##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031421_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 750478 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.35312560794587 34.0 33.0 34.0 31.0 34.0 2 33.44479784883767 34.0 34.0 34.0 31.0 34.0 3 33.5338784081612 34.0 34.0 34.0 31.0 34.0 4 36.6936805609225 37.0 37.0 37.0 35.0 37.0 5 36.62003949482863 37.0 37.0 37.0 35.0 37.0 6 36.66358507511213 37.0 37.0 37.0 37.0 37.0 7 36.67135612236468 37.0 37.0 37.0 37.0 37.0 8 36.67947095051421 37.0 37.0 37.0 37.0 37.0 9 38.62126404771359 39.0 39.0 39.0 38.0 39.0 10-11 38.62668126180914 39.0 39.0 39.0 38.5 39.0 12-13 38.62981126695253 39.0 39.0 39.0 39.0 39.0 14-15 40.36181606922521 41.0 41.0 41.0 39.0 41.0 16-17 40.414490498055905 41.0 41.0 41.0 39.0 41.0 18-19 40.444803178774066 41.0 41.0 41.0 39.5 41.0 20-21 40.44039398889774 41.0 41.0 41.0 39.0 41.0 22-23 40.39969526088706 41.0 41.0 41.0 39.0 41.0 24-25 40.38145821729618 41.0 41.0 41.0 39.0 41.0 26-27 40.320983426562805 41.0 41.0 41.0 39.0 41.0 28-29 40.25659579627917 41.0 40.5 41.0 39.0 41.0 30-31 40.138908135881394 41.0 40.0 41.0 38.5 41.0 32-33 40.04401394844352 41.0 40.0 41.0 38.0 41.0 34-35 39.994668064886646 41.0 40.0 41.0 38.0 41.0 36-37 39.92699652754644 41.0 40.0 41.0 38.0 41.0 38-39 39.83567273124595 41.0 40.0 41.0 38.0 41.0 40-41 39.76784795823462 41.0 40.0 41.0 38.0 41.0 42-43 39.613331103643276 41.0 40.0 41.0 37.5 41.0 44-45 39.50776638355821 41.0 40.0 41.0 37.0 41.0 46-47 39.40782208139346 41.0 40.0 41.0 37.0 41.0 48-49 39.25216262168911 41.0 40.0 41.0 37.0 41.0 50-51 39.189451922641304 41.0 40.0 41.0 36.5 41.0 52-53 39.21946546067973 41.0 40.0 41.0 36.0 41.0 54-55 39.17838497597531 41.0 39.5 41.0 36.0 41.0 56-57 39.131364943409395 41.0 39.0 41.0 36.0 41.0 58-59 39.12552200064492 41.0 39.0 41.0 35.0 41.0 60-61 38.993078411359164 41.0 39.0 41.0 35.0 41.0 62-63 38.83408840765486 41.0 39.0 41.0 35.0 41.0 64-65 38.62609830001679 41.0 38.0 41.0 35.0 41.0 66-67 38.38285332814553 40.0 37.0 41.0 35.0 41.0 68-69 38.07986509931004 39.5 37.0 41.0 35.0 41.0 70-71 37.76577261425385 39.0 36.0 41.0 35.0 41.0 72-73 37.4423287558063 39.0 36.0 41.0 35.0 41.0 74-75 37.06786141632399 37.5 35.0 40.5 35.0 41.0 76-77 35.8358166395284 36.5 34.5 39.0 32.5 40.0 78-79 36.196430141856254 37.0 35.0 39.0 34.0 41.0 80-81 35.915451219089704 36.0 35.0 39.0 34.0 39.5 82-83 35.54751438416582 36.0 35.0 37.0 34.0 39.0 84-85 35.18741855190959 35.5 35.0 37.0 34.0 39.0 86-87 34.90830710560469 35.0 35.0 37.0 34.0 38.0 88-89 34.65888873491295 35.0 35.0 36.0 34.0 37.0 90-91 34.465744498839406 35.0 35.0 36.0 34.0 37.0 92-93 34.325225389684974 35.0 35.0 36.0 34.0 36.5 94-95 34.19796782850397 35.0 35.0 35.0 34.0 36.0 96-97 34.103256857629404 35.0 35.0 35.0 33.0 36.0 98-99 34.03525619671729 35.0 35.0 35.0 33.0 36.0 100-101 33.79719525422464 35.0 34.5 35.0 32.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13239519185991355 1101 2 0.1256570265292538 1101 3 0.16365795667908145 1101 4 0.10555369113617274 1101 5 0.11152670892269612 1101 6 0.14551463800928843 1101 7 0.09712163437936994 1101 8 0.08289860065968213 1101 9 0.1441879324733648 1101 10-11 0.16321038337016347 1101 12-13 0.17258058325028713 1101 14-15 0.17495149777638375 1101 16-17 0.13989582948362056 1101 18-19 0.10214058663896708 1101 20-21 0.13566861310639666 1101 22-23 0.17776233009931985 1101 24-25 0.08737273373142074 1101 26-27 0.1359419831862283 1101 28-29 0.14032230136182733 1101 30-31 0.2459370179390632 1101 32-33 0.20934823914245726 1101 34-35 0.20666125874311092 