FastQCFastQC Report
Fri 10 Feb 2017
SRR2031420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618121
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26050.1609891967287984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.12465075232321934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.053.2299461
GTATCAA33400.045.9706341
TCAACGC39700.038.4040034
ATCAACG40300.037.4786573
CAACGCA40550.037.2475935
AACGCAG41450.036.553436
TATCAAC41700.036.220382
ACGCAGA48700.031.0141667
CGCAGAG50600.029.7557358
GTACATG50550.028.96371
ACATGGG50350.027.6394943
TACATGG52500.026.688532
GCAGAGT61350.024.2321389
CATGGGG39250.023.3549884
ACCGGTC1553.9098857E-521.450058
AGAGTAC51550.021.19147310-11
CAGAGTA55400.021.17625410-11
GAGTACT31400.020.95055412-13
GTATCCG1456.15054E-419.653749
GTACTTT33550.019.61039414-15
Produced by FastQC (version 0.11.5)