FastQCFastQC Report
Fri 10 Feb 2017
SRR2031432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008057
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27770.2754804539822649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19020.18867980679663948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11900.11804888017245058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.058.8610341
GTATCAA27450.051.7848431
TCAACGC31950.043.856814
CAACGCA32550.043.0483935
ATCAACG32550.043.0483933
AACGCAG33650.041.63916
TATCAAC34300.040.9905242
ACGCAGA38000.036.8725177
CGCAGAG38800.035.9898458
GCAGAGT44550.031.2380779
GCGTTAT700.00835532927.166631
GTACATG32900.024.565571
TACATGG32600.024.4780922
GAGTACT26200.024.20281612-13
GTATAAG2950.024.1736971
AGAGTAC40400.023.92475110-11
ACATGGG32350.023.6394563
GTATATA6450.023.5865331
CAGAGTA42950.023.2784110-11
CATGGGG23450.022.0784974
Produced by FastQC (version 0.11.5)