##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031430_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 631574 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.956692327423234 34.0 33.0 34.0 31.0 34.0 2 33.025197364046015 34.0 33.0 34.0 31.0 34.0 3 33.18124400307802 34.0 34.0 34.0 31.0 34.0 4 36.14089560368223 37.0 37.0 37.0 35.0 37.0 5 36.14760107287507 37.0 37.0 37.0 35.0 37.0 6 36.23575384673847 37.0 37.0 37.0 35.0 37.0 7 36.2663567531279 37.0 37.0 37.0 35.0 37.0 8 36.34645979726841 37.0 37.0 37.0 35.0 37.0 9 38.2995294296472 39.0 39.0 39.0 37.0 39.0 10-11 38.385602478886085 39.0 39.0 39.0 37.5 39.0 12-13 38.43740638468335 39.0 39.0 39.0 38.0 39.0 14-15 40.05705665527714 41.0 40.0 41.0 38.5 41.0 16-17 40.05897092027221 41.0 40.0 41.0 38.5 41.0 18-19 40.105482492946194 41.0 40.5 41.0 39.0 41.0 20-21 40.055912687982726 41.0 40.0 41.0 39.0 41.0 22-23 39.98812728199704 41.0 40.0 41.0 38.5 41.0 24-25 39.88385684021192 41.0 40.0 41.0 38.0 41.0 26-27 39.794336055632435 41.0 40.0 41.0 38.0 41.0 28-29 39.71761978802167 41.0 40.0 41.0 38.0 41.0 30-31 39.53516135876397 41.0 40.0 41.0 37.0 41.0 32-33 39.32463258462191 41.0 40.0 41.0 35.5 41.0 34-35 39.22967063241995 41.0 40.0 41.0 35.0 41.0 36-37 39.10926668925573 41.0 40.0 41.0 35.0 41.0 38-39 38.93299914182661 41.0 39.0 41.0 35.0 41.0 40-41 38.8318803497294 41.0 39.0 41.0 35.0 41.0 42-43 38.958017587804434 41.0 40.0 41.0 35.0 41.0 44-45 38.88030222903413 41.0 40.0 41.0 35.0 41.0 46-47 38.63352592095305 41.0 40.0 41.0 35.0 41.0 48-49 38.39827399481295 41.0 39.0 41.0 35.0 41.0 50-51 37.39328803908964 40.0 38.0 40.5 34.0 40.5 52-53 37.450770614369816 39.5 38.0 40.5 33.5 41.0 54-55 37.88322350191743 40.0 39.0 41.0 34.0 41.0 56-57 37.898965916899684 41.0 39.0 41.0 34.0 41.0 58-59 37.92767514178861 41.0 39.0 41.0 34.0 41.0 60-61 37.76052054074424 41.0 38.0 41.0 34.0 41.0 62-63 37.54239724877845 40.5 37.0 41.0 34.0 41.0 64-65 37.29552356493459 40.0 37.0 41.0 33.5 41.0 66-67 37.053386459860604 39.5 36.0 41.0 33.0 41.0 68-69 36.74591971803779 39.0 36.0 41.0 33.0 41.0 70-71 36.382495162878776 39.0 35.0 41.0 33.0 41.0 72-73 36.011714383429336 37.0 35.0 40.5 32.0 41.0 74-75 35.61246504764287 37.0 35.0 39.0 32.0 41.0 76-77 35.21875029687733 36.5 35.0 39.0 32.0 41.0 78-79 34.813326704392516 36.0 35.0 38.5 31.0 40.0 80-81 34.4026859877069 35.5 35.0 37.0 31.0 39.0 82-83 34.07146510147663 35.0 35.0 37.0 31.0 39.0 84-85 33.7509927577766 35.0 35.0 36.5 30.0 38.0 86-87 33.501426594508324 35.0 35.0 36.0 30.0 37.0 88-89 33.29654244791584 35.0 35.0 36.0 30.0 37.0 90-91 33.0843749109368 35.0 35.0 35.5 29.0 36.5 92-93 32.99723547834458 35.0 35.0 35.0 29.0 36.0 94-95 32.893864376937614 35.0 35.0 35.0 29.0 36.0 96-97 32.81071735061924 35.0 34.5 35.0 29.0 36.0 98-99 32.733286202408586 35.0 34.0 35.0 29.0 36.0 100-101 32.43126062820826 35.0 34.0 35.0 27.