##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031425_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 825119 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.311207231926545 34.0 33.0 34.0 31.0 34.0 2 33.41257685255096 34.0 34.0 34.0 31.0 34.0 3 33.50774494345664 34.0 34.0 34.0 31.0 34.0 4 36.67285930877849 37.0 37.0 37.0 35.0 37.0 5 36.577011315943516 37.0 37.0 37.0 35.0 37.0 6 36.619714247278274 37.0 37.0 37.0 37.0 37.0 7 36.628728704586855 37.0 37.0 37.0 37.0 37.0 8 36.6353580513841 37.0 37.0 37.0 37.0 37.0 9 38.5794885343811 39.0 39.0 39.0 38.0 39.0 10-11 38.58518286453227 39.0 39.0 39.0 38.0 39.0 12-13 38.58921440422533 39.0 39.0 39.0 38.0 39.0 14-15 40.32977182685164 41.0 41.0 41.0 39.0 41.0 16-17 40.38220123400382 41.0 41.0 41.0 39.0 41.0 18-19 40.38781193985352 41.0 41.0 41.0 39.0 41.0 20-21 40.38088324229596 41.0 41.0 41.0 39.0 41.0 22-23 40.34472845734979 41.0 41.0 41.0 39.0 41.0 24-25 40.321742681964665 41.0 41.0 41.0 39.0 41.0 26-27 40.25560070729192 41.0 40.5 41.0 39.0 41.0 28-29 40.19713277721153 41.0 40.0 41.0 39.0 41.0 30-31 40.092193974444896 41.0 40.0 41.0 38.0 41.0 32-33 40.008155793285574 41.0 40.0 41.0 38.0 41.0 34-35 39.95069014287636 41.0 40.0 41.0 38.0 41.0 36-37 39.88430032516521 41.0 40.0 41.0 38.0 41.0 38-39 39.801318355291784 41.0 40.0 41.0 38.0 41.0 40-41 39.71590764483668 41.0 40.0 41.0 37.5 41.0 42-43 39.5500121800613 41.0 40.0 41.0 37.0 41.0 44-45 39.45535128872321 41.0 40.0 41.0 37.0 41.0 46-47 39.3466300012483 41.0 40.0 41.0 36.5 41.0 48-49 39.18120537764855 41.0 39.0 41.0 36.0 41.0 50-51 39.104478869108576 41.0 39.0 41.0 35.5 41.0 52-53 39.122464153655415 41.0 39.0 41.0 35.0 41.0 54-55 39.050954468385775 41.0 39.0 41.0 35.0 41.0 56-57 38.97421038662302 41.0 39.0 41.0 35.0 41.0 58-59 38.92662876506297 41.0 39.0 41.0 35.0 41.0 60-61 38.737485744480495 41.0 37.5 41.0 35.0 41.0 62-63 38.53735885369262 40.0 37.0 41.0 35.0 41.0 64-65 38.27363507566787 40.0 37.0 41.0 35.0 41.0 66-67 37.9903832053316 39.0 36.0 41.0 35.0 41.0 68-69 37.659409127653106 39.0 35.5 41.0 35.0 41.0 70-71 37.323264886640594 38.5 35.0 41.0 35.0 41.0 72-73 36.9863680269149 37.0 35.0 40.0 35.0 41.0 74-75 36.613865393961355 37.0 35.0 39.0 34.0 41.0 76-77 35.43250428120065 35.5 34.5 39.0 32.0 39.5 78-79 35.81196833426451 36.0 35.0 38.5 34.0 39.5 80-81 35.57651926570591 35.5 35.0 37.0 34.0 39.0 82-83 35.27247100115256 35.0 35.0 37.0 34.0 39.0 84-85 34.98453798785387 35.0 35.0 36.5 34.0 38.0 86-87 34.76608040779573 35.0 35.0 36.0 34.0 37.0 88-89 34.577311878650235 35.0 35.0 36.0 34.0 37.0 90-91 34.4177355023942 35.0 35.0 35.5 33.5 36.5 92-93 34.31108603728674 35.0 35.0 35.0 33.0 36.0 94-95 34.21587431631074 35.0 35.0 35.0 33.0 36.0 96-97 34.14629829151916 35.0 35.0 35.0 33.0 36.0 98-99 34.0838066993973 35.0 35.0 35.0 33.0 36.0 100-101 33.84176767229938 35.0 34.5 35.0 32.