FastQCFastQC Report
Fri 10 Feb 2017
SRR2031435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924823
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.2517238433732725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18600.20111956558173832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.11731974658934737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.059.145921
GTATCAA23850.046.0610281
TCAACGC28950.037.241184
ATCAACG29100.037.0492133
CAACGCA29800.036.178935
AACGCAG30750.035.2156646
TATCAAC30800.035.1584972
ACGCAGA35900.030.0315367
CGCAGAG36950.029.1781378
TACATGG31900.025.012942
GCAGAGT43050.024.9333979
GTACATG32450.024.9140471
ACATGGG34000.022.2108083
GAGTACT24650.022.0620812-13
ACCGTAT1302.9430806E-421.9206818
AGAGTAC37650.021.50827410-11
CAGAGTA41150.021.17933810-11
CAGTGCG1353.7979713E-421.1088079
GTATATA5000.020.9248681
CTAATAC3950.020.4408053
Produced by FastQC (version 0.11.5)