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17386085086670278 1101 2 0.09607855620063788 1101 3 0.11648407412938866 1101 4 0.25248534130154354 1101 5 0.11089251979422698 1101 6 0.17228643546582845 1101 7 0.16176168666984125 1101 8 0.09869478379135899 1101 9 0.1340811149973078 1101 10-11 0.12760982002449595 1101 12-13 0.050221042990422404 1101 14-15 0.09127546743310688 1101 16-17 0.13102371194332108 1101 18-19 0.12561641117660116 1101 20-21 0.051868727608159304 1101 22-23 0.07388594532194048 1101 24-25 0.03787970295032039 1101 26-27 -0.06253137117752061 1101 28-29 0.08040158889833293 1101 30-31 0.21736895184116634 1101 32-33 0.06391424208851504 1101 34-35 0.09701952299622008 1101 36-37 0.08800457437370568 1101 38-39 0.10016498731072687 1101 40-41 0.11615198225742773 1101 42-43 0.22428500811006558 1101 44-45 0.3522638687419928 1101 46-47 0.40907123683059154 1101 48-49 0.4858636585238969 1101 50-51 0.34837307296920983 1101 52-53 0.4350169242355193 1101 54-55 0.6198116957955264 1101 56-57 0.6052467090123201 1101 58-59 0.45261119575337716 1101 60-61 0.4234043296685144 1101 62-63 0.3571649461915385 1101 64-65 0.298112169656612 1101 66-67 0.26014512360250563 1101 68-69 0.32686555816736274 1101 70-71 0.35642751680828866 1101 72-73 0.26957975575611925 1101 74-75 0.2949885606564706 1101 76-77 0.1297500276973267 1101 78-79 0.197655710518724 1101 80-81 0.19649268446119805 1101 82-83 0.11055959822127903 1101 84-85 0.2502351344062106 1101 86-87 0.363217471534206 1101 88-89 0.3179944973281863 1101 90-91 0.3755979456628751 1101 92-93 0.14032617215642063 1101 94-95 0.19297597540165867 1101 96-97 0.2552815289615822 1101 98-99 0.291580882377275 1101 100-101 0.20839965917319603 1102 1 0.13334519709137993 1102 2 0.07950397056527692 1102 3 0.0999094884940277 1102 4 -0.04217395888262132 1102 5 0.13299196730804397 1102 6 0.12624591981205668 1102 7 0.20780220232361302 1102 8 0.17051798821124464 1102 9 0.11198166748349792 1102 10-11 0.13865954378140088 1102 12-13 0.15243098774180197 1102 14-15 0.27175428879590413 1102 16-17 0.2231047432508646 1102 18-19 0.17349854745653204 1102 20-21 0.12645436296727297 1102 22-23 0.14478833942875013 1102 24-25 0.13272316519709193 1102 26-27 0.2763268240342711 1102 28-29 0.2599595999480542 1102 30-31 0.3407575337932798 1102 32-33 0.307928975053521 1102 34-35 0.3272221012651002 1102 36-37 0.24454232759654815 1102 38-39 0.16738414016523961 1102 40-41 0.0240709509498771 1102 42-43 0.12852073555020382 1102 44-45 0.22611285585065133 1102 46-47 0.16413569355250246 1102 48-49 0.2354032533673589 1102 50-51 0.23971745602629824 1102 52-53 0.19560624283588623 1102 54-55 0.23491298492996293 1102 56-57 0.3455782007250221 1102 58-59 0.28594452908670576 1102 60-61 0.21714281953960324 1102 62-63 0.4243840990460441 1102 64-65 0.2916664974650871 1102 66-67 0.32460184551779037 1102 68-69 0.17032780494452737 1102 70-71 0.34998184461676374 1102 72-73 -0.0232379238018936 1102 74-75 0.07859813708373764 1102 76-77 0.06989735734742197 1102 78-79 0.24645865711173087 1102 80-81 0.15689784099895832 1102 82-83 0.08477690945516514 1102 84-85 0.12776736276716605 1102 86-87 0.2656115783482065 1102 88-89 0.41191714926189604 1102 90-91 0.36362741159289413 1102 92-93 0.19557479094095243 1102 94-95 0.3347807636152922 1102 96-97 0.33262959525992386 1102 98-99 0.44903944591318634 1102 100-101 0.2590442263923478 1103 1 0.18802194420779017 1103 2 0.2911158732501704 1103 3 0.28411032206967946 1103 4 0.04022402502743461 1103 5 0.05596376143424209 1103 6 0.11029166954550362 1103 7 0.28617769907654633 1103 8 0.2924138881821676 1103 9 0.3305141866266581 1103 10-11 0.27165363120990804 1103 12-13 0.26482800237783977 