FastQCFastQC Report
Fri 10 Feb 2017
SRR2031430_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031430_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631574
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35150.5565460262772058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.4279783524970946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15490.24526025453866057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.063.5092851
GTATCAA28050.047.5593871
CCACGCG250.001617894237.99034540-41
ATCAACG35600.037.206353
CAACGCA36100.037.0855525
TCAACGC35800.036.9984864
TATCAAC36350.036.6998862
AACGCAG36850.036.453816
CGCAGAG41150.032.759898
ACGCAGA41800.032.250477
CATGGGG13600.029.3226494
GCAGAGT45800.029.021579
GTACATG33800.028.9343221
ACATGGG32950.028.3843
TACATGG34350.028.3328272
GAGTACT28000.025.76997212-13
CAGAGTA45750.024.43589210-11
ATGGGAT7600.023.7373815
AGAGTAC42550.023.48447810-11
GTACTTT30900.023.19780314-15
Produced by FastQC (version 0.11.5)