FastQCFastQC Report
Fri 10 Feb 2017
SRR2031432_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031432_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008057
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22850.2266736900790332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15140.15018991981604216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.061.9601361
GTATCAA22850.050.277811
TCAACGC27500.040.5719154
CAACGCA28100.039.874575
ATCAACG28000.039.8474163
TATCAAC29400.038.1095162
AACGCAG29450.038.02976
ACGCAGA33250.033.6834537
CGCAGAG34300.032.6523258
ACACCGT2151.8189894E-1228.6946626
GCAGAGT40500.027.5365229
GTACATG33900.025.6270481
TACATGG34000.024.1566092
ACATGGG33550.023.4912383
CAGAGTA38000.023.04026410-11
AGAGTAC35750.022.43289410-11
CATGGGG25400.022.4304434
GAGTACT23550.021.76243812-13
CCGATCG550.002882195921.5856318-19
GTATATA6000.021.3628731
Produced by FastQC (version 0.11.5)