1103 14-15 0.26579325361125683 1103 16-17 0.31095004711827556 1103 18-19 0.2951133579634657 1103 20-21 0.3832635129139632 1103 22-23 0.2504004359684373 1103 24-25 0.30155131291445514 1103 26-27 0.2360001786916328 1103 28-29 0.27951954954581026 1103 30-31 0.2889740696079599 1103 32-33 0.32165874145849216 1103 34-35 0.3012358649560909 1103 36-37 0.25187973188074153 1103 38-39 0.3270720339328079 1103 40-41 0.3378588737956676 1103 42-43 0.45767649788421494 1103 44-45 0.3045755872648215 1103 46-47 0.11598700691879316 1103 48-49 0.021159771735106858 1103 50-51 0.18564650516849213 1103 52-53 -0.008726412005636064 1103 54-55 -0.06359433308883666 1103 56-57 0.03026367130755858 1103 58-59 0.06452357338252313 1103 60-61 -0.17916424646796258 1103 62-63 -0.0037684646837234936 1103 64-65 -0.09185099793086238 1103 66-67 0.005061281400308815 1103 68-69 -0.011178446872854408 1103 70-71 0.011937839576539488 1103 72-73 -0.11323016960978549 1103 74-75 -0.0936176233117294 1103 76-77 -0.07742744637459253 1103 78-79 0.007886397734004902 1103 80-81 0.004683049877506562 1103 82-83 0.13443281718027578 1103 84-85 0.03471705744584597 1103 86-87 -0.18676798935558026 1103 88-89 -0.19596304847000567 1103 90-91 -0.08251972420239184 1103 92-93 -0.1427454571932003 1103 94-95 -0.27146620740341376 1103 96-97 -0.1611815901621938 1103 98-99 -0.10235146229656777 1103 100-101 -0.041818427479903164 1104 1 0.1375878384656204 1104 2 0.24437615706550986 1104 3 0.19931300454378942 1104 4 0.31477045937268144 1104 5 0.1575804310947433 1104 6 0.2080283320208025 1104 7 0.30469156052197377 1104 8 0.23515384387344085 1104 9 0.18328946954385117 1104 10-11 0.14480194568209726 1104 12-13 0.027563766703330828 1104 14-15 0.11225454335438201 1104 16-17 0.05258827237424413 1104 18-19 0.10945980428404312 1104 20-21 0.06937512299719373 1104 22-23 0.08190453751924309 1104 24-25 0.15060825561249658 1104 26-27 0.04004784050828647 1104 28-29 0.11275408639988882 1104 30-31 0.06644248467292613 1104 32-33 -0.04607073581831855 1104 34-35 -0.05022213157106137 1104 36-37 0.0646244777043421 1104 38-39 -0.012368718117969024 1104 40-41 -0.0620153852307368 1104 42-43 -0.0444910369939322 1104 44-45 -0.14643034052392778 1104 46-47 -0.24685054771865822 1104 48-49 -0.23379202755835138 1104 50-51 -0.3105782717909733 1104 52-53 -0.21942936392468226 1104 54-55 -0.016630079451338986 1104 56-57 0.120141522933217 1104 58-59 -0.10395735590314814 1104 60-61 -0.07624525958094353 1104 62-63 0.10463821755087821 1104 64-65 0.12999200548210865 1104 66-67 0.17487746007224558 1104 68-69 -0.008076836190859638 1104 70-71 -0.1541869433705898 1104 72-73 -0.05355709614112669 1104 74-75 -0.11442476060889817 1104 76-77 0.04123910053876756 1104 78-79 0.0033537350191963355 1104 80-81 -0.06170818418033974 1104 82-83 -0.09914002276942568 1104 84-85 0.22148731072410044 1104 86-87 0.1998914921125916 1104 88-89 0.1464865054489195 1104 90-91 0.17098006622931194 1104 92-93 0.08327993050814086 1104 94-95 0.1432365050090212 1104 96-97 -0.1544334912459604 1104 98-99 -0.1946902444054217 1104 100-101 0.02859651773295724 1105 1 -0.13422880461548914 1105 2 0.04370466229595138 1105 3 -0.05838005020245873 1105 4 -0.08622013415099872 1105 5 -0.06714278542069962 1105 6 -0.010204693312864777 1105 