FastQCFastQC Report
Fri 10 Feb 2017
SRR2031421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750478
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.16322930185828233No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC11990.1597648432065963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.1268524860155794No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT8240.1097966895765099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.062.14831
GTATCAA17850.047.4102751
TCAACGC20400.040.9768834
CAACGCA21050.039.93725
AACGCAG21250.039.337816
ATCAACG21250.039.11433
TATCAAC21800.038.3453452
ACGCAGA23850.034.451987
CGCAGAG24450.033.606538
GCAGAGT29100.027.9099839
GTACATG29850.027.0766741
ACATGGG27100.026.8150483
TCTGACG458.953301E-426.38663186-87
CATGGGG11100.026.101374
TACATGG34000.022.6304172
AGAGTAC25400.022.62601710-11
GTCTTAC1703.1216932E-622.373391
CAGAGTA27400.022.18788110-11
CATGGGA15250.022.1128644
GAGTACT13350.021.52509112-13
Produced by FastQC (version 0.11.5)