FastQCFastQC Report
Fri 10 Feb 2017
SRR2031427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187286
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15380.12953913378916285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11450.046.488521
GTATCAA21000.043.9051671
TCAACGC23050.038.7403874
ATCAACG23550.037.9178773
CAACGCA23800.037.5195775
AACGCAG24450.036.9106566
TATCAAC26950.033.3104172
ACGCAGA27950.031.608817
CGCAGAG29300.029.9903268
CGTATAC700.00839323927.1418233
GTACATG35800.026.0199361
ACATGGG35250.024.2543953
GCAGAGT36200.024.1427279
TACATGG38950.022.6820622
GTATACG1050.00218923422.6315291
CATGGGG21250.021.4580064
AGAGTAC31300.021.39694810-11
GCCGTAC1404.853114E-420.3563678
GAGTACA25300.019.5364671
CAGAGTA35350.018.67679810-11
Produced by FastQC (version 0.11.5)