7 -0.004451312476724922 1105 8 0.03439078163530951 1105 9 0.09328697224925264 1105 10-11 -0.007978375636476187 1105 12-13 0.04865341957937375 1105 14-15 -0.08769412336777549 1105 16-17 -0.05246458702806933 1105 18-19 -0.033513086195718245 1105 20-21 0.05331058180571091 1105 22-23 0.03670766746180476 1105 24-25 -0.026433282720972784 1105 26-27 0.06310758134460315 1105 28-29 0.09475275450943599 1105 30-31 0.18946615347616103 1105 32-33 0.15432883131727948 1105 34-35 0.08061113239298123 1105 36-37 0.2315117387271286 1105 38-39 0.164769937227625 1105 40-41 0.22671210443323275 1105 42-43 0.3065425654922649 1105 44-45 0.2400912055788993 1105 46-47 0.15678717807839604 1105 48-49 0.4061619112301784 1105 50-51 0.22884291081675912 1105 52-53 0.217418217861713 1105 54-55 0.3017458466287408 1105 56-57 0.10013958059297323 1105 58-59 0.08964124561187958 1105 60-61 0.09131072143961205 1105 62-63 0.11713440223968519 1105 64-65 0.128880709386209 1105 66-67 0.03321703664207831 1105 68-69 -0.03193154914072238 1105 70-71 0.2391522169834701 1105 72-73 0.10258069902521783 1105 74-75 0.07084536342339476 1105 76-77 0.07813524083123013 1105 78-79 0.0934020160480813 1105 80-81 0.14708784234648675 1105 82-83 0.09801861093294661 1105 84-85 0.17502514473724773 1105 86-87 0.1291070641317944 1105 88-89 0.009514058932637681 1105 90-91 -0.027663070372703658 1105 92-93 0.15804615598609217 1105 94-95 0.07408364283192981 1105 96-97 -0.10519100394053993 1105 98-99 -0.14924919443178908 1105 100-101 -0.18400927044095994 1106 1 0.058627598470835096 1106 2 0.037076315757708755 1106 3 0.08525570144168171 1106 4 -0.023939568670186873 1106 5 0.10928256800480796 1106 6 -0.08199797175520018 1106 7 0.06138163247693029 1106 8 0.024484510598078657 1106 9 0.040319751494244827 1106 10-11 0.0013077825117377984 1106 12-13 -0.05256220881894791 1106 14-15 -0.1551336655975959 1106 16-17 0.03117297705457389 1106 18-19 0.07357743216169865 1106 20-21 0.10386078635233531 1106 22-23 0.08023337819662402 1106 24-25 0.10009304942133213 1106 26-27 -0.02674146841977887 1106 28-29 -0.010789423523810626 1106 30-31 -0.02008280825549491 1106 32-33 0.020166954666670733 1106 34-35 0.12454852558856544 1106 36-37 0.1920238820651221 1106 38-39 -0.040455064066968305 1106 40-41 0.12439088854591773 1106 42-43 0.1903924437999578 1106 44-45 0.19273348200166396 1106 46-47 -0.1389018074738786 1106 48-49 0.03446572770783263 1106 50-51 -0.028195823583324398 1106 52-53 0.04637192300386772 1106 54-55 0.21160680670331544 1106 56-57 0.13871992340276762 1106 58-59 0.3158840605877984 1106 60-61 0.03749683298225648 1106 62-63 0.01320270527940437 1106 64-65 0.2544865816869759 1106 66-67 0.33007245583321065 1106 68-69 0.31977473225330044 1106 70-71 0.09103326923347765 1106 72-73 0.056372579300706604 1106 74-75 -0.12996246631094976 1106 76-77 -0.005349560577300849 1106 78-79 0.24275342198252048 1106 80-81 0.1511295802160859 1106 82-83 0.18850589797142447 1106 84-85 0.21255815701005076 1106 86-87 0.13068647483036244 1106 88-89 0.10481078100814045 1106 90-91 0.28192602392588384 1106 92-93 0.31198691513890253 1106 94-95 0.31451267291841134 1106 96-97 0.08556944347284201 1106 98-99 0.3029619805462076 1106 100-101 0.42247925941249065 1107 1 0.1937271521094317 1107 2 0.17317140578228418 