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1052078208788032 1101 2 -0.05767264176319742 1101 3 -0.04502977213597603 1101 4 0.02282257860754555 1101 5 -0.025523276494695324 1101 6 -0.07623198341097037 1101 7 -0.06011634194791782 1101 8 -0.028702889155397315 1101 9 0.01866153720203556 1101 10-11 0.024233434077522986 1101 12-13 0.06566897913106828 1101 14-15 0.100656620883953 1101 16-17 -0.018713962614668844 1101 18-19 -0.02372498408026047 1101 20-21 -0.03411219171717761 1101 22-23 0.03766416421718333 1101 24-25 -0.05683230073058354 1101 26-27 -0.07310857402407578 1101 28-29 -0.07093813487110623 1101 30-31 -0.04571523251852483 1101 32-33 -0.04587673271288395 1101 34-35 -0.09789451464623511 1101 36-37 -0.0441834211009251 1101 38-39 -0.0296731752442696 1101 40-41 -0.07588595261907471 1101 42-43 -0.05664040219866706 1101 44-45 -0.16745235140302128 1101 46-47 -0.1727429917898533 1101 48-49 -0.11832388898638868 1101 50-51 -0.08349474007404467 1101 52-53 -0.14770885360333352 1101 54-55 -0.14035074223714616 1101 56-57 -0.13132399395750838 1101 58-59 0.018874647411138312 1101 60-61 -0.07839819048322028 1101 62-63 -0.1410651313234581 1101 64-65 -0.09369154885699515 1101 66-67 -0.09992491992387897 1101 68-69 -0.03562435733783076 1101 70-71 -0.03692410281398395 1101 72-73 0.0037851353005819988 1101 74-75 -0.0650015380553981 1101 76-77 -0.14427177975900207 1101 78-79 -0.03825077907884378 1101 80-81 0.08347448869858454 1101 82-83 0.043808428054113335 1101 84-85 0.02690570754703714 1101 86-87 0.05491062470587593 1101 88-89 -0.008166108492659419 1101 90-91 -0.04858505002179925 1101 92-93 0.10389633903489681 1101 94-95 0.03901836391882796 1101 96-97 0.14220605435409794 1101 98-99 0.08050497181647387 1101 100-101 0.1150136185968691 1102 1 0.0949058968796237 1102 2 0.08465547089122794 1102 3 0.029769191112514193 1102 4 0.03345223531282926 1102 5 0.11920841605383714 1102 6 0.1025448861015903 1102 7 0.05067355341697777 1102 8 0.09181578197136986 1102 9 0.06556642041980609 1102 10-11 0.03371668286096252 1102 12-13 0.1162567670252912 1102 14-15 0.09898777585762275 1102 16-17 0.07529702517913961 1102 18-19 0.0491540689609522 1102 20-21 0.08434442200427839 1102 22-23 -0.004450173819932957 1102 24-25 0.009387986812889437 1102 26-27 -0.00463977625707912 1102 28-29 0.014254188441775284 1102 30-31 0.023436610226063692 1102 32-33 0.124279001542007 1102 34-35 0.10433848861023165 1102 36-37 0.08847868208685838 1102 38-39 0.14418023222713572 1102 40-41 0.16461972219244103 1102 42-43 0.2621053056943836 1102 44-45 0.20334876805232227 1102 46-47 0.2920580390542469 1102 48-49 0.2511591382689531 1102 50-51 0.2278759281285474 1102 52-53 0.21871339372540177 1102 54-55 0.23857122818146337 1102 56-57 0.18389572499717843 1102 58-59 0.19222223238562464 1102 60-61 0.20714414722898056 1102 62-63 0.21254171537171374 1102 64-65 0.2219084600041228 1102 66-67 0.2972011221812352 1102 68-69 0.24563879589316429 1102 70-71 0.2936124382462424 1102 72-73 0.28095186473354516 1102 74-75 0.27581944508630585 1102 76-77 0.05209442096022343 1102 78-79 0.1955407482097229 1102 80-81 0.2498402463616145 1102 82-83 0.09045855395591218 1102 84-85 0.20339529941843892 1102 86-87 0.20785731766054028 1102 88-89 0.2176577914357125 1102 90-91 0.20274596465073813 1102 92-93 0.17635633475202184 1102 94-95 0.19505721994771363 1102 96-97 0.2540888216831334 1102 98-99 