FastQCFastQC Report
Fri 10 Feb 2017
SRR2031431_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031431_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903936
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.15875017700368166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.11483113848768055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10300.047.0047571
GTATCAA19900.041.502581
TCAACGC19850.040.6551364
CAACGCA20150.039.814265
ATCAACG20800.038.570073
AACGCAG21050.038.5437976
TATCAAC22850.035.5232622
ACGCAGA23950.034.0748067
CGCAGAG25950.031.4486188
GCAGAGT30800.026.1869329
GTACATG26300.022.9206451
GTATAAG3350.022.6701341
GCACCGT1050.002210100922.5938996
AGAGTAC26600.021.76028810-11
TACATGG26700.021.5118142
CAGAGTA29100.021.19516410-11
ACATGGG26950.020.785013
GAGTACT17000.020.65233812-13
CATGGGG19100.019.8830784
GTATATA6050.018.8293251
Produced by FastQC (version 0.11.5)