1109888436796922 1103 2 0.23328799029150815 1103 3 0.19465354645520705 1103 4 0.3613407234274746 1103 5 0.47517606281091673 1103 6 0.494335136979565 1103 7 0.3896261467829305 1103 8 0.21845174602987072 1103 9 0.16406018195129235 1103 10-11 0.31094370261774174 1103 12-13 0.6175659795126407 1103 14-15 0.8355561583500446 1103 16-17 0.7617185180956056 1103 18-19 0.8724933365633802 1103 20-21 0.9400862863894872 1103 22-23 1.142190567175568 1103 24-25 1.1387601936379426 1103 26-27 1.3401360628043903 1103 28-29 1.2855327754030412 1103 30-31 1.2199633306092394 1103 32-33 1.3216901375408554 1103 34-35 1.1503311199851822 1103 36-37 0.8624980383103988 1103 38-39 1.3071531623120833 1103 40-41 1.6005054394379812 1103 42-43 1.6971244539408872 1103 44-45 2.0296554416756223 1103 46-47 2.2443607971651716 1103 48-49 2.397813394596774 1103 50-51 2.378880317243677 1103 52-53 2.183210548945631 1103 54-55 2.162309836931641 1103 56-57 2.3766190488161882 1103 58-59 2.2506519183388107 1103 60-61 2.4521953005092954 1103 62-63 2.4189769755823747 1103 64-65 2.0721023518417994 1103 66-67 1.8711456682665215 1103 68-69 2.0625634469900334 1103 70-71 2.323030144458585 1103 72-73 2.269105056364914 1103 74-75 1.90621778792503 1103 76-77 1.8389757670013935 1103 78-79 1.5720752440705112 1103 80-81 1.6602058567297817 1103 82-83 0.9623577241462158 1103 84-85 0.8093009634506743 1103 86-87 0.6683147963927212 1103 88-89 0.4109764633895061 1103 90-91 0.4292424003075723 1103 92-93 0.2736733839178207 1103 94-95 0.19761990968221 1103 96-97 0.1215879274926337 1103 98-99 -0.186085932792313 1103 100-101 -0.005482759705888185 1104 1 1.0691915605031 1104 2 0.9793694949937048 1104 3 0.9877569947310718 1104 4 0.8649979857054291 1104 5 0.8534414755590873 1104 6 0.9279819499367292 1104 7 0.9183617789668332 1104 8 0.8036137105021837 1104 9 0.551729983414198 1104 10-11 0.6803271926908891 1104 12-13 0.5543475887080334 1104 14-15 0.7091193767408512 1104 16-17 0.8923350280224582 1104 18-19 0.9811662728225272 1104 20-21 0.872165701227523 1104 22-23 0.8564016434556123 1104 24-25 1.0886034538260247 1104 26-27 1.1086836072139974 1104 28-29 1.3393467774929064 1104 30-31 1.383495201037661 1104 32-33 1.36975701319394 1104 34-35 1.346255884875461 1104 36-37 1.8060717060219957 1104 38-39 1.8489504454886827 1104 40-41 2.1720832868779 1104 42-43 1.5659854779743227 1104 44-45 1.3332082107456316 1104 46-47 1.7636920406343393 1104 48-49 1.0362668951192475 1104 50-51 0.9222449985393908 1104 52-53 1.190525073501533 1104 54-55 1.430857381341255 1104 56-57 1.3295971052425202 1104 58-59 0.575103328997109 1104 60-61 0.8393426359324891 1104 62-63 0.45554959836189113 1104 64-65 0.36050360576029306 1104 66-67 0.5367674028537692 1104 68-69 0.6769835096859609 1104 70-71 0.8710970201116695 1104 72-73 1.9284571984756766 1104 74-75 1.9913797523973429 1104 76-77 1.2146288495510333 1104 78-79 1.261207950444593 1104 80-81 1.3310522517141123 1104 82-83 1.6070808171451745 1104 84-85 0.9477544639731477 1104 86-87 0.8490880461314845 1104 88-89 0.08077792629442015 1104 90-91 0.18629569184362182 1104 92-93 0.3144257350149964 1104 94-95 0.3298038177281839 1104 96-97 0.6383068407632706 1104 98-99 1.25278554056192 1104 100-101 0.08490386516349346 1105 1 -0.005335691999682979 1105 2 0.19516482313608208 1105 3 0.19465354645520705 1105 4 -0.20952926679735384 1105 5 0.29726794941893075 1105 6 -0.12541070857272985 1105 7 0.08855087796572292 1105 8 0.26635008425078865 1105 9 0.4582928310226464 1105 10-11 0.04017146410357242 1105 12-13 -7.135903798385357E-4 1105 14-15 -0.030035239499412114 1105 16-17 -0.14492859822893678 1105 18-19 0.14913067771684752 1105 20-21 0.21476859332985754 1105 22-23 0.27464413511300734 1105 24-25 -0.19115182982247347 1105 26-27 -0.17941427932660048 1105 28-29 -0.05219938490976972 1105 30-31 -0.09870724612585491 1105 32-33 -0.3171172915891489 1105 34-35 0.02423141324031519 1105 36-37 0.22320185062711317 1105 38-39 -0.31845778587950946 1105 40-41 0.013017658401814458 1105 42-43 -0.9690534541725029 1105 44-45 -0.2073924566625962 1105 46-47 0.08404799169107946 1105 48-49 -1.0454906132233575 1105 50-51 0.10077670043038722 1105 52-53 -0.3456262056975774 1105 54-55 0.0621143335103298 1105 56-57 -1.2421492796295581 1105 58-59 -0.8798466153855387 1105 60-61 -0.552203526470187 1105 62-63 -0.628432604085269 1105 64-65 -0.5833228680995504 1105 66-67 -1.075579649426544 1105 68-69 -3.0855303946521957 1105 70-71 -3.428680510477882 1105 72-73 -2.857972167486505 1105 74-75 -3.5781419383701802 1105 76-77 -2.799831662128611 1105 78-79 -2.8394594577867736 1105 80-81 -2.687301474648514 1105 82-83 -2.4618847000962063 1105 84-85 -2.8920675604984893 1105 86-87 -3.860033199697213 1105 88-89 -3.437019626542064 1105 90-91 -3.5150391246191113 1105 92-93 -3.465329548633502 1105 94-95 -3.555068262360802 1105 96-97 -3.9512370969452952 1105 98-99 -2.884033146868557 1105 100-101 -2.6003019190411756 1106 1 -0.5253747926839409 1106 2 -0.06974231273878928 1106 3 -0.16898281718115982 1106 4 0.20982557191232587 1106 5 0.2630548506897057 1106 6 0.0700927127371429 1106 7 0.23811099526777468 1106 8 -0.6603361327580046 1106 9 0.10345412134523002 1106 10-11 0.3563982480722885 1106 12-13 0.16302052496718034 1106 14-15 0.38101070380459134 1106 16-17 0.7617185180956056 1106 18-19 0.6149175789876224 1106 20-21 0.697662043965245 1106 22-23 0.5664329914179973 1106 24-25 0.09330564818339582 1106 26-27 0.6280148506831722 1106 28-29 0.5734115632818302 1106 30-31 1.2654178760637862 1106 32-33 1.7762355920863087 1106 34-35 1.4836644533185108 1106 36-37 1.4534071292194852 1106 38-39 2.216244071402997 1106 40-41 2.070202409134957 1106 42-43 2.0001547569711917 1106 44-45 2.544806956827138 1106 46-47 2.8807244335288047 1106 48-49 2.7462982430816254 1106 50-51 2.4394863778497395 1106 52-53 2.3498772156122953 1106 54-55 2.025946200568008 1106 56-57 1.6190432912404376 1106 58-59 2.3264094940963744 1106 60-61 1.5582559065698902 1106 62-63 1.2523103089157033 1106 64-65 1.0417993215387682 1106 66-67 1.0075093046301618 1106 68-69 0.7443816288082132 1106 70-71 1.0048483262767647 1106 72-73 1.0569838442437032 1106 74-75 0.6031874848947254 1106 76-77 1.2026121306377604 1106 78-79 1.4660146380099022 1106 80-81 0.9329331294570622 1106 82-83 0.9017516635401535 1106 84-85 1.566876721026432 1106 86-87 1.4410420691199946 1106 88-89 1.6534007058137554 1106 90-91 1.9746969457621155 1106 92-93 2.122158232402665 1106 94-95 1.8188320308943275 1106 96-97 0.45492126082596585 1106 98-99 0.813914067207687 1106 100-101 1.3884566342335027 1107 1 -0.2076024749116243 1107 2 -0.24337048636696323 1107 3 -0.10264375084209121 1107 4 -0.2766609145741583 1107 5 -0.0375184498835992 1107 6 0.31661195925639163 1107 7 0.43467119182797376 1107 8 -0.2672157396376136 1107 9 -0.03168101378990684 1107 10-11 0.2646701563442022 1107 12-13 0.15360201554867814 1107 14-15 -0.1599393371454454 1107 16-17 0.3350190913961768 1107 18-19 0.23326319733323686 1107 20-21 0.14196998827318907 1107 22-23 0.7986197236047232 1107 24-25 1.0699641248418672 1107 26-27 0.697220419888744 1107 28-29 0.9730839629542274 1107 30-31 0.5647626754085806 1107 32-33 0.6488809647316884 1107 34-35 0.41568738534144245 1107 36-37 0.852670028482386 1107 38-39 1.1392579944169157 1107 40-41 0.909268748201292 1107 42-43 1.2323414891579247 1107 44-45 1.9403843524045286 1107 46-47 1.3913714441758174 1107 48-49 1.1676741644575444 1107 50-51 1.601648540011901 1107 52-53 1.8936937594288423 1107 54-55 1.5058806805024858 1107 56-57 1.1296903018874431 1107 58-59 0.7936494613363507 1107 60-61 0.608214956528947 1107 62-63 0.9173389739443749 1107 64-65 0.7109229906624321 1107 66-67 0.9440367411575892 1107 68-69 0.675585560012145 1107 70-71 0.6637344851629265 1107 72-73 0.3604236476835041 1107 74-75 0.6502800319872719 1107 76-77 0.382382810408437 1107 78-79 0.7751874471827094 1107 80-81 0.918191114715043 1107 82-83 -0.0024252406367466506 1107 84-85 0.29537794952766205 1107 86-87 0.6077087357866588 1107 88-89 0.81842937084242 1107 90-91 0.7142546853198546 1107 92-93 1.1471377573821933 1107 94-95 0.7561784682407655 1107 96-97 0.7571325630372705 1107 98-99 0.9969607502543703 1107 100-101 1.761511507288379 1108 1 0.8140191467099953 1108 2 0.8491970812006002 1108 3 0.5946535464552056 1108 4 1.1098255719123244 1108 5 1.0327518203866717 1108 6 0.8753957430401726 1108 7 0.9062928134495962 1108 8 0.7760275036056257 1108 9 0.8034541213452329 1108 10-11 0.8704133995874415 1108 12-13 0.6633993128459679 1108 14-15 0.6397228250167188 1108 16-17 1.002248821125903 1108 18-19 1.0721145486845884 1108 20-21 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-1.1298317429234075 2316 86-87 -1.03884298835127 2316 88-89 -0.7468082806021314 2316 90-91 -0.908531894363275 2316 92-93 0.21959814880809958 2316 94-95 -0.4288168719269869 2316 96-97 -0.6806586764781066 2316 98-99 -0.9196282525415285 2316 100-101 -0.6909582038020226 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 4.0 4 14.0 5 3.0 6 6.0 7 54.0 8 100.0 9 104.0 10 62.0 11 49.0 12 56.0 13 87.0 14 135.0 15 159.0 16 196.0 17 169.0 18 213.0 19 273.0 20 348.0 21 463.0 22 512.0 23 367.0 24 285.0 25 209.0 26 194.0 27 189.0 28 243.0 29 265.0 30 271.0 31 307.0 32 355.0 33 457.0 34 817.0 35 3155.0 36 2994.0 37 4772.0 38 12682.0 39 6038.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.060018568073836 24.378788706242148 16.59385069084157 17.967342034842446 2 14.600573535436295 25.83640584459921 40.92585006145023 18.63717055851427 3 14.929815937517068 34.91725380960183 34.144409853077725 16.008520399803373 4 11.116270177259443 23.133859558080463 41.119274574604646 24.630595690055447 5 9.208656683790577 41.6876161329107 36.81549896163515 12.28822822166357 6 21.122527052136846 42.84894523991693 21.395780959667725 14.6327467482785 7 20.16671221645258 39.36321399289424 27.313473626673957 13.15660016397923 8 23.17826491007489 35.628382441371016 23.77138796260865 17.421964685945444 9 25.48719490529423 18.714297428048212 24.899554486566267 30.898953180091286 10-11 20.60736045463239 31.385754487582307 30.271030846151742 17.735854211633562 12-13 23.009900990099013 