0.28294842676873344 1102 100-101 0.18966120430977895 1103 1 0.03307049781893312 1103 2 0.10382362606997475 1103 3 0.126890511819866 1103 4 0.06593878413541887 1103 5 0.038374024694604714 1103 6 0.0851684022884811 1103 7 0.10226095605737129 1103 8 0.07307690033809422 1103 9 0.0684913011872581 1103 10-11 -0.017511476028452932 1103 12-13 0.010613638858039565 1103 14-15 0.08946645223993954 1103 16-17 0.030503422082546194 1103 18-19 0.05757492437944478 1103 20-21 0.08540760161159966 1103 22-23 0.07255440631052323 1103 24-25 0.011871770822814653 1103 26-27 0.044275580458318586 1103 28-29 0.09867374148797836 1103 30-31 0.1716305691103841 1103 32-33 0.11472493672170714 1103 34-35 0.1071023917981293 1103 36-37 0.08228059053518422 1103 38-39 0.016991573779719715 1103 40-41 0.06301831359314747 1103 42-43 0.08035798046874021 1103 44-45 0.1346677292103422 1103 46-47 0.09918507128249843 1103 48-49 0.04048587000573178 1103 50-51 0.14664318547075794 1103 52-53 0.29304774688656465 1103 54-55 0.20880947499920666 1103 56-57 0.05589763126275216 1103 58-59 0.0939385989852326 1103 60-61 0.13372836669690713 1103 62-63 0.04951508222233514 1103 64-65 -0.007294509986742526 1103 66-67 0.005416981292199807 1103 68-69 0.14097153745957058 1103 70-71 0.002377621978965294 1103 72-73 0.01268227367116026 1103 74-75 -0.04957717820433061 1103 76-77 -0.1270690776919281 1103 78-79 0.015776994176192716 1103 80-81 0.014470107065989168 1103 82-83 0.0806398310810934 1103 84-85 -0.05603052902494454 1103 86-87 -0.04051380750907185 1103 88-89 -0.04781970262539659 1103 90-91 0.08617909802021728 1103 92-93 0.08368872707894326 1103 94-95 0.1300204588529894 1103 96-97 0.005910505817837475 1103 98-99 0.010235126928435534 1103 100-101 0.11177443066426207 1104 1 -0.04836802318146738 1104 2 -0.02997685525119209 1104 3 -0.013633158806783285 1104 4 0.03310859866520133 1104 5 0.0264022236520276 1104 6 0.020441760396529673 1104 7 0.02617989681064614 1104 8 0.04274516290474395 1104 9 0.014163237441970011 1104 10-11 0.067656008430653 1104 12-13 0.10908378589059708 1104 14-15 0.07472407811903281 1104 16-17 0.018645573401073534 1104 18-19 0.030994260564419562 1104 20-21 -0.013098261832652724 1104 22-23 -0.004577735908831926 1104 24-25 -0.009688185977339003 1104 26-27 0.05060588923520726 1104 28-29 -0.006914176160186969 1104 30-31 0.012214617480609036 1104 32-33 0.020036477374631545 1104 34-35 0.03215830499024008 1104 36-37 0.01996395604163581 1104 38-39 -0.02142312777564115 1104 40-41 -0.026511675436815096 1104 42-43 0.06371328222989803 1104 44-45 0.08321738777845411 1104 46-47 0.13961179736313767 1104 48-49 0.1258081255821608 1104 50-51 0.15339528806190827 1104 52-53 0.09565589533077201 1104 54-55 0.19577978785796546 1104 56-57 0.18827796002381803 1104 58-59 0.16166286828697451 1104 60-61 0.19496500493216473 1104 62-63 0.17144155743770284 1104 64-65 0.16791064678279355 1104 66-67 0.11789221366147018 1104 68-69 0.13220485917089775 1104 70-71 0.1349390839707496 1104 72-73 0.2295183098297784 1104 74-75 0.188615571364096 1104 76-77 0.07698898698879475 1104 78-79 0.2522460016851369 1104 80-81 0.17558088746957878 