1107 3 0.23332982752624076 1107 4 0.2580856081190177 1107 5 0.3173396420807393 1107 6 0.18074338939631218 1107 7 0.18511401624742518 1107 8 0.18709547403656757 1107 9 0.28368451401994577 1107 10-11 0.1732008062340853 1107 12-13 0.04692497709810084 1107 14-15 0.27329428966595515 1107 16-17 0.2133122518426802 1107 18-19 0.11937085209248721 1107 20-21 0.2514220983539417 1107 22-23 0.22485823400683813 1107 24-25 0.21095868962394349 1107 26-27 0.35511193445900346 1107 28-29 0.3395002098580093 1107 30-31 0.28196343277966207 1107 32-33 0.2773363212347064 1107 34-35 0.37938201773962277 1107 36-37 0.41358715003241997 1107 38-39 0.28698362393142673 1107 40-41 0.25831059364647047 1107 42-43 0.40250313139713256 1107 44-45 0.392930332395359 1107 46-47 0.29581156832229993 1107 48-49 0.3178513037146544 1107 50-51 0.24423885217775165 1107 52-53 0.12609884010101524 1107 54-55 0.2816190941801153 1107 56-57 0.28252227056160706 1107 58-59 0.18898827845228539 1107 60-61 0.2186393175602177 1107 62-63 0.1710554060166558 1107 64-65 0.20696789970693175 1107 66-67 0.20026780163791358 1107 68-69 0.23765426482765406 1107 70-71 0.34056057752376745 1107 72-73 0.35910716980173873 1107 74-75 0.22237215428186374 1107 76-77 0.23118562172873425 1107 78-79 0.0480784132057579 1107 80-81 0.19987575605239272 1107 82-83 0.24823306583340354 1107 84-85 0.2756204971474716 1107 86-87 0.15022607059744786 1107 88-89 0.21204910790518738 1107 90-91 0.3429534074456271 1107 92-93 0.29257019413985574 1107 94-95 0.38508165832389096 1107 96-97 0.41460926492828776 1107 98-99 0.32189221776974364 1107 100-101 0.2946092559017828 1108 1 0.26096541399336814 1108 2 0.15131080502404615 1108 3 0.0671122713874226 1108 4 0.24104273847772362 1108 5 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52-53 -0.7986386927073923 2316 54-55 -0.718396687075078 2316 56-57 -0.7049906036202671 2316 58-59 -0.5427993224646883 2316 60-61 -0.6045084614357563 2316 62-63 -0.7741437700357068 2316 64-65 -0.6983910823190485 2316 66-67 -0.6749464911957332 2316 68-69 -0.7098182425646158 2316 70-71 -1.4852251628662785 2316 72-73 -0.9714802251283245 2316 74-75 -0.6523562420103772 2316 76-77 -0.5209539842382398 2316 78-79 -0.54868144592578 2316 80-81 -0.6700393574065302 2316 82-83 -0.6767652729818394 2316 84-85 -0.5442580702467978 2316 86-87 -0.7110184890224716 2316 88-89 -0.6401669125606162 2316 90-91 -0.6153367350840284 2316 92-93 -0.6579464343195554 2316 94-95 -0.4620669180407688 2316 96-97 -0.6484733956252171 2316 98-99 -0.7306992304511297 2316 100-101 -0.8341790759364862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 214.0 3 77.0 4 68.0 5 76.0 6 68.0 7 199.0 8 433.0 9 367.0 10 266.0 11 305.0 12 341.0 13 367.0 14 400.0 15 498.0 16 615.0 17 973.0 18 1339.0 19 2070.0 20 2831.0 21 4170.0 22 5747.0 23 5802.0 24 4427.0 25 3383.0 26 2684.0 27 2484.0 28 2578.0 29 2772.0 30 3305.0 31 3908.0 32 4845.0 33 6661.0 34 10245.0 35 18419.0 36 38073.0 37 108964.0 38 267292.0 39 124102.0 40 206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4429423673397 20.41839201361057 14.108928927850664 22.029736691199066 2 14.713962943362983 22.304489322529637 41.58461962601126 21.396928108096116 3 16.99998891125998 28.736152935902332 32.45246494770075 21.811393205136937 4 