FastQCFastQC Report
Fri 10 Feb 2017
SRR2031421_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031421_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750478
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC10520.14017732698360244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.13071668989630608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.10619898251514369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGA301.3107409E-463.2372449
GGTATCA7300.057.2001041
GTATCAA16050.046.7109871
TCAACGC18400.039.974214
ATCAACG18900.039.1677633
CAACGCA19000.038.7118645
AACGCAG19800.037.13046
TATCAAC20250.036.3222472
ACGCAGA22500.032.463957
CGCAGAG23400.031.6207318
CATGGGG12000.028.0765084
GTACATG30800.027.5765111
TCGCTAG350.00835346527.16872284-85
TCTAGCG350.00838894427.14512828-29
CCGCTAC700.00845210727.1016759
GCAGAGT27050.026.6508169
ACATGGG29650.026.5674213
TAATACT9050.025.692924
TACATGG34400.024.0025182
ATGGGGG5600.023.7266255
Produced by FastQC (version 0.11.5)