1101 36-37 0.31844191392837473 1101 38-39 0.2999081865668387 1101 40-41 0.19908878820219655 1101 42-43 0.328027237686193 1101 44-45 0.3915818183824129 1101 46-47 0.3394080099385448 1101 48-49 0.32842556381600474 1101 50-51 0.29896982452723364 1101 52-53 0.3914589588391806 1101 54-55 0.31538283027248326 1101 56-57 0.36414582671189777 1101 58-59 0.4716071004598703 1101 60-61 0.3332271807628615 1101 62-63 0.25587176619553986 1101 64-65 0.31638937049861227 1101 66-67 0.19191039885857464 1101 68-69 0.25684833019168707 1101 70-71 0.292639814166499 1101 72-73 0.21910716323666435 1101 74-75 0.20672538184431488 1101 76-77 0.10203694734737923 1101 78-79 0.2588351681112826 1101 80-81 0.3250860019165316 1101 82-83 0.2370473257846939 1101 84-85 0.26639424869087946 1101 86-87 0.27095937799564496 1101 88-89 0.20377886714180704 1101 90-91 0.19095407215136362 1101 92-93 0.17740398190250772 1101 94-95 0.2581428003476489 1101 96-97 0.2758414543369767 1101 98-99 0.09269416205928849 1101 100-101 0.17710802371172463 1102 1 0.21320468723973818 1102 2 0.11187634307221828 1102 3 0.16348843142350233 1102 4 0.08359402831944607 1102 5 0.13732894419918296 1102 6 0.13115904281594482 1102 7 0.08338026112652841 1102 8 0.16412331007128245 1102 9 0.14148044215964006 1102 10-11 0.178747741577034 1102 12-13 0.12118249126932312 1102 14-15 0.14924508112261492 1102 16-17 0.16129163655896406 1102 18-19 0.06991850364038754 1102 20-21 0.06108732820323581 1102 22-23 -0.0042758548502703775 1102 24-25 0.030693561506048184 1102 26-27 0.046640255257067054 1102 28-29 0.1662989756694131 1102 30-31 0.07769671466083139 1102 32-33 0.1707357910749252 1102 34-35 0.13233671861107155 1102 36-37 0.03948810587518636 1102 38-39 0.1415249169897379 1102 40-41 0.09866718626224014 1102 42-43 0.1876369856340787 1102 44-45 0.21292553943720094 1102 46-47 0.28756399282877965 1102 48-49 0.39796285814378507 1102 50-51 0.40565280492864275 1102 52-53 0.48928854419023793 1102 54-55 0.3979354875194403 1102 56-57 0.4848502655710121 1102 58-59 0.5365279026828631 1102 60-61 0.46434882373284836 1102 62-63 0.47127940072856944 1102 64-65 0.5194179760428241 1102 66-67 0.499247402782224 1102 68-69 0.4987620983450114 1102 70-71 0.5054652596616762 1102 72-73 0.5307203803100649 1102 74-75 0.5415758433706799 1102 76-77 0.35347361246620324 1102 78-79 0.5162469842672763 1102 80-81 0.43809423986120066 1102 82-83 0.33395066444946764 1102 84-85 0.38465162733904634 1102 86-87 0.4550085255784424 1102 88-89 0.34361754789134835 1102 90-91 0.4306308439973989 1102 92-93 0.5811689167089895 1102 94-95 0.41377435563130405 1102 96-97 0.4461172891260148 1102 98-99 0.48771626176471017 1102 100-101 0.3565996444960078 1103 1 0.011522667859424018 1103 2 -0.008133227450571212 1103 3 0.040449790437740774 1103 4 0.005855064328535775 1103 5 0.03830274489304486 1103 6 0.04552679015414185 1103 7 0.04585902652908658 1103 8 0.04718587736402213 1103 9 0.0070193822242430315 1103 10-11 0.06841636222543457 1103 12-13 0.02922173041275755 1103 14-15 0.04818275309294506 1103 16-17 -0.050507621725522256 1103 18-19 0.012568342137811328 1103 20-21 0.0893227630638691 1103 22-23 0.001168576301779467 1103 24-25 0.0475173442551835 1103 26-27 0.08537933887950544 1103 28-29 0.0236993824166305 1103 30-31 0.1565470556107087 1103 32-33 0.12810628993842244 1103 34-35 0.09297794363799028 1103 36-37 0.07094282533923746 1103 38-39 0.0235562604518762 1103 40-41 0.05505670653978001 1103 42-43 0.18478018458132084 1103 44-45 0.2893676975900874 1103 46-47 0.018145402088258322 1103 48-49 