11.324291053466268 18.950922315715093 41.89989274109094 27.824893889727704 5 11.448282638739208 39.91137426937657 36.254453474661155 12.38588961722306 6 26.599518651082242 40.01406971161845 20.406944226585736 12.979467410713577 7 24.093120552764617 35.45173610560847 24.309915849194148 16.145227492432767 8 24.426558973448635 34.964528440646156 22.74284862932062 17.866063956584586 9 26.783891562216684 15.483322872485433 22.036093095206397 35.696692470091484 10-11 22.457240769068072 27.4985958827877 31.20863399397477 18.835529354169456 12-13 24.17762584708577 26.262668664717193 30.0698420040489 19.48986348414813 14-15 21.854152452992857 27.040568499408472 28.83384878949024 22.27143025810843 16-17 20.03111284664329 29.85573872812774 29.25051148474338 20.86263694048559 18-19 19.566217584904642 28.620865236668703 32.29080795914472 19.522109219281926 20-21 21.624057387500255 27.706669918920674 31.804272384720534 18.865000308858537 22-23 21.167103079184113 27.908983231459732 31.14773190934768 19.77618178000848 24-25 20.41411249712532 28.047913973719478 31.723856273939145 19.814117255216058 26-27 20.247155601128323 28.222416250127875 31.659723692631246 19.870704456112552 28-29 20.313867363374637 27.986797214859156 31.826512105723744 19.872823316042464 30-31 20.966621739475144 27.860302862126378 31.464837865694122 19.708237532704352 32-33 20.32923841109508 28.108929638040813 31.43605726175112 20.125774689112987 34-35 20.737003043553422 28.279115577315615 31.163195683514854 19.82068569561611 36-37 20.85263633696264 28.005122796444176 30.90997042100505 20.232270445588128 38-39 20.47931181922245 28.23951153380258 31.266961182739315 20.01421546423565 40-41 20.639977166325085 27.989645562695483 31.421832323128214 19.948544947851214 42-43 20.647947137401555 28.154800576798138 30.863454133455875 20.333798152344432 44-45 21.206472440632986 28.155988195916105 30.551266562357647 20.086272801093262 46-47 20.8909188863712 28.132199844713114 30.227225910725885 20.7496553581898 48-49 20.916374923018513 28.355897396064094 30.245143924664745 20.48258375625264 50-51 21.077422234130342 28.665227651601928 29.45927542447113 20.7980746897966 52-53 21.052281041719855 28.81047111452226 29.581152247582104 20.556095596175773 54-55 21.289689356424844 28.53634675030687 29.527603725723406 20.646360167544884 56-57 21.26598140921925 28.889787760540592 29.104247961676343 20.739982868563814 58-59 21.036162878823912 28.746539570193214 29.461695063595805 20.75560248738707 60-61 21.49101542197165 28.670742690731345 29.30047650599302 20.537765381303984 62-63 21.012098329278338 28.736983940313003 29.572265417315386 20.67865231309327 64-65 21.080117940720104 28.400696850284334 29.497720156672262 21.021465052323297 66-67 21.085007328559662 28.689190592491652 29.21840059040684 21.007401488541845 68-69 21.37111828937199 28.720794276768196 29.160103842571488 20.74798359128832 70-71 21.20552931971166 28.260430102241834 29.531060605460034 21.002979972586473 72-73 21.25397829716988 28.29019249645977 29.36566081359448 21.090168392775873 74-75 21.20750067199665 28.518110607080583 28.94477892301337 21.329609797909395 76-77 21.058903184273035 28.485940170804646 29.286606771931716 21.168549872990603 78-79 21.191996256671768 28.637571277886604 