FastQCFastQC Report
Fri 10 Feb 2017
SRR2031420_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031420_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618121
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.11562794129734426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.050.6979141
GTATCAA29650.044.49771
TCAACGC35550.036.4564934
ATCAACG36000.036.000793
CAACGCA36950.035.2036745
AACGCAG37550.035.0063636
TATCAAC39900.032.476892
ACGCAGA44500.029.112527
GACCGTA1004.9868264E-528.4726857
CGCAGAG45950.028.0905698
GTACATG50050.027.2141821
TACATGG50250.026.54332
ACATGGG51650.024.8167743
GCAGAGT56550.022.5733939
CATGGGG40550.022.2440874
AGAGTAC46050.020.14438410-11
ACCGTAT2601.5077603E-820.0768938
CAGAGTA51100.019.63931510-11
GAGTACT25000.018.60042812-13
GTATTAA10750.018.5420741
Produced by FastQC (version 0.11.5)