1104 82-83 0.12650395564632788 1104 84-85 0.3294413953243023 1104 86-87 0.2748612573332281 1104 88-89 0.2256004839503376 1104 90-91 0.25160050915862797 1104 92-93 0.18376101029307534 1104 94-95 0.1923532507708856 1104 96-97 0.2292341730429328 1104 98-99 0.2607934084588024 1104 100-101 0.11941093964014016 1105 1 -0.12410458620114184 1105 2 -0.0024675207464710525 1105 3 0.011380176199956793 1105 4 -0.0311677026745798 1105 5 -0.025907085751505576 1105 6 -0.044349115321210775 1105 7 -0.056318299041379305 1105 8 -0.007179533527754245 1105 9 0.005211525854633692 1105 10-11 0.04760408034555752 1105 12-13 0.09315472812648551 1105 14-15 0.08361382114560456 1105 16-17 -0.006183424747717936 1105 18-19 0.0038308518229257515 1105 20-21 -0.005506720933404097 1105 22-23 -0.010216874938038245 1105 24-25 0.0842326197708374 1105 26-27 -0.007822619440389644 1105 28-29 0.09614738450625282 1105 30-31 0.09497088493377959 1105 32-33 0.16465933200737481 1105 34-35 0.05528042227555119 1105 36-37 0.016877294186031122 1105 38-39 -0.05923650043416728 1105 40-41 -0.004859546542206772 1105 42-43 -0.050172381853435866 1105 44-45 0.06763582460190065 1105 46-47 -0.10351047680460113 1105 48-49 -0.13890274009509795 1105 50-51 -0.16213058185118 1105 52-53 -0.130649366506006 1105 54-55 -0.17130078695927153 1105 56-57 -0.19581729898907696 1105 58-59 -0.22575202299349684 1105 60-61 -0.14844671732317494 1105 62-63 -0.11659145062606058 1105 64-65 -0.24151484975010362 1105 66-67 -0.24841571517665528 1105 68-69 -0.15295484714518892 1105 70-71 -0.13496066815500996 1105 72-73 -0.10569395210113441 1105 74-75 -0.1413662302737606 1105 76-77 -0.3868292176486534 1105 78-79 -0.12064628283683732 1105 80-81 -0.07333662374751526 1105 82-83 -0.10762267985302287 1105 84-85 -0.05844179001660876 1105 86-87 -0.01696516794368108 1105 88-89 -0.08601430692538514 1105 90-91 -0.01080653800023157 1105 92-93 0.032856583070838496 1105 94-95 0.011799612667275028 1105 96-97 -0.09948864707568106 1105 98-99 -0.1842483222681892 1105 100-101 -0.24353264543719888 1106 1 -0.08170966774780908 1106 2 0.008096147597299819 1106 3 -0.00937025183970519 1106 4 0.0019958756785953824 1106 5 0.06982917169170832 1106 6 -0.07781363623817583 1106 7 0.01355588943631858 1106 8 0.05837481436185499 1106 9 0.09963444657525855 1106 10-11 0.03479868207875825 1106 12-13 0.08337336435997145 1106 14-15 0.07919098807815317 1106 16-17 0.028164201896515806 1106 18-19 -0.029296934817558906 1106 20-21 0.005641301903175133 1106 22-23 -0.007041638712735221 1106 24-25 0.007195706097526511 1106 26-27 0.05162741532870285 1106 28-29 0.002083111355588585 1106 30-31 -0.01339984708709352 1106 32-33 -0.18612063515190158 1106 34-35 0.003203480319477592 1106 36-37 -0.04366986614331125 1106 38-39 -0.00784106190533862 1106 40-41 0.12169045374997012 1106 42-43 0.17297652920196782 1106 44-45 0.02393487385713655 1106 46-47 -0.3209385573753991 1106 48-49 0.06878853373925864 1106 50-51 -0.011382462011759742 1106 52-53 -0.2601532787593328 1106 54-55 -0.019062563163188884 1106 56-57 0.10770811166604233 1106 58-59 0.0869652838335142 1106 60-61 0.16366943481936147 