28.96138503144554 21.20904743399608 80-81 21.17106175991059 28.647324128799966 28.858942669314676 21.322671441974766 82-83 21.285230793903082 28.76720702897061 28.92233724228656 21.025224934839752 84-85 21.439486504070203 28.464034656214793 28.755930751043334 21.34054808867167 86-87 20.98934310658242 28.821695597356527 29.05457462483466 21.134386671226387 88-89 21.364217381679516 28.735796344916835 28.686999485054276 21.212986788349372 90-91 21.466479715894042 28.62955989905927 28.935839827176274 20.96812055787041 92-93 21.45771138361264 28.742303681942342 28.80962292501715 20.990362009427866 94-95 21.287240182926443 28.752878046924057 28.821221750740524 21.138660019408977 96-97 21.234602741007382 28.655013408566106 28.933648406231594 21.176735444194918 98-99 21.183514612970157 28.68182391178702 28.808993182490283 21.325668292752542 100-101 21.264199183497386 28.74040038446044 28.87513600446634 21.120264427575837 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 618.0 1 391.0 2 196.0 3 218.5 4 185.5 5 171.0 6 222.0 7 364.0 8 610.5 9 744.0 10 614.5 11 539.5 12 615.5 13 656.0 14 694.0 15 741.5 16 824.5 17 918.5 18 982.5 19 1097.5 20 1175.0 21 1262.5 22 1457.0 23 1760.0 24 2263.0 25 2969.0 26 4083.0 27 5581.5 28 7138.5 29 9275.0 30 11953.0 31 14395.5 32 17018.5 33 19580.5 34 21756.0 35 23295.0 36 24905.0 37 26357.0 38 25645.5 39 23977.5 40 22796.0 41 22757.5 42 22699.0 43 22456.0 44 22449.5 45 22427.5 46 22311.0 47 21805.5 48 21031.5 49 19813.0 50 18872.0 51 18054.5 52 17158.0 53 16002.5 54 14635.0 55 13134.5 56 11554.5 57 9591.0 58 7780.5 59 6709.5 60 5400.0 61 3942.5 62 2941.5 63 2216.0 64 1568.0 65 1132.0 66 826.5 67 600.0 68 445.5 69 331.0 70 271.0 71 222.0 72 165.5 73 103.5 74 80.0 75 62.5 76 44.0 77 40.0 78 39.5 79 40.5 80 36.5 81 23.5 82 11.5 83 10.5 84 8.0 85 5.0 86 4.0 87 4.0 88 5.0 89 3.5 90 1.5 91 1.5 92 2.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04750037208624801 2 0.06840053580419712 3 0.04797537580711049 4 0.06190881828574324 5 0.09595075161422098 6 0.06824220123057631 7 0.08930069952214625 8 0.10228413455905404 9 0.10291747285353736 10-11 0.062462989293416135 12-13 0.046233695497281395 14-15 0.09175488541326907 16-17 0.05122123456633744 18-19 0.027629383096834256 20-21 0.03435860247571939 22-23 0.07623809719842806 24-25 0.16925965920066374 26-27 0.17108050679730324 28-29 0.12635098974941972 30-31 0.14630114602564387 32-33 0.1964140385766355 34-35 0.16799298261169712 36-37 0.16767631346445547 38-39 0.20179741407974364 40-41 0.14669698245969592 42-43 0.07980062510489665 44-45 0.06911304138549085 46-47 0.07663393363248013 48-49 0.11724675176622217 50-51 0.12959684850864664 52-53 0.1524761943968561 54-55 0.22396425438665937 56-57 0.18271809795843402 58-59 0.1380677481973609 60-61 0.23219765221494235 62-63 0.2354435109741693 64-65 0.25278114678564984 66-67 0.13023018680312998 68-69 0.12848850649330085 70-71 0.13672190432158385 72-73 0.15279286354409777 74-75 0.15683039517142883 76-77 0.14440113114219394 78-79 0.1771763878817051 80-81 0.2639437342259181 82-83 0.2819147083318819 84-85 0.21913504989122412 86-87 0.22459759268114265 88-89 0.22333091609217604 90-91 0.1749597038510135 92-93 0.1577804026131538 94-95 0.14851783005633543 96-97 0.1299135176558883 98-99 0.14543030587072933 100-101 0.17100133951049284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 631574.