FastQCFastQC Report
Fri 10 Feb 2017
SRR2031435_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031435_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924823
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19160.2071747783089305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15430.16684273639388295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.052.6018751
GTATCAA20150.045.7083441
TCAACGC24050.037.115784
CAACGCA24550.036.359865
ATCAACG24800.036.184783
AACGCAG25800.034.7690936
TATCAAC26400.034.1716162
ACGCAGA29400.030.5116527
GTACATG30800.029.9033471
TACATGG30500.029.8894022
CGCAGAG30450.029.1477858
CCCTACG1004.969572E-528.485251
ACATGGG31650.027.6032333
CATGGGG21250.024.3547344
GCAGAGT36600.023.4719289
AGAGTAC30600.022.02511210-11
TCTACGC1805.185284E-621.102473
GAGTACT19350.020.23598112-13
CAGAGTA34200.019.84545910-11
GAGTACA21500.018.769351
Produced by FastQC (version 0.11.5)