1106 62-63 0.03376296037383497 1106 64-65 -0.036245725288445385 1106 66-67 -0.010513314064041879 1106 68-69 -0.16105473085864475 1106 70-71 -0.06330346803112263 1106 72-73 0.11655505303534852 1106 74-75 0.13100285971098913 1106 76-77 -0.06388716808370987 1106 78-79 0.17236220783394174 1106 80-81 0.2470839361809638 1106 82-83 0.11370012341234315 1106 84-85 0.0890193358007636 1106 86-87 0.10277491117792437 1106 88-89 -0.22962161486267263 1106 90-91 -0.09576218549423032 1106 92-93 -0.04882960103588374 1106 94-95 -0.006774825329792122 1106 96-97 0.11082856976074851 1106 98-99 0.009829683213055773 1106 100-101 0.0011080934206191273 1107 1 -0.0793065219128195 1107 2 -0.03240187789221949 1107 3 -0.03885339427348811 1107 4 -0.03612130695351823 1107 5 -0.0326725254254967 1107 6 -0.05011891381963807 1107 7 -0.09599633647054162 1107 8 -0.05280971071714902 1107 9 -0.07134575535032894 1107 10-11 -0.021612517919010088 1107 12-13 -0.012871650576052218 1107 14-15 -0.0029223026843681055 1107 16-17 -0.005188507148595534 1107 18-19 -0.029816176014506368 1107 20-21 -0.007534182106290643 1107 22-23 0.014910763945223948 1107 24-25 -0.031257415134497535 1107 26-27 -0.07118114288137178 1107 28-29 -0.06624415138603723 1107 30-31 0.11495100105332767 1107 32-33 0.055164127871989876 1107 34-35 0.07172659108303492 1107 36-37 0.09880713042620926 1107 38-39 0.11919600146160292 1107 40-41 0.06805597446177103 1107 42-43 0.27608389155426494 1107 44-45 0.21621790421846043 1107 46-47 0.11661449931737167 1107 48-49 0.22637022187951317 1107 50-51 0.3004048010365281 1107 52-53 0.2662764466491865 1107 54-55 0.15575793271563754 1107 56-57 0.15335464013788425 1107 58-59 0.21972793972784643 1107 60-61 0.2146533446149661 1107 62-63 0.2928775600423563 1107 64-65 0.25545770644284715 1107 66-67 0.22838642429415046 1107 68-69 0.2480041810627185 1107 70-71 0.274514854701323 1107 72-73 0.15441296355300693 1107 74-75 0.16109306131596668 1107 76-77 -0.022064206331918967 1107 78-79 0.1924669481924326 1107 80-81 0.199453139671931 1107 82-83 0.21470891496732492 1107 84-85 0.1958540832822635 1107 86-87 0.23742983532689266 1107 88-89 0.22662064007987937 1107 90-91 0.1902818656743861 1107 92-93 0.17719922835051705 1107 94-95 0.17160508637829963 1107 96-97 0.24949733290730336 1107 98-99 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-0.782859647914492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 4.0 14 10.0 15 35.0 16 87.0 17 188.0 18 367.0 19 661.0 20 895.0 21 1416.0 22 1970.0 23 2169.0 24 2011.0 25 1802.0 26 1753.0 27 1870.0 28 2353.0 29 2939.0 30 3446.0 31 4357.0 32 5765.0 33 7823.0 34 12123.0 35 21978.0 36 52731.0 37 164578.0 38 349596.0 39 181946.0 40 245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74361136216131 19.60015285418802 13.66140371096001 23.99483207269066 2 14.487227810009054 20.914832271458575 41.40960086679037 23.188339051742005 3 17.48172081845164 26.655670273015165 31.008860540116036 24.85374836841716 4 11.757940369813324 17.777193350292503 40.458648994872256 30.00621728502192 5 12.598213344289356 38.61791721658868 36.17317301667521 12.610696422446757 6 