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.29941657120621 #Duplication Level Percentage of deduplicated Percentage of total 1 62.995611927337634 27.276732429998386 2 16.09497743796527 13.938062655812466 3 7.415033192563839 9.631998332824281 4 4.149072986287215 7.186097584703545 5 2.5685678068048494 5.560874372911633 6 1.6240859475998404 4.219318439554059 7 1.1375336945201813 3.4478181711968787 8 0.8237252666685023 2.853345876936592 9 0.6026916247958694 2.348657615341211 >10 2.4634911245705435 17.126319133017397 >50 0.08716614481997724 2.5741617357970195 >100 0.03693480200605684 2.5944719997865917 >500 0.0 0.0 >1k 0.0011080440601816588 1.2421416521199482 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3515 0.5565460262772058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2703 0.4279783524970946 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1549 0.24526025453866057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.166691472416534E-4 0.0 18-19 0.0 0.0 0.0 3.166691472416534E-4 0.0 20-21 0.0 0.0 0.0 4.7500372086248005E-4 0.0 22-23 0.0 0.0 0.0 0.0019000148834499202 0.0 24-25 0.0 0.0 0.0 0.004037531627331081 0.0 26-27 0.0 0.0 0.0 0.005225040929487281 0.0 28-29 0.0 0.0 0.0 0.007995895967851748 0.0 30-31 0.0 0.0 0.0 0.013537606044580684 0.0 32-33 0.0 0.0 0.0 0.019475152555361683 0.0 34-35 0.0 0.0 0.0 0.02770855038364467 0.0 36-37 0.0 0.0 0.0 0.042196163869950315 0.0 38-39 0.0 0.0 0.0 0.05850462495289546 0.0 40-41 0.0 0.0 0.0 0.07615892991161764 0.0 42-43 0.0 0.0 0.0 0.09317989657585651 0.0 44-45 0.0 0.0 0.0 0.11518840230915142 0.0 46-47 0.0 0.0 0.0 0.1369594061820151 0.0 48-49 0.0 0.0 0.0 0.15880957734168918 0.0 50-51 0.0 0.0 0.0 0.17994724292006953 0.0 52-53 0.0 0.0 0.0 0.20021406834353533 0.0 54-55 0.0 0.0 0.0 0.22253924322407193 0.0 56-57 0.0 0.0 0.0 0.24866444787150832 0.0 58-59 0.0 0.0 0.0 0.27589799453429054 0.0 60-61 0.0 0.0 0.0 0.3017065300344852 0.0 62-63 0.0 0.0 0.0 0.3313942625883903 0.0 64-65 0.0 0.0 0.0 0.3600528204137599 0.0 66-67 0.0 0.0 0.0 0.38815720723145664 0.0 68-69 0.0 0.0 0.0 0.4263158394740759 0.0 70-71 0.0 0.0 0.0 0.46779949776273244 0.0 72-73 0.0 0.0 0.0 0.5060372972921621 0.0 74-75 0.0 0.0 0.0 0.5455417734105583 0.0 76-77 0.0 0.0 0.0 0.5886879447222336 0.0 78-79 0.0 0.0 0.0 0.6403050157226231 0.0 80-81 0.0 0.0 0.0 0.692001254009823 0.0 82-83 0.0 0.0 0.0 0.7409266372586585 0.0 84-85 0.0 0.0 0.0 0.7949187268633604 0.0 86-87 0.0 0.0 0.0 0.8501774930570289 0.0 88-89 0.0 0.0 0.0 0.9093154563044077 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1510 0.0 63.509285 1 GTATCAA 2805 0.0 47.559387 1 CCACGCG 25 0.0016178942 37.990345 40-41 ATCAACG 3560 0.0 37.20635 3 CAACGCA 3610 0.0 37.085552 5 TCAACGC 3580 0.0 36.998486 4 TATCAAC 3635 0.0 36.699886 2 AACGCAG 3685 0.0 36.45381 6 CGCAGAG 4115 0.0 32.75989 8 ACGCAGA 4180 0.0 32.25047 7 CATGGGG 1360 0.0 29.322649 4 GCAGAGT 4580 0.0 29.02157 9 GTACATG 3380 0.0 28.934322 1 ACATGGG 3295 0.0 28.384 3 TACATGG 3435 0.0 28.332827 2 GAGTACT 2800 0.0 25.769972 12-13 CAGAGTA 4575 0.0 24.435892 10-11 ATGGGAT 760 0.0 23.737381 5 AGAGTAC 4255 0.0 23.484478 10-11 GTACTTT 3090 0.0 23.197803 14-15 >>END_MODULE