31.556162512803006 30.299761010583815 15.134175486514167 14-15 20.67806337883819 31.959988521297095 30.527883682477214 16.834064417387502 16-17 16.24327351197793 34.940861536780574 31.39118795924499 17.424676991996506 18-19 16.134889753566796 31.550276469383576 36.0352242473889 16.27960952966073 20-21 17.102442620936362 31.74588003986838 36.293879111426655 14.857798227768598 22-23 17.488250081976172 31.37637993223303 35.419171494152366 15.71619849163843 24-25 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34.385763490241104 16.27576403695807 50-51 15.701202843083653 33.02351011481684 34.15800984144341 17.117277200656098 52-53 16.295253199168762 33.59810784206497 34.02466367713004 16.081975281636225 54-55 17.109943632682096 33.133585070869586 32.959831445301816 16.7966398511465 56-57 16.858588621444202 33.637855579868706 32.5164113785558 16.98714442013129 58-59 16.24085845123368 32.98885927141002 33.56161574738569 17.20866652997061 60-61 16.659825972746674 33.24850872872544 33.24440431237345 16.847260986154435 62-63 16.12232332215913 33.33515769309708 33.62249435588698 16.92002462885681 64-65 16.53917208347588 32.65138556277797 34.09784468012316 16.71159767362299 66-67 16.945746818486267 33.23947127410911 32.83212816271854 16.982653744686086 68-69 16.670311645708036 32.773373428102786 33.648168398031714 16.908146528157463 70-71 16.792432195975504 33.027121609798776 33.48233814523184 16.698108048993877 72-73 17.17363664948665 32.3717992535579 33.13601378046946 17.318550316485982 74-75 16.949685534591193 32.804211101996174 32.95460760185945 17.291495761553186 76-77 16.740943267259055 33.196172248803826 33.589883800410114 16.473000683527 78-79 17.02380789585242 32.483214134314274 33.384386068073354 17.10859190175995 80-81 16.901138603021675 33.23297569520473 32.421447339610246 17.444438362163346 82-83 17.528440593042834 33.25940832614618 32.23677906005722 16.975372020753763 84-85 17.246709896303592 32.723193521026566 32.25259241018906 17.77750417248078 86-87 16.937789878370797 32.841252685009096 32.863143205045766 17.357814231574338 88-89 17.74628233716842 32.36521334664897 32.84402916672367 17.044475149458936 90-91 17.631935232416613 32.95543125965838 32.48088836617754 16.931745141747466 92-93 17.524814743922782 32.69803943014957 32.770501216811134 17.006644609116513 94-95 17.56712418658063 32.31257177229726 32.84026904358287 17.280034997539236 96-97 18.379146789868642 32.09037849068467 32.22980084473544 17.300673874711244 98-99 18.065486362704217 32.085583430172946 32.33030282315948 17.518627383963363 100-101 18.144648497859947 32.01876136689959 32.89939695606394 16.937193179176525 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1484.0 1 854.5 2 215.5 3 189.5 4 133.5 5 131.5 6 258.0 7 422.0 8 590.0 9 620.0 10 392.5 11 209.0 12 208.0 13 211.5 14 171.0 15 121.5 16 83.0 17 67.0 18 69.5 19 74.0 20 79.5 21 91.0 22 118.0 23 145.0 24 154.5 25 182.5 26 258.0 27 371.0 28 444.5 29 583.5 30 781.5 31 921.0 32 1072.0 33 1262.5 34 1482.5 35 1631.5 36 1974.5 37 2315.5 38 2183.0 39 1917.0 40 1557.5 41 1176.5 42 1026.0 43 996.5 44 963.5 45 978.5 46 874.5 47 672.0 48 638.5 49 552.5 50 491.5 51 419.5 52 266.0 53 236.0 54 234.0 55 216.5 56 185.5 57 149.0 58 151.0 59 162.0 60 158.0 61 148.5 62 141.0 63 112.0 64 72.5 65 43.5 66 26.5 67 28.5 68 28.0 69 32.0 70 30.0 71 22.0 72 25.5 73 19.5 74 13.0 75 16.5 76 11.5 77 4.0 78 4.0 79 5.0 80 3.0 81 2.0 82 2.