FastQCFastQC Report
Fri 10 Feb 2017
SRR2031428_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031428_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759163
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9770.1286943647148241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7000.053.5519451
GTATCAA14500.047.123841
TCAACGC17050.038.9704864
CAACGCA17250.038.5186545
AACGCAG17250.038.4957966
ATCAACG17500.037.9683843
TATCAAC19150.034.942492
ACGCAGA21500.030.8861647
CGCAGAG22500.029.7242558
GTACATG24450.028.722951
TACATGG24900.027.2546232
GGACCGT1501.0725635E-625.2972376
ACATGGG26400.024.808893
GCAGAGT27650.024.0195319
TATAATA5250.021.694792
CATGGGG19550.021.606054
GTGTAAC3550.021.3864081
ACGACAG1200.0047850119.7752025
AGAGTAC24050.019.7236810-11
CAGAGTA25350.019.64781810-11
Produced by FastQC (version 0.11.5)