29.271898962452685 39.06563780497116 19.160751358288923 12.501711874287224 7 26.172709633398334 32.96009424095191 24.01180920570245 16.855386919947303 8 24.805876485694792 36.23053159604857 21.476538535653646 17.487053382602994 9 26.2693017613217 15.866196269871377 20.968611800237298 36.89589016856962 10-11 23.656890703037988 26.513205973926183 30.393737145793516 19.436166177242313 12-13 24.72873609745988 24.153364544992904 29.72092510292455 21.396974254622666 14-15 21.97319416956827 25.666843206858648 26.993257942187732 25.36670468138535 16-17 21.74662079045568 28.752095152335606 27.454161157360335 22.047122899848386 18-19 21.77131699796747 28.228501201700197 28.773114397253117 21.227067403079214 20-21 22.728217534082873 27.39325487268274 28.521927148067867 21.356600445166528 22-23 22.5120861354544 27.195531796019722 28.180844217621942 22.111537850903932 24-25 22.135473792265113 27.40937973795295 28.352274035623953 22.102872434157984 26-27 22.159448305952647 27.599852138238628 28.17269526539368 22.068004290415043 28-29 22.192071689053336 27.557358393152988 28.302463038664726 21.94810687912895 30-31 22.55080782287161 27.437921075626665 28.140546999887288 21.87072410161443 32-33 22.35950208394183 27.45579728499768 28.082797754020934 22.101902877039553 34-35 22.34665545212266 27.509728899710222 28.1548479673841 21.988767680783013 36-37 22.56713936056944 27.401621755594974 28.012946411527906 22.018292472307685 38-39 22.391898213796598 27.62815991235023 27.983631114698547 21.996310759154625 40-41 22.34229244508974 27.55893392347043 28.088372707451896 22.010400923987934 42-43 22.53432535185834 27.37120342641486 27.988993102813048 22.10547811891376 44-45 22.621161311277525 27.419317698416833 27.772418281484246 22.187102708821392 46-47 22.44953002984438 27.371670388122375 27.830306468544606 22.34849311348864 48-49 22.394354674996894 27.379462676075544 27.90236423738725 22.323818411540312 50-51 22.436884861456345 27.590323335179534 27.63904358038053 22.333748222983594 52-53 22.483363005820976 27.64637585608864 27.623167082566276 22.247094055524112 54-55 22.46597157500918 27.425983403606025 27.796778404084744 22.31126661730005 56-57 22.541839419526152 27.56862949465471 27.57220473652891 22.317326349290223 58-59 22.514631222890273 27.494094791175577 27.646921231967752 22.344352753966398 60-61 22.562948059896858 27.651211693340688 27.675512219953095 22.11032802680936 62-63 22.514356914469396 27.66090774000713 27.63284842637551 22.191886919147965 64-65 22.504858805270235 27.602801532273556 27.655889320446008 22.236450342010198 66-67 22.494768679586247 27.639915474709376 27.46478062390427 22.40053522180011 68-69 22.429350388380463 27.56846803936041 27.616280831372947 22.38590074088618 70-71 22.4505749216016 27.305185656501386 27.79681558575346 22.447423836143557 72-73 22.49452182302095 27.456635138869363 27.620556253120863 22.428286784988828 74-75 22.36220644218288 27.707785667797445 27.42490846486396 22.50509942515571 76-77 22.423196443548115 27.58104550765248 27.506811361642097 22.48894668715731 78-79 22.454094500308443 27.53469499550974 27.51469787994217 