FastQCFastQC Report
Fri 10 Feb 2017
SRR2031429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718320
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25350.3529067824924825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17390.2420926606526339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.14979396369306158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9450.060.7726331
GTATCAA21950.053.1930731
ATCAACG27850.041.4186673
TCAACGC28150.040.808634
TATCAAC29150.039.5687642
CAACGCA29200.039.3411945
AACGCAG30300.038.212986
ACGCAGA33600.034.318697
CGCAGAG35000.032.8103648
GTATCGA650.00587095729.2018496
GTACATG33600.028.9580351
TACATGG33500.028.6214312
GCAGAGT39850.028.5789749
ACATGGG33300.027.7974783
AACCCGA1056.9489484E-527.1160016
GTGTACG1000.00166057223.731461
AGAGTAC36900.023.46766910-11
CATGGGA13900.023.2225114
GAGTACT22550.022.51493312-13
CATGGGG20250.022.5040784
Produced by FastQC (version 0.11.5)