FastQCFastQC Report
Fri 10 Feb 2017
SRR2031433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644084
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.1954887949764124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.13989552845231754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19100.051.2795031
GTATCAA36150.045.6384771
TCAACGC43300.037.2939574
ATCAACG43700.036.9525953
CAACGCA44000.036.9165275
TATCAAC46250.035.3259772
AACGCAG46150.035.1966936
ACGCAGA51100.031.6942847
CGCAGAG52950.030.3178338
CGATAGC908.954749E-426.3860844
TACATGG50850.025.685572
GCAGAGT62950.024.9735169
ACATGGG52200.024.9302983
GTACATG53100.024.7129481
TACCGTA1551.4125599E-624.5135237
GTACCGT2151.6661943E-924.2997446
GTATAGG4500.022.187931
AGAGTAC53600.022.06388710-11
CATGGGG38500.021.5886144
CAGAGTA59150.020.55571710-11
Produced by FastQC (version 0.11.5)