FastQCFastQC Report
Fri 10 Feb 2017
SRR2031430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631574
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41680.6599385028516057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33120.5244041078321781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.30922742228147454No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT7570.1198592722309658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.065.0121841
GTATCAA27700.050.267481
TATCAAC37950.036.545132
ATCAACG37900.036.468033
TCAACGC38550.035.8531344
CAACGCA38700.035.714175
AACGCAG39650.034.858476
AGGCCGC550.002579771234.5426646
GCCTACG600.0039404731.6816651
ATGACGG600.003951179331.6641085
CGCAGAG43450.031.3726068
ACGCAGA43550.031.3005717
GTACATG34500.029.0644841
TACATGG35050.028.7280642
GCAGAGT49200.027.6095569
ACATGGG34450.027.5739673
ATGGGAT7250.026.2047795
CATGGGG13450.024.719194
AGAGTAC46750.024.43385310-11
CATGGGA19150.024.306134
Produced by FastQC (version 0.11.5)