0.07736589678476946 1103 50-51 0.22714700279919953 1103 52-53 0.29343975845031167 1103 54-55 0.3196725374057863 1103 56-57 0.22467201458405128 1103 58-59 0.1713992987828803 1103 60-61 0.28127190065592345 1103 62-63 0.2904288205156291 1103 64-65 0.15497486083765466 1103 66-67 0.17060282807233307 1103 68-69 0.11574977629692285 1103 70-71 0.21808519027538154 1103 72-73 0.2319908672104205 1103 74-75 0.12991775029734498 1103 76-77 -0.08981625474279298 1103 78-79 0.2734918700191642 1103 80-81 0.250746470989327 1103 82-83 0.20254738654541882 1103 84-85 0.23803600346059284 1103 86-87 0.19839649064602582 1103 88-89 0.10309834703000575 1103 90-91 0.17293973262043494 1103 92-93 0.12564313611321865 1103 94-95 0.2206364204715996 1103 96-97 0.173039169733741 1103 98-99 0.08492286542544036 1103 100-101 0.03918847091065203 1104 1 0.06211193593799891 1104 2 0.048831548144733006 1104 3 0.021944358637014716 1104 4 0.02250600254866697 1104 5 0.06961186373425932 1104 6 0.06995343341102966 1104 7 0.04536303179628476 1104 8 0.09024085377135549 1104 9 0.0876426676406794 1104 10-11 0.07980887841110729 1104 12-13 0.1693951091025454 1104 14-15 0.1348677086509369 1104 16-17 0.034975753672263465 1104 18-19 0.03026064983011878 1104 20-21 0.08645433334807251 1104 22-23 0.059154969366630894 1104 24-25 -0.04618505890513802 1104 26-27 0.019732460793264295 1104 28-29 0.013047566329724702 1104 30-31 0.07344928319766808 1104 32-33 0.10702431912749688 1104 34-35 0.11240074622769924 1104 36-37 0.08306726295582934 1104 38-39 0.08162714819466288 1104 40-41 0.10781430557413074 1104 42-43 0.18188980819594036 1104 44-45 0.12588037179178002 1104 46-47 0.15355525614290855 1104 48-49 0.07841823959174832 1104 50-51 0.16025465121354898 1104 52-53 0.15344195311727304 1104 54-55 0.17722767841313924 1104 56-57 0.22986349927624872 1104 58-59 0.1833898617149501 1104 60-61 0.18688805340474346 1104 62-63 0.17648280932282745 1104 64-65 0.14281201654927145 1104 66-67 0.17636163417350303 1104 68-69 0.12795212459056415 1104 70-71 0.13238015351470978 1104 72-73 0.06646469580692838 1104 74-75 0.09089108509379429 1104 76-77 -0.08529304613969657 1104 78-79 0.17339640200643913 1104 80-81 0.1877353879211796 1104 82-83 0.19039990492575498 1104 84-85 0.23254055739452895 1104 86-87 0.2051055875405794 1104 88-89 0.20410789383127792 1104 90-91 0.2182903306671804 1104 92-93 0.17087302747720656 1104 94-95 0.055551377126924706 1104 96-97 0.10139646151471027 1104 98-99 0.2001044741163156 1104 100-101 0.24545205041246732 1105 1 -0.05343530639315475 1105 2 -0.004009384488554701 1105 3 -0.03410282154225541 1105 4 -0.020422199210138103 1105 5 -0.153594265427202 1105 6 -0.07727350774989361 1105 7 -0.05989264918109427 1105 8 -0.08716032967927845 1105 9 -0.06697146786618902 1105 10-11 -0.04437648807779482 1105 12-13 0.11526279861151068 1105 14-15 0.12202890693910007 1105 16-17 0.11267250381975202 1105 18-19 0.05792316711919199 1105 20-21 0.08850139908897603 1105 22-23 0.011849499804277741 1105 24-25 0.05002505784172939 1105 26-27 0.05947019960937894 1105 28-29 0.0321992228251915 1105 30-31 0.05560972185180191 1105 32-33 0.01508788372499481 1105 34-35 0.05712306545946433 1105 36-37 0.044160188888547225 1105 38-39 0.14767584764200592 1105 40-41 0.029462494389555616 1105 42-43 0.08952324235342246 1105 44-45 0.15246164375457028 1105 46-47 0.15542277202123245 1105 48-49 0.16302075368444235 1105 50-51 0.16532446848704296 1105 52-53 0.07560807422859028 1105 54-55 0.07573169263506685 1105 56-57 0.018387303444569625 1105 58-59 0.07561357689756676 1105 60-61 