FastQCFastQC Report
Fri 10 Feb 2017
SRR2031434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202275
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30420.25302031565157723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.17575014035890293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13580.11295252749994801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.052.2489971
GTATCAA27150.051.3117871
TCAACGC33800.040.467584
CAACGCA34000.040.2295345
ATCAACG34050.040.1704643
TATCAAC35800.038.4721452
AACGCAG36200.038.1782346
ACGCAGA41750.032.989297
CGCAGAG43450.031.5892648
GCAGAGT50000.026.7861639
ACATGGG37450.026.3780673
TACATGG39000.026.182152
GTACATG39300.026.0114961
TAGGCCG1000.001655068523.74665
CATGGGG27150.023.2655454
AGAGTAC43800.023.04179410-11
CAGAGTA47400.021.09138910-11
GAGTACT30650.020.99879612-13
AGTACTT32800.020.34642412-13
GTACTTT33500.019.4282514-15
Produced by FastQC (version 0.11.5)