FastQCFastQC Report
Fri 10 Feb 2017
SRR2031423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24113
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3321.3768506614689173No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3131.2980549910836479No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3081.2773192883506823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2851.1819350557790402No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT2110.8750466553311492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.5971882387094098No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC840.34835980591382243No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG810.335918384274043TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT790.3276241031808568TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT650.2695641355285531RNA PCR Primer, Index 11 (95% over 23bp)
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC570.23638701115580807No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG540.2239455895160287No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.2197984489694356No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC490.20320988678306307No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.19076846514328372No Hit
GGAGAAGAAGGAGAAGAAGGAGAAGAAGGAGAAGAAGGAGAAGAAGGAGA430.17832704350350434No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC430.17832704350350434No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.17832704350350434No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA420.17417990295691121RNA PCR Primer, Index 11 (96% over 28bp)
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG410.1700327624103181No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA400.16588562186372496No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT390.16173848131713184No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG370.1534442002239456No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT360.14929705967735246No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT360.14929705967735246No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT360.14929705967735246No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC360.14929705967735246No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA360.14929705967735246No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.14514991913075934No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT350.14514991913075934TruSeq Adapter, Index 11 (96% over 26bp)
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA350.14514991913075934No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC350.14514991913075934No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA330.13685563803757309No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA320.13270849749097996No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG310.12856135694438686No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA300.12441421639779374No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG300.12441421639779374No Hit
TCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAA290.12026707585120058No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT280.11611993530460747No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG280.11611993530460747No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC280.11611993530460747No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC280.11611993530460747No Hit
GAAGAAGGAGAAGAAGGAGAAGAAGGAGAAGAAGGAGAAGAAGGAGAAGA270.11197279475801435No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG260.10782565421142123No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT260.10782565421142123No Hit
AAGGAGAAGAAGGAGAAGAAGGAGAAGAAGGAGAAGAAGGAGAAGAAGGA250.1036785136648281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT156.5394683E-494.9979255
GATGACC156.5394683E-494.9979259
GGATGAC200.002049626571.248448
CCTTGGC200.002049626571.248449
GGGATGA200.002049626571.248447
CCCTTGG200.002049626571.248448
TGACCTT301.2757938E-463.331955
ATCAATA250.004962416456.9987562
ATACACA651.2901607E-436.5376634
GGTATCA2100.036.2649231
CTTATAC752.9545522E-431.7318081
TACACAT752.9909567E-431.6659745
TATACAC804.3663936E-429.686853
TTATACA804.3663936E-429.686852
GTATCAA2700.028.1475322
ACCTCAA350.00831432627.14226560-61
ACATCTC908.6960226E-426.3883138
ACACATC908.6960226E-426.3883136
TATCAAC3150.025.6343613
AACGCAG3200.025.2338227
Produced by FastQC (version 0.11.5)