0.061167634244604585 1105 62-63 0.045725128633513634 1105 64-65 -0.04332495829203964 1105 66-67 -0.07979189477052273 1105 68-69 -0.05510882568201225 1105 70-71 -0.059890126247651665 1105 72-73 -0.06055302043720445 1105 74-75 -0.06674678848337834 1105 76-77 -0.10396031262128957 1105 78-79 0.05640388193533141 1105 80-81 0.06848665611539673 1105 82-83 0.11986837814026785 1105 84-85 0.19288864735887756 1105 86-87 0.17703464364102928 1105 88-89 0.05889151651611968 1105 90-91 0.045047403649270734 1105 92-93 0.13672303823047116 1105 94-95 0.1414620402183786 1105 96-97 0.19131345423209467 1105 98-99 0.17221406661705885 1105 100-101 0.08059074774862296 1106 1 0.08383855436971999 1106 2 0.1174552187237623 1106 3 0.06926880098777843 1106 4 0.06851408307277751 1106 5 0.15622961163482785 1106 6 -0.03707077207058518 1106 7 0.07653402396839937 1106 8 0.08727191649133914 1106 9 0.09712693113338844 1106 10-11 0.08330113125135341 1106 12-13 0.14331575888762416 1106 14-15 0.01350693623337662 1106 16-17 0.09826939353618513 1106 18-19 0.09350965116167487 1106 20-21 0.06539159436571396 1106 22-23 0.058059900689777066 1106 24-25 0.07625748560517565 1106 26-27 0.0925758973144184 1106 28-29 0.07680088810135999 1106 30-31 0.046461016483391404 1106 32-33 -0.005522717538788413 1106 34-35 0.06148147738786491 1106 36-37 0.08896904678756101 1106 38-39 0.0113366558221486 1106 40-41 -0.005090789107043747 1106 42-43 0.11657983632770907 1106 44-45 0.06117349498843083 1106 46-47 -0.26307041272276166 1106 48-49 -0.07529312195946858 1106 50-51 -0.06022265816567085 1106 52-53 -0.21550216513875142 1106 54-55 0.025183138655407333 1106 56-57 0.02184603896180448 1106 58-59 -0.0334793245910916 1106 60-61 0.0069586772614798065 1106 62-63 -0.06252518952226893 1106 64-65 -0.03972349007906928 1106 66-67 -0.022884829409996144 1106 68-69 -0.2572708116371132 1106 70-71 -0.049048887364683935 1106 72-73 -0.12066098160654093 1106 74-75 -0.01585199260954795 1106 76-77 -0.143568175259027 1106 78-79 -0.05649583867693053 1106 80-81 -0.03438309983931731 1106 82-83 -0.005503417510716702 1106 84-85 -0.0072861311291845254 1106 86-87 -0.011924107530759898 1106 88-89 -0.06211726292594477 1106 90-91 -0.06307583636451142 1106 92-93 0.047611793288275805 1106 94-95 0.1733743084158803 1106 96-97 0.08496378306981 1106 98-99 0.05313448070762661 1106 100-101 0.02527676344369212 1107 1 0.06497424219183046 1107 2 0.04214975381686514 1107 3 0.10431188689051396 1107 4 0.025412656994937777 1107 5 0.032527535646337924 1107 6 0.11778665464198212 1107 7 0.09204874354782078 1107 8 0.06989612048877092 1107 9 0.0851222120369215 1107 10-11 0.06757339690060604 1107 12-13 0.09695788027326557 1107 14-15 0.12591491730179172 1107 16-17 0.04168249385838152 1107 18-19 -0.009247122190608081 1107 20-21 -0.030503861089187012 1107 22-23 0.022836571518993765 1107 24-25 0.07541491300766978 1107 26-27 0.04345227630477666 1107 28-29 -0.007641789364953411 1107 30-31 0.041816087097352295 1107 32-33 0.01788907192327116 1107 34-35 -0.012043977446104748 1107 36-37 -0.03564545684489673 1107 38-39 -0.005250036877740172 1107 40-41 -0.05024819581174711 1107 42-43 0.19873195819630496 1107 44-45 0.12367220137645774 1107 46-47 0.13809159579093233 1107 48-49 0.06819690515464316 1107 50-51 -0.027583965714157443 1107 52-53 -0.09581891034203949 1107 54-55 -0.05096317403302919 1107 56-57 -0.15448103443946337 1107 58-59 -0.07496977256028714 1107 60-61 -0.09588433688891485 1107 62-63 0.01979510190263767 1107 64-65 0.017372230307564962 1107 66-67 -0.022097450923290296 1107 