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030027570769524746 2 0.049136024895585946 3 0.04094668741298829 4 0.054595583217317716 5 0.10100182895203778 6 0.10100182895203778 7 0.11738050391723309 8 0.12829962056069663 9 0.12556984139983077 10-11 0.08735293314770835 12-13 0.05596047279775066 14-15 0.11874539349766604 16-17 0.06551469986078126 18-19 0.028662681189091804 20-21 0.03412223951082358 22-23 0.10100182895203778 24-25 0.18971965168017907 26-27 0.18835476209974614 28-29 0.1501378538476237 30-31 0.1624218600715202 32-33 0.20473343706494143 34-35 0.18153031419758142 36-37 0.18153031419758142 38-39 0.21428766412797204 40-41 0.16378674965195317 42-43 0.10782627685420249 44-45 0.09963693937160485 46-47 0.10100182895203778 48-49 0.13921873720416017 50-51 0.14467829552589195 52-53 0.16651652881281906 54-55 0.23749078699533208 56-57 0.1992738787432097 58-59 0.15150274342805667 60-61 0.23749078699533208 62-63 0.24704501405836266 64-65 0.26069390986269214 66-67 0.14877296426719078 68-69 0.14467829552589195 70-71 0.15559741216935552 72-73 0.1624218600715202 74-75 0.17197608713455081 76-77 0.15832719133022138 78-79 0.18971965168017907 80-81 0.26478857860399097 82-83 0.2989108181148145 84-85 0.22930144951273443 86-87 0.238855676575765 88-89 0.2306663390931674 90-91 0.19517921000191085 92-93 0.16924630797368492 94-95 0.16105697049108728 96-97 0.1460431851063249 98-99 0.1651516392323861 100-101 0.1869898725193132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 36633.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.32885649550951 #Duplication Level Percentage of deduplicated Percentage of total 1 67.00132100396301 27.69087980782355 2 13.626155878467635 11.263068817732645 3 6.55217965653897 8.123822782736877 4 3.7054161162483488 6.125624436983048 5 2.2589167767503304 4.667922365080665 6 1.5918097754293263 3.947260666612071 7 0.977542932628798 2.828051210657058 8 0.8454425363276089 2.7952938607266673 9 0.5680317040951123 2.1128490705101957 >10 2.7741083223249667 18.535200502279363 >50 0.046235138705416116 1.2038326099418557 >100 0.02642007926023778 2.014577020719024 >500 0.019815059445178335 5.664291758796713 >1k 0.006605019815059445 3.0273250894002675 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1109 3.0273250894002675 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 756 2.0637130456146098 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 706 1.9272240875713154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 613 1.673354625610788 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 359 0.9799907187508531 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 149 0.406737094969017 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 120 0.32757349930390633 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 110 0.3002757076952474 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.27024813692572275 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63 0.17197608713455081 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.16105697049108728 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.15832719133022138 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 55 0.1501378538476237 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.14740807468675785 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 53 