FastQCFastQC Report
Fri 10 Feb 2017
SRR2031427_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031427_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187286
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.10747199916448101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21850.043.2354851
GGTATCA12850.043.2236481
TCAACGC23500.039.191414
ATCAACG24450.037.474473
CAACGCA24650.036.977825
AACGCAG26100.035.269446
TATCAAC27550.033.6023832
ACGCAGA28600.031.6887177
CGCAGAG29750.030.4637768
TACCGTA1004.9888426E-528.4700727
GTACCGT1301.0103437E-525.5500666
GCAGAGT35650.025.1558849
GTACATG32400.024.7616981
ACATGGG32350.023.1867493
TACATGG35100.022.4521542
CATGGGG19600.021.07274
CAGAGTA34200.020.94931810-11
AGAGTAC31800.020.59069410-11
TGTACCG1456.1687833E-419.6444685
CCGTATG1456.1898347E-419.6345339
Produced by FastQC (version 0.11.5)