68-69 0.07692952918651486 1107 70-71 0.044543938780769565 1107 72-73 0.06542251328455251 1107 74-75 -0.005524657036254155 1107 76-77 -0.2147823767037309 1107 78-79 -0.11717572663288678 1107 80-81 -0.10680036041760843 1107 82-83 -0.008233801178768374 1107 84-85 -0.09623661511254511 1107 86-87 -0.13120892984021992 1107 88-89 -0.04267686221670175 1107 90-91 -0.18111965358921367 1107 92-93 -0.14289412972973992 1107 94-95 -0.10003316900628079 1107 96-97 -0.06648454489359779 1107 98-99 -0.09300658854028399 1107 100-101 -0.061503039651320535 1108 1 0.024384831076922353 1108 2 -0.009933305545061444 1108 3 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2315 92-93 -0.19043273733107213 2315 94-95 -0.19235053409430236 2315 96-97 -0.1590480997219501 2315 98-99 -0.1324335598257278 2315 100-101 -0.5732961594687112 2316 1 -0.10280425521749947 2316 2 -0.14079806261540284 2316 3 -0.149028231540278 2316 4 -0.09663119940772447 2316 5 0.010789008629309649 2316 6 0.0010212956486483904 2316 7 -0.04180580863575045 2316 8 -0.32917401274586666 2316 9 -0.18704655184168928 2316 10-11 -0.11496962678555178 2316 12-13 -0.0582095882685536 2316 14-15 -0.08109609306089993 2316 16-17 -0.17453234700024467 2316 18-19 -0.3134893501698812 2316 20-21 -0.27196157759547646 2316 22-23 -0.2464893907311918 2316 24-25 -0.27361589750305626 2316 26-27 -0.24589126551609297 2316 28-29 -0.2572346835174315 2316 30-31 -0.589771625707975 2316 32-33 -0.7975393144571825 2316 34-35 -0.7094602431751937 2316 36-37 -0.6191808010343891 2316 38-39 -0.47743961866900264 2316 40-41 -0.42364521653713894 2316 42-43 -0.3200481121219738 2316 44-45 -0.3080704892230912 2316 46-47 -0.3526444330765699 2316 48-49 -0.38648712519324846 2316 50-51 -0.2703323928052015 2316 52-53 -0.4002415602310023 2316 54-55 -0.40951702303377147 2316 56-57 -0.44063018321813985 2316 58-59 -0.4392531737445964 2316 60-61 -0.6811868905528655 2316 62-63 -0.7577030476162392 2316 64-65 -0.5561034358656443 2316 66-67 -0.547213105993599 2316 68-69 -0.6153285204063721 2316 70-71 -0.5659876119224165 2316 72-73 -0.39148440751485936 2316 74-75 -0.36283637058177476 2316 76-77 -0.3662997712263021 2316 78-79 -0.44851362448633125 2316 80-81 -0.28125215538430126 2316 82-83 -0.25662156949854165 2316 84-85 -0.3131384470888605 2316 86-87 -0.34200186100232344 2316 88-89 -0.3813796749480147 2316 90-91 -0.40765092672022973 2316 92-93 -0.21854642432634108 2316 94-95 -0.344123194913017 2316 96-97 -0.3392616544420832 2316 98-99 -0.5832776840251128 2316 100-101 -0.7065807598585749 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 3.0 14 18.0 15 45.0 16 105.0 17 255.0 18 550.0 19 854.0 20 1299.0 21 1984.0 22 2685.0 23 2583.0 24 2185.0 25 1759.0 26 1720.0 27 1768.0 28 2152.0 29 2836.0 30 2707.0 31 3361.0 32 4441.0 33 5915.0 34 8742.0 35 15602.0 36 34964.0 37 109407.0 38 316933.0 39 225337.0 40 267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.05604108790662 22.940570040023633 14.959049462061571 22.044339410008178 2 13.324681000756858 23.067882612542505 42.9271178671556 20.680318519545033 3 15.600590556951701 29.904407590895403 33.47586471555462 21.019137136598275 4 10.798850865714918 19.68731928184437 43.43165289322272 26.08217695921799 5 10.795296312335521 40.77684133382191 37.286118791432095 11.141743562410474 6 25.291214399356143 42.23494892588457 20.771694839822086 11.702141834937201 7 23.01106761290804 35.381583470801274 26.448876582657988 15.158472333632696 8 22.98241920482679 37.537809236246765 23.419340740168266 16.060430818758178 9 25.379291598154772 