0.14467829552589195 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 50 0.1364889580432943 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.13375917888242841 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 45 0.12284006223896486 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 45 0.12284006223896486 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.12011028307809898 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 42 0.11465072475636721 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 42 0.11465072475636721 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.10919116643463543 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.10646138727376955 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 39 0.10646138727376955 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.10646138727376955 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 38 0.10373160811290366 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 38 0.10373160811290366 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 38 0.10373160811290366 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.002729779160865886 0.0 16-17 0.0 0.0 0.0 0.008189337482597658 0.0 18-19 0.0 0.0 0.0 0.008189337482597658 0.0 20-21 0.0 0.0 0.0 0.010919116643463544 0.0 22-23 0.0 0.0 0.0 0.016378674965195315 0.0 24-25 0.0 0.0 0.0 0.02183823328692709 0.0 26-27 0.0 0.0 0.0 0.03548712909125652 0.0 28-29 0.0 0.0 0.0 0.06414981028034832 0.0 30-31 0.0 0.0 0.0 0.09281249146944012 0.0 32-33 0.0 0.0 0.0 0.11601561433680016 0.0 34-35 0.0 0.0 0.0 0.15696230174978842 0.0 36-37 0.0 0.0 0.0 0.22520678077143558 0.0 38-39 0.0 0.0 0.0 0.28116725356918626 0.0 40-41 0.0 0.0 0.0 0.3207490514017416 0.0 42-43 0.0 0.0 0.0 0.35760107007343106 0.0 44-45 0.0 0.0 0.0 0.38080419294079104 0.0 46-47 0.0 0.0 0.0 0.41492643245161465 0.0 48-49 0.0 0.0 0.0 0.45450823028417 0.0 50-51 0.0 0.0 0.0 0.47498157399066415 0.0 52-53 0.0 0.0 0.0 0.49954958643845715 0.0 54-55 0.0 0.0 0.0 0.5268473780471159 0.0 56-57 0.0 0.0 0.0 0.5596047279775066 0.0 58-59 0.0 0.0 0.0 0.5855376300057326 0.0 60-61 0.0 0.0 0.0 0.6142003111948244 0.0 62-63 0.0 0.0 0.0 0.6360385444817515 0.0 64-65 0.0 0.0 0.0 0.6647012256708432 0.0 66-67 0.0 0.0 0.0 0.6988234651816668 0.0 68-69 0.0 0.0 0.0 0.7343105942729233 0.0 70-71 0.0 0.0 0.0 0.7725275025250458 0.0 72-73 0.0 0.0 0.0 0.8189337482597658 0.0 74-75 0.0 0.0 0.0 0.8598804356727541 0.0 76-77 0.0 0.0 0.0 0.9226653563726694 0.0 78-79 0.0 0.0 0.0 0.9718013812682553 0.0 80-81 0.0 0.0 0.0 1.0304916332268719 0.0 82-83 0.0 0.0 0.0 1.0823574372833238 0.0 84-85 0.0 0.0 0.0 1.1410476892419403 0.0 86-87 0.0 0.0 0.0 1.195643272459258 0.0 88-89 0.0 0.0 0.0 1.24750907651571 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 280 0.0 35.625 1 ATGGGAG 55 0.0025471277 34.545456 5 ATATATC 55 0.0025471277 34.545456 3 GAGGCTG 55 0.0025471277 34.545456 7 GTATCAA 435 0.0 33.850574 1 GGAGGCT 60 0.0039013992 31.666668 6 TTCGGGG 35 0.008341015 27.142859 94-95 GTACATG 415 0.0 26.3253 1 AACGCAG 555 0.0 25.675676 6 AGTACTT 435 0.0 25.66092 12-13 TCAACGC 585 0.0 25.17094 4 GGAAGCA 95 0.001203911 25.0 8 GGGAAGC 95 0.001203911 25.0 7 CAACGCA 570 0.0 24.999998 5 ATCAACG 595 0.0 24.7479 3 TACATGG 430 0.0 24.302326 2 TATCAAC 610 0.0 24.139345 2 GAAGCAG 100 0.0016232299 23.75 9 CATGGGG 160 1.778697E-6 23.749998 4 CGCAGAG 610 0.0 23.360657 8 >>END_MODULE