FastQCFastQC Report
Fri 10 Feb 2017
SRR2031433_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031433_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644084
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24660.14999233615800653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.10315774619788283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.057.7034261
GTATCAA31650.042.5838661
TCAACGC37800.034.913954
ATCAACG38750.034.0579953
CAACGCA39850.033.1178785
TATCAAC40800.032.808172
AACGCAG41800.031.7855196
GTACATG52750.029.2389241
ACGCAGA46350.028.3581317
TACATGG54150.027.875442
ACATGGG53650.027.1653123
CGCAGAG48600.027.0452568
GCAGAGT56400.022.967729
CATGGGG38550.022.9052264
AGAGTAC48600.020.20763610-11
CAGAGTA53950.020.09445410-11
GAGTACT30200.019.00902712-13
CATGGGA18600.018.631854
CGTATAC1801.2391314E-418.4616953
ATACCGC1559.6935447E-418.3682236
Produced by FastQC (version 0.11.5)