16.78356993809279 23.440660485717103 34.396477978035335 10-11 21.510357398884445 28.33314234394613 32.515410178579515 17.64109007858991 12-13 22.684609009191476 26.558206902800613 31.46794443008323 19.289239657924682 14-15 20.477415727043297 28.070976097900274 28.930694837157116 22.520913337899312 16-17 19.588981955500362 31.31004506461215 29.476880068436383 19.6240929114511 18-19 19.453165184436987 30.229756704679616 31.298321264954442 19.018756845928955 20-21 20.831767606103003 29.451728962622425 30.783063495236192 18.933439936038376 22-23 20.464757128123676 29.456559685960148 30.25178619493176 19.826896990984412 24-25 20.039361580219538 29.812932557649923 30.42560874535962 19.72209711677091 26-27 20.103402901544047 29.677165747788862 30.531572197145096 19.687859153521995 28-29 19.893254699005166 29.477679558894465 30.8183584328921 19.810707309208265 30-31 20.672558023019995 29.428111150493418 30.237128869866936 19.662201956619647 32-33 20.375480693637922 29.44756541830673 30.21354390135354 19.96340998670181 34-35 20.308856488797804 29.634379688678415 30.41927944589981 19.637484376623966 36-37 20.66487486957947 29.22612488123903 30.107321272238117 20.00167897694338 38-39 20.198277068217703 29.696654740727418 30.343320474639057 19.761747716415822 40-41 20.07893635789457 29.307254842913448 30.675649386124576 19.938159413067407 42-43 20.542574199376933 29.177271019270385 30.094286574689733 20.185868206662956 44-45 20.568091269830692 29.408190513246225 29.854506061470154 20.169212155452925 46-47 20.506976930150735 29.303439819812617 29.779696933349324 20.409886316687324 48-49 20.21165529927758 29.391491014576953 30.014504330978575 20.38234935516689 50-51 20.18879967167592 29.368748983980876 29.685147332766583 20.757304011576622 52-53 20.37448133056532 29.426911914806297 29.80926822638372 20.389338528244664 54-55 20.526384517600782 29.415652424188316 29.645639179296396 20.412323878914503 56-57 20.81386796148588 29.511991024387125 29.235034204866768 20.43910680926023 58-59 20.37907840069929 29.456293189140787 29.687812300960186 20.476816109199735 60-61 20.893927330510593 29.480546736802125 29.579220536942714 20.046305395744568 62-63 20.330701641581307 29.64800984002628 29.659803479323482 20.36148503906893 64-65 20.39371810077158 29.396796417958182 29.623872283151876 20.585613198118363 66-67 20.592607201179565 29.64515125328721 29.220870508423985 20.54137103710924 68-69 20.662834787000214 29.629553133184576 29.2874955777035 20.42011650211171 70-71 20.520405477882267 29.068087105123773 29.908224485240964 20.503282931752995 72-73 20.63935495519745 29.33089660401394 29.36587517381018 20.66387326697843 74-75 20.401725067241777 29.41715353245028 29.192958751491577 20.988162648816363 76-77 20.26774298645428 29.502364221339356 29.605367918412544 20.62452487379382 78-79 20.504665026822906 29.633846695039693 29.20285471392899 20.65863356420841 80-81 20.596206684273223 29.49073790304313 29.072004775623 20.841050637060647 82-83 20.54290732040113 29.705667587857338 29.184000063959235 20.567425027782292 84-85 20.827192802453904 29.043223119132072 29.297461084801952 20.83212299361207 86-87 20.05572459630648 29.49592692109613 29.721583553915416 20.726764928681977 88-89 20.711397984718825 29.02529894758723 29.263684164239283 20.999618903454653 90-91 20.519645263956686 29.36812559004415 29.524959908644345 20.587269237354818 92-93 20.73411878829226 29.5425049102039 29.294929365018028 20.428446936485813 94-95 20.57053725569368 29.11693588418683 29.386319978731784 20.92620688138771 