FastQCFastQC Report
Fri 10 Feb 2017
SRR2031425_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031425_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825119
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.044.1221161
GGTATCA7550.043.388111
TCAACGC13700.040.8986634
CAACGCA14550.038.5093965
ATCAACG14850.037.7314263
AACGCAG15450.036.246336
TATCAAC16800.033.634532
ACGCAGA16850.033.516427
CGCAGAG18650.029.7726548
GTACATG26150.029.4111441
TACATGG25450.028.9195542
ACATGGG26000.027.577273
TAGATAG2007.203198E-1026.1162225
CATGGGG17850.025.8036524
GTTCGGG1301.0056921E-525.5636771
GCAGAGT23200.023.3185259
GTATTAC2005.409165E-721.363931
CATGGGA9400.021.2162744
GTCTAAC1707.98208E-519.5486931
AGAGTAC19200.019.52585610-11
Produced by FastQC (version 0.11.5)