22.496512624239656 80-81 22.48930154317135 27.545420721132345 27.356781264278247 22.608496471418064 82-83 22.485605106657342 27.546147888971166 27.463371949985394 22.504875054386094 84-85 22.59062026204705 27.537664264184926 27.397745052531818 22.47397042123621 86-87 22.39178781139599 27.533798319022196 27.490471025251022 22.58394284433079 88-89 22.505244812453263 27.48857412607669 27.434398940250954 22.5717821212191 90-91 22.545942192160346 27.530047399568303 27.426364631268683 22.497645777002663 92-93 22.5889235370898 27.56026706450827 27.419560087696443 22.431249310705486 94-95 22.385977500634812 27.477454273733994 27.608598556079635 22.52796966955156 96-97 22.45300374855021 27.405198522879733 27.6973987994459 22.444398929124162 98-99 22.522024095918287 27.432649108795214 27.42810430980259 22.61722248548391 100-101 22.453124943190012 27.500336315125455 27.545057137212936 22.5014816044716 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14.0 1 10.0 2 14.0 3 25.0 4 18.5 5 15.5 6 27.5 7 58.5 8 125.5 9 157.5 10 133.5 11 131.5 12 143.0 13 163.0 14 192.5 15 216.0 16 232.0 17 253.0 18 312.5 19 358.5 20 384.0 21 454.0 22 596.5 23 776.0 24 1030.5 25 1391.0 26 2102.5 27 3299.0 28 4995.0 29 7546.5 30 10704.5 31 14460.0 32 18479.5 33 21660.0 34 24539.5 35 26651.0 36 28085.5 37 28645.5 38 28152.0 39 28070.5 40 28020.5 41 27476.0 42 27109.0 43 27707.0 44 27923.0 45 28394.5 46 29216.0 47 29327.5 48 29362.5 49 29216.5 50 28566.5 51 27414.0 52 26263.5 53 25552.0 54 24807.0 55 23403.5 56 21742.5 57 19975.5 58 18502.0 59 16639.0 60 13534.0 61 10574.5 62 8399.5 63 6161.5 64 4252.5 65 3148.0 66 2254.5 67 1662.5 68 1257.0 69 812.0 70 542.0 71 426.5 72 313.5 73 187.0 74 132.0 75 90.5 76 52.0 77 29.0 78 19.0 79 11.0 80 8.0 81 4.5 82 5.0 83 4.0 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0974404904019905 2 7.271678388208247E-4 3 0.0 4 0.0 5 2.4238927960694155E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.00460539631253189 20-21 0.004181215073219741 22-23 0.0 24-25 0.0 26-27 0.0029086713552832987 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.003090463314988505 38-39 0.002060308876659003 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.020966672686000443 48-49 0.001393738357739914 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.004120617753318006 62-63 0.009816765824081133 64-65 0.00854422210614469 66-67 0.0049083829120405665 68-69 0.0031510606348902396 70-71 7.877651587225599E-4 72-73 0.0032722552746937113 74-75 0.0031510606348902396 76-77 0.0032722552746937113 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 4.847785592138831E-4 88-89 0.002181503516462474 90-91 7.271678388208247E-4 92-93 0.0 94-95 0.0089078060255551 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 825119.