96-97 20.71479776888863 29.085129743976506 29.456493061755307 20.743579425379558 98-99 20.54730451792058 29.19059586023841 29.433174590061267 20.828925031779747 100-101 20.377879165012168 29.506927584819277 29.337768728730225 20.777424521438338 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 48.0 1 32.5 2 27.5 3 39.5 4 31.0 5 29.0 6 47.5 7 80.5 8 163.0 9 222.5 10 192.5 11 155.5 12 151.0 13 174.0 14 215.0 15 247.0 16 255.5 17 284.0 18 337.5 19 372.0 20 427.0 21 500.5 22 598.5 23 753.0 24 952.0 25 1369.5 26 2515.5 27 4448.5 28 7399.5 29 11698.5 30 16655.0 31 21881.5 32 28209.0 33 33855.0 34 38430.0 35 42447.5 36 46464.5 37 47656.5 38 42658.5 39 36908.5 40 31982.5 41 27589.5 42 24036.5 43 21449.0 44 20111.0 45 19323.0 46 18692.5 47 17487.5 48 16774.0 49 16486.0 50 15801.0 51 15064.0 52 14306.5 53 13512.5 54 12798.0 55 11783.0 56 10819.0 57 9769.0 58 8774.5 59 7859.0 60 6576.0 61 5251.0 62 4124.0 63 3118.5 64 2251.5 65 1623.0 66 1155.0 67 866.5 68 649.0 69 460.0 70 331.5 71 242.0 72 177.5 73 117.5 74 69.0 75 49.0 76 36.5 77 22.0 78 9.5 79 7.5 80 8.0 81 2.0 82 1.5 83 1.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08940968289543463 2 7.994904580813829E-4 3 0.0 4 0.0 5 3.9974522904069143E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.004263949109767374 20-21 0.003730955471046453 22-23 0.0 24-25 0.0 26-27 0.0020653503500435723 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0027982166032848397 38-39 0.001732229325842996 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.01972076463267411 48-49 0.001665605121002881 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.003397834446845877 62-63 0.00979375811149694 64-65 0.009060891858255673 66-67 0.0041973249049272595 68-69 0.0025983439887644942 70-71 7.328662532412676E-4 72-73 0.0024650955790842637 74-75 0.0019321019403633418 76-77 0.0025983439887644942 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.9987261452034572E-4 88-89 0.0015989809161627657 90-91 5.996178435610371E-4 92-93 0.0 94-95 0.008328025605014404 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 750478.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.41891995117772 #Duplication Level Percentage of deduplicated Percentage of total 1 69.51533384273195 33.65857384710634 2 15.581102469864867 15.088403064789688 3 6.099879657955159 8.860487546110447 4 2.8679871193090496 5.554593550033346 5 1.6669429581830173 4.035578882772146 6 1.0193215073743112 2.961266788204235 7 0.6538038915522979 2.2159534802187433 8 0.4840803473696984 1.8750918071385383 9 0.3471873305320132 1.5129392008583364 >10 1.5833277529646768 13.347457573380945 >50 0.10922995547217611 3.6818851447619227 >100 0.06903084365255491 6.145047346159027 >500 0.0022178584305307027 0.7373412497391659 >1k 5.544646076326757E-4 0.32538051872705953 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1225 0.16322930185828233 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1199 0.1597648432065963 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.1268524860155794 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 824 0.1097966895765099 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.3324840968023048E-4 1.3324840968023048E-4 5 0.0 0.0 0.0 1.3324840968023048E-4 1.3324840968023048E-4 6 0.0 0.0 0.0 1.3324840968023048E-4 2.6649681936046095E-4 7 0.0 0.0 0.0 1.3324840968023048E-4 2.6649681936046095E-4 8 0.0 0.0 0.0 2.6649681936046095E-4 2.6649681936046095E-4 9 0.0 0.0 0.0 9.327388677616132E-4 3.997452290406914E-4 10-11 0.0 0.0 0.0 9.327388677616132E-4 