FastQCFastQC Report
Fri 10 Feb 2017
SRR2031431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903936
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17450.19304464032851884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12560.13894789011611441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9300.048.5761031
GTATCAA16000.042.79811
ACCGTAC500.001616373237.996468
CAACGCA19250.035.0356945
TCAACGC19500.034.586524
ATCAACG19750.034.38923
AACGCAG19950.033.5683026
TATCAAC21050.032.2654042
ACGCAGA23450.028.5581077
GTACATG25650.028.3652131
TACATGG25900.028.2406122
CGCAGAG24350.027.6976248
CCGTACA700.00839445327.1403279
ACATGGG25650.026.6641833
TACCGTA1109.480436E-525.9066777
CTAACCG500.001653310423.75172866-67
CATGGGG15050.023.3533044
GCAGAGT29400.022.132299
GTATATA5200.021.0332551
AGAGTAC26500.020.70090110-11
Produced by FastQC (version 0.11.5)