FastQCFastQC Report
Fri 10 Feb 2017
SRR2031434_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031434_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202275
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.21933417895240276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17870.14863487970722172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14350.057.8782771
GTATCAA27050.048.2497251
TCAACGC33700.037.6051184
ATCAACG34050.037.218573
CAACGCA34450.036.648655
TATCAAC35250.036.086212
AACGCAG35950.035.1136476
ACGCAGA40600.030.9751287
CGCAGAG41950.030.317698
GCAGAGT49250.025.3410539
GTACATG37000.025.141071
TACATGG37400.024.3666152
ACATGGG37550.023.6372643
AGAGTAC43900.021.673310-11
GTATAAC3550.021.3905051
CAGAGTA46850.020.76440210-11
GTATTAC3800.019.9832341
CATGGGG28050.019.7978734
GAGTACT28600.019.49606512-13
CGATACT1250.00605766818.9856534
Produced by FastQC (version 0.11.5)