3.997452290406914E-4 12-13 0.0 0.0 0.0 9.993630726017285E-4 3.997452290406914E-4 14-15 0.0 0.0 0.0 0.0013324840968023048 3.997452290406914E-4 16-17 0.0 0.0 0.0 0.0013991083016424198 3.997452290406914E-4 18-19 0.0 0.0 0.0 0.0017322293258429959 3.997452290406914E-4 20-21 0.0 0.0 0.0 0.001998726145203457 3.997452290406914E-4 22-23 0.0 0.0 0.0 0.002998089217805185 3.997452290406914E-4 24-25 0.0 0.0 0.0 0.003730955471046453 3.997452290406914E-4 26-27 0.0 0.0 0.0 0.004796942748488297 3.997452290406914E-4 28-29 6.662420484011524E-5 0.0 0.0 0.006196051050130717 3.997452290406914E-4 30-31 1.3324840968023048E-4 0.0 0.0 0.009993630726017284 3.997452290406914E-4 32-33 1.3324840968023048E-4 0.0 0.0 0.018255032126191575 3.997452290406914E-4 34-35 1.3324840968023048E-4 0.0 0.0 0.030114140587732083 3.997452290406914E-4 36-37 1.3324840968023048E-4 0.0 0.0 0.048502421123603895 3.997452290406914E-4 38-39 1.3324840968023048E-4 0.0 0.0 0.07641796295161217 3.997452290406914E-4 40-41 1.3324840968023048E-4 0.0 0.0 0.10026942828437342 3.997452290406914E-4 42-43 1.3324840968023048E-4 0.0 0.0 0.12611961976233813 3.997452290406914E-4 44-45 1.3324840968023048E-4 0.0 0.0 0.15510114886778825 3.997452290406914E-4 46-47 1.3324840968023048E-4 0.0 0.0 0.19254395198793303 3.997452290406914E-4 48-49 1.3324840968023048E-4 0.0 0.0 0.23285159591620275 5.329936387209219E-4 50-51 1.3324840968023048E-4 0.0 0.0 0.2658305773120598 5.329936387209219E-4 52-53 1.3324840968023048E-4 0.0 0.0 0.2992759281417976 5.329936387209219E-4 54-55 1.3324840968023048E-4 0.0 0.0 0.334187011478018 5.329936387209219E-4 56-57 1.3324840968023048E-4 0.0 0.0 0.3784921076966946 5.329936387209219E-4 58-59 1.3324840968023048E-4 0.0 0.0 0.42666140779609796 5.329936387209219E-4 60-61 1.3324840968023048E-4 0.0 0.0 0.4716993702680159 5.329936387209219E-4 62-63 1.3324840968023048E-4 0.0 0.0 0.5125400078350064 5.329936387209219E-4 64-65 1.3324840968023048E-4 0.0 0.0 0.5553793715472006 5.329936387209219E-4 66-67 1.3324840968023048E-4 0.0 0.0 0.6044814105143655 5.329936387209219E-4 68-69 1.3324840968023048E-4 0.0 0.0 0.6557154240364141 5.329936387209219E-4 70-71 1.3324840968023048E-4 0.0 0.0 0.7138783548618347 5.329936387209219E-4 72-73 1.3324840968023048E-4 0.0 0.0 0.7681104576016886 5.329936387209219E-4 74-75 1.3324840968023048E-4 0.0 0.0 0.8244079106915859 6.662420484011524E-4 76-77 1.3324840968023048E-4 0.0 0.0 0.8829039625412072 7.328662532412676E-4 78-79 1.3324840968023048E-4 0.0 0.0 0.947596065440959 7.994904580813828E-4 80-81 1.3324840968023048E-4 0.0 0.0 1.0141536460762341 8.661146629214979E-4 82-83 1.3324840968023048E-4 0.0 0.0 1.0873070229906805 9.327388677616132E-4 84-85 1.3324840968023048E-4 0.0 0.0 1.158528297964764 9.327388677616132E-4 86-87 1.3324840968023048E-4 0.0 0.0 1.2352793819405765 0.0010659872774418438 88-89 1.3324840968023048E-4 0.0 0.0 1.319492376858482 0.0011992356871220744 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 765 0.0 62.1483 1 GTATCAA 1785 0.0 47.410275 1 TCAACGC 2040 0.0 40.976883 4 CAACGCA 2105 0.0 39.9372 5 AACGCAG 2125 0.0 39.33781 6 ATCAACG 2125 0.0 39.1143 3 TATCAAC 2180 0.0 38.345345 2 ACGCAGA 2385 0.0 34.45198 7 CGCAGAG 2445 0.0 33.60653 8 GCAGAGT 2910 0.0 27.909983 9 GTACATG 2985 0.0 27.076674 1 ACATGGG 2710 0.0 26.815048 3 TCTGACG 45 8.953301E-4 26.386631 86-87 CATGGGG 1110 0.0 26.10137 4 TACATGG 3400 0.0 22.630417 2 AGAGTAC 2540 0.0 22.626017 10-11 GTCTTAC 170 3.1216932E-6 22.37339 1 CAGAGTA 2740 0.0 22.187881 10-11 CATGGGA 1525 0.0 22.112864 4 GAGTACT 1335 0.0 21.525091 12-13 >>END_MODULE