FastQCFastQC Report
Fri 10 Feb 2017
SRR2031425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825119
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.10955995438233757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.043.6837921
GTATCAA14150.042.3308751
TCAACGC15700.036.9069674
CAACGCA16550.035.2984245
ATCAACG16800.034.7731483
AACGCAG16800.034.7731486
TATCAAC18000.032.7207832
ACGCAGA18750.030.6501267
GTACATG24100.030.3771341
CGCAGAG19600.029.078598
TACATGG24650.027.9399472
ACATGGG24200.027.0839423
CATGGGG17150.024.9245074
GTATAGG2701.8189894E-1224.649461
GCAGAGT24300.022.867989
AGAGTAC20200.021.631410-11
GAGTACA17200.021.2816541
GTATAGT3302.7284841E-1120.1677381
CAGAGTA22550.019.1665110-11
AGAACCG1250.006033377718.998015
Produced by FastQC (version 0.11.5)