FastQCFastQC Report
Fri 10 Feb 2017
SRR2031428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759163
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.17743225104490076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.11828816736326718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8450.057.9553831
GTATCAA17150.049.0707171
ATCAACG20400.039.349623
TCAACGC20400.039.349624
CAACGCA20450.039.021145
AACGCAG21100.037.8190656
TATCAAC21550.037.690582
ACGCAGA24600.032.631397
CGCAGAG25250.031.6032588
GTACATG24900.027.4964351
GCAGAGT29550.027.0044789
TATTCGT1301.0016955E-525.5763554
TACATGG25650.025.5549892
ACATGGG25400.024.8714963
GTAATAC1652.4285455E-623.029793
GGACCGT1252.2552614E-422.7994966
ACCGTGG1954.2184365E-721.922598
CAGAGTA28450.021.8712210-11
CATGGGG17650.021.7983844
GAGTACT17050.020.75467312-13
Produced by FastQC (version 0.11.5)