FastQCFastQC Report
Fri 10 Feb 2017
SRR2031429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718320
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.4205646508519879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21840.30404276645506184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13610.1894698741507963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.062.378331
GTATCAA23550.046.434081
TCAACGC29900.036.216784
ATCAACG30050.036.0363
CAACGCA30700.035.273025
TATCAAC31750.034.2561042
AACGCAG31950.034.0416686
ACGCAGA35900.030.031547
CGCAGAG37050.029.0993868
GTACATG32050.028.778861
TACATGG31350.028.1787412
ACATGGG31500.026.9891193
GCAGAGT40950.026.3280169
CATGGGG19700.025.5555884
ACTATAC2101.2641976E-924.8782353
GAGTACT25550.023.32996712-13
CTGGTCG1652.430077E-623.0277889
CAGAGTA39450.022.87456110-11
ATGGGAT7700.022.8221845
GTACTTT26700.022.41796514-15
Produced by FastQC (version 0.11.5)