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.59854370043326 #Duplication Level Percentage of deduplicated Percentage of total 1 73.37620993838516 45.19867674461784 2 15.118500026238152 18.625551691024643 3 5.346383381551637 9.879882911033361 4 2.3506284818785366 5.791811650579125 5 1.2632757062760172 3.8907971899369467 6 0.7330329032117414 2.7092255593406347 7 0.4866776647950405 2.098504478203148 8 0.3132557743447954 1.5436879604312752 9 0.18856163693439446 1.0453610009635674 >10 0.8011811566090438 7.878081071887381 >50 0.0183480178601724 0.7755727615572086 >100 0.003550780729538794 0.3735301432828451 >500 3.945311857736613E-4 0.18931683714200792 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 904 0.10955995438233757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2119463980347077E-4 0.0 3 0.0 0.0 0.0 1.2119463980347077E-4 0.0 4 0.0 0.0 0.0 1.2119463980347077E-4 0.0 5 0.0 0.0 0.0 1.2119463980347077E-4 0.0 6 0.0 0.0 0.0 1.2119463980347077E-4 0.0 7 0.0 0.0 0.0 1.2119463980347077E-4 0.0 8 0.0 0.0 0.0 1.2119463980347077E-4 0.0 9 0.0 0.0 0.0 1.2119463980347077E-4 0.0 10-11 0.0 0.0 0.0 1.2119463980347077E-4 0.0 12-13 0.0 0.0 0.0 1.2119463980347077E-4 0.0 14-15 0.0 0.0 0.0 1.2119463980347077E-4 0.0 16-17 0.0 0.0 0.0 1.8179195970520617E-4 0.0 18-19 0.0 0.0 0.0 2.4238927960694155E-4 0.0 20-21 0.0 0.0 0.0 3.0298659950867696E-4 0.0 22-23 0.0 0.0 0.0 3.6358391941041234E-4 0.0 24-25 0.0 0.0 0.0 4.847785592138831E-4 0.0 26-27 0.0 0.0 0.0 6.059731990173539E-4 0.0 28-29 0.0 0.0 0.0 8.483624786242954E-4 0.0 30-31 0.0 0.0 0.0 0.0016967249572485909 0.0 32-33 0.0 0.0 0.0 0.0027268793955780926 0.0 34-35 0.0 0.0 0.0 0.004787188272237095 0.0 36-37 0.0 0.0 0.0 0.008301832826537748 0.0 38-39 0.0 0.0 0.0 0.012301255940052283 0.0 40-41 0.0 0.0 0.0 0.019269947728751853 0.0 42-43 0.0 0.0 0.0 0.025511471678630597 0.0 44-45 0.0 0.0 0.0 0.03241956614742843 0.0 46-47 0.0 0.0 0.0 0.03999423113514536 0.0 48-49 0.0 0.0 0.0 0.048417258601486574 0.0 50-51 0.0 0.0 0.0 0.05690088338772953 0.0 52-53 0.0 0.0 0.0 0.06368778321672389 0.0 54-55 0.0 0.0 0.0 0.07386813296021544 0.0 56-57 0.0 0.0 0.0 0.08410908002360871 0.0 58-59 0.0 0.0 0.0 0.09634973864375926 0.0 60-61 0.0 0.0 0.0 0.10901457850322196 0.0 62-63 0.0 0.0 0.0 0.12180061300248812 0.0 64-65 0.0 0.0 0.0 0.1341018689425404 0.0 66-67 0.0 0.0 0.0 0.14706969540151177 0.0 68-69 0.0 0.0 0.0 0.1622190253769456 0.0 70-71 0.0 0.0 0.0 0.18039822134746625 0.0 72-73 0.0 0.0 0.0 0.19984996103592328 0.0 74-75 0.0 0.0 0.0 0.21772617040693526 0.0 76-77 0.0 0.0 0.0 0.2369961181356871 0.0 78-79 0.0 0.0 0.0 0.25796279082168755 0.0 80-81 0.0 0.0 0.0 0.281898732182873 0.0 82-83 0.0 0.0 0.0 0.3106218618162956 0.0 84-85 0.0 0.0 0.0 0.33813304505168346 0.0 86-87 0.0 0.0 0.0 0.36540183900746437 0.0 88-89 0.0 0.0 0.0 0.3938219820413783 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 740 0.0 43.683792 1 GTATCAA 1415 0.0 42.330875 1 TCAACGC 1570 0.0 36.906967 4 CAACGCA 1655 0.0 35.298424 5 ATCAACG 1680 0.0 34.773148 3 AACGCAG 1680 0.0 34.773148 6 TATCAAC 1800 0.0 32.720783 2 ACGCAGA 1875 0.0 30.650126 7 GTACATG 2410 0.0 30.377134 1 CGCAGAG 1960 0.0 29.07859 8 TACATGG 2465 0.0 27.939947 2 ACATGGG 2420 0.0 27.083942 3 CATGGGG 1715 0.0 24.924507 4 GTATAGG 270 1.8189894E-12 24.64946 1 GCAGAGT 2430 0.0 22.86798 9 AGAGTAC 2020 0.0 21.6314 10-11 GAGTACA 1720 0.0 21.281654 1 GTATAGT 330 2.7284841E-11 20.167738 1 CAGAGTA 2255 0.0 19.16651 10-11 AGAACCG 